Protein recognition by bivalent, ‘turn-on’ fluorescent molecular probes

Abstract

The selective and sensitive identification of different proteins becomes possible by modifying the known intercalating dye, thiazole orange, with two protein binders. These ‘turn-on’ fluorescence probes enable the identification of acetylcholinesterase, glutathione-s-transferases and avidin with high affinity, specificity, and high signal-to-noise ratio.

A Miniaturized Therapeutic Chromophore for Multiple Metal Pollutant Sensing, Pathological Metal Diagnosis and Logical Computing

“…Hence, therapeutic chromophore 1 competes efficiently with multiple-dye integrated sensors or devices, multichannel sensors and array based sensors, and takes analytical device miniaturization6789101112131415161718192021222324252627282930313233343536373848495051525354555657585960 a step further with easy accessibility and diversification using an already existing concept of pattern generation and chemometric analysis. The multi-metal receptive nature of the unimolecular therapeutic device 1 further increases its potential for the construction of a next generation complex logic gate device at the molecular level123456789101112. Apart from its remarkably small size, this unimolecular sensor operates fast and in single mode, i.e.…”

“…The designing of analytical systems to sense multianalytes at the molecular level12345 and exploiting them as computational devices to mimic logic gates and circuits678910111213 has attracted special attention in the last decades. Ever since the invention of the “lab on a molecule” technology using two metal ions and proton, based on the “receptor-per-analyte” paradigm by A. P. de Silva6, many diagnostic devices were developed to detect multiple metals with high sensitivity14151617.…”

“…Likewise, Eric T. Kool and coworkers developed a very elegant concept of tetramer-length oligodeoxy-fluoroside (ODF) sensor sequences attached to microbeads, each of which contains four different dye units for the detection of various metal ions484950. These examples1234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950 demonstrate that modern approaches aimed at high analytical capacity and easy applicability have markedly advanced multi-analyte detection and molecular computation. Still, there is scope for an alternative sensor to both concepts of (1) a multi-dye integrated sensor that involve tedious synthetic assembling or (2) a single-molecule driven multichannel sensor, based on diverse measuring parameters.…”

ECBS & ICBS 2015 Joint Meeting: Bringing Chemistry to Life

“…David Margulies (Weizmann Institute, Rehovot) presented synthetic receptors that communicate with proteins thereby allowing protein detection in mixtures . He described the use of optical cross‐reactive sensor arrays, the so‐called chemical “noses/tongues” approach, for the detection and analysis of proteins.…”

Lighting-up protein–ligand interactions with fluorescent PET (photoinduced electron transfer) sensor designs

“…13 We and others 8,9 had demonstrated that apolar environments can cause strong switching 'on' of fluorescence by retarding the competing charge-separating PET process. Fine examples of selective 'off-on' fluorescent biotin-based sensors have been designed for avidin on the basis of polarity-sensitive ICT (internal charge transfer) excited states (one of which involves a separate PET process involving a thiol reaction), 14 torsionally-restricted thiazole orange derivatives 15 and disaggregation. 16 There is a pseudointramolecular PET pair which dissociates upon engulfment of one partner by avidin so that the PET process ceases.…”