Lighting-up protein–ligand interactions with fluorescent PET (photoinduced electron transfer) sensor designs

Abstract

The fluorescence of sensors 1 and 6, which are designed in terms of photoinduced electron transfer (PET), is switched 'on' by factors of 15 and 6 with avidin in a rather selective manner. Sensors 3 and 7, simpler derivatives of 1, are switched 'on' by factors of 16 and 7.5 with bovine serum albumin, though at 30-fold higher concentrations.

A highly sensitive fluorescent 1,8‐naphthalimide Schiff base probe for detection of Hg2+

“…The photoinduced electron transfer (PET) process that is favored in polar solvents could explain this fact and the fluorescence emission is lower. [ 57 ] The obtained Stokes shift values are in the 3,445–4,049 cm −1 regions. It is seen that the Stokes shift depends on the media and it is larger in the case of polar solvents when the hydrogen bond formation or dipole–dipole interactions are favored in comparison to non‐polar media.…”

Glycoform Differentiation by a Targeted, Self-Assembled, Pattern-Generating Protein Surface Sensor

“…These processes can, in turn, enhance An's monomer fluorescence by disrupting PET, [12][13][14]45,46 internal conversion, 48 or excimer formation, 47 respectively (Figure 2b). Our expectation that such processes can occur on protein surfaces is based on previous studies by us and others, showing that intramolecular motion, 51 PET, 52 ICT, 2,7 and excimer emission 53,54 can be affected by the binding of fluorescent probes to protein surfaces. The reason for incorporating three An-BA units in the structure of ODN-2 (Figure 1d) is to cover a larger surface area and, more importantly, to induce the formation of intramolecular An-An interaction, which would lead to excimer emission at low sensor concentrations.…”

Multiple molecular logic gate arrays in one system of (2-(2′-pyridyl)imidazole)Ru(ii) complexes and trimeric cyclophanes in water

“…Luminescent switching/sensing/logic research has been driven more by atomic rather than by molecular inputs, [1][2][3][4][5][6] in terms of numbers of publications, although notable exceptions concerning sugars, proteins and oligonucleotides do exist. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] This situation has arisen because of the dearth of suitable receptors/ hosts for molecular targets cf. those for atomic counterparts.…”