Protein patterning

Blawas, Amy S.; Reichert, William M.

doi:10.1016/s0142-9612(97)00218-4

Cited by 508 publications

(379 citation statements)

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“…It is well established that the organization of the adsorbed protein layer (composition, quantities and conformation) depends on the physicochemical properties of the polymer surface [33,34]. The protein adsorption experiments showed that both PET (reference membrane) and PEI adsorbed the highest amount of FN (Fig.…”

Section: Protein Adsorptionmentioning

confidence: 92%

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Tzoneva

Seifert

Albrecht

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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Surface modifications of poly(ether imide) (PEI) membranes with carboxylic groups were tested in comparison to pure PEI and poly(ethylene terephtalate) (PET) for their ability to support attachment, growth and function of human umbilical vein endothelial cells (HUVEC) in respect to endothelization of the above materials. Flat sheet PEI membranes were modified by covalent binding of iminodiacetic acid (IDA) for different periods of time (1 to 30 min) to obtain surfaces with various content of carboxylic groups. In addition, fibronectin (FN) and fibrinogen (FNG) preadsorption on the various membranes were studied on their effect on HUVEC behaviour. Results show a decreased protein adsorption and HUVEC adhesion, growth and function in terms of prostacyclin production with an increase carboxylic groups.Preadsorption of the membranes with FN or FNG promoted activity of HUVEC, which became superior to cells on PET. FN-coated membranes were found to be better substrate for HUVEC adhesion and prostacyclin production, while on FNG-coated membranes cells grew better. Overall it can be concluded that PEI is a promising materials for endothelial cells immobilization as it is needed for improving the hemocompatibility of cardiovascular devices.

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Section: Protein Adsorptionmentioning

confidence: 92%

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Tzoneva

Seifert

Albrecht

et al. 2008

Journal of Biomaterials Science, Polymer Edition

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“…These compounds come in a variety of chemistries that are used for many applications and are often utilized to modulate the biological interactions of silica substrates with biological materials. 11 Work has shown that it is possible to pattern the aminated alkylsilane DETA using deep-ultraviolet ͑DUV͒ photolithography. [12][13][14][15][16] Using DUV, SAMs were exposed to intense ultraviolet light from an ArF excimer laser ͑emission wavelength 193 nm͒.…”

Section: Introductionmentioning

confidence: 99%

Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture

Wilson

Stancescu

Das

et al. 2011

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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This manuscript details a general method for patterning coplanar alkylsilane monolayers using deep-ultraviolet photolithography that has broad application for high fidelity patterning of cells of varying phenotype in long-term cultures. A polyethylene glycol monolayer was formed on a silica substrate and then patterned using 193 nm light from an ArF excimer laser. The regions of photoablation were then rederivatized with ͑3-trimethoxysilyl propyl͒ diethyltriamine ͑DETA͒, yielding high contrast cytophilic islands that promoted cell adhesion and growth. Rat hippocampal neurons, motoneurons, and myoblasts were then cultured in a defined, serum-free medium on the patterned surfaces for periods in excess of 40 days. This approach has been shown to be useful as a general method for the long-term culture of multiple cell types in highly defined spatial patterns and can be used for supporting complex cocultures for creating in vitro models for biological systems.

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“…For in situ regeneration, a scaffold implanted directly into the injured tissue stimulates the body's own cells to promote local tissue repair [657,759]. In any case, simply trapping cells at the particular point on a surface is not enough: the cells must be encouraged to differentiate, which is impossible without the presence of suitable biochemical factors [760]. All previously mentioned clearly indicates that for the purposes of tissue engineering, calcium orthophosphate bioceramics plays an auxiliary role; namely, it acts as a suitable material to manufacture the appropriate 3D templates, substrates or scaffolds to be colonized by living cells before the successive implantation [761,762].…”

Section: Bioceramic Scaffolds From Calcium Orthophosphatesmentioning

confidence: 99%

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2011

BIO

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In the late 1960's, a strong interest was raised in biomedical applications of ceramic materials (named bioceramics a little bit later). Bioceramics was initially used as reasonable alternatives to metals in order to increase their biocompatibility. However, within a short period, it has grown into a diverse class of biomaterials, presently including three essential types: relatively bioinert, bioactive (or surface reactive) and bioresorbable bioceramics. This review is limited to bioceramics prepared from calcium orthophosphates only, which belong to the categories of bioactive and bioresorbable compounds. There have been a number of important advances in this field during the past 30 -40 years. The research was shifted from an initial study on the develop-ment of bioinert bioceramics towards bioactive and bioresorbable bioceramics, and these different types of calcium orthophosphate bioceramics can be tuned through the structural and compositional control. At the turn of the millennium, a new concept of calcium orthophosphate bioceramics has been developed to promote a regeneration of bones. Current biomedical applications of calcium orthophosphate bioceramics include artificial replacements for hips, knees, teeth, tendons and ligaments, as well as repair for periodontal disease, maxillofacial reconstruction, augmenttation and stabilization of the jawbone, spinal fusion and bone fillers after tumor surgery. Potential future applications of calcium orthophosphate bioceramics are demonstrated in drug delivery systems, effective carriers of growth factors, bioactive peptides and/or various types of cells for tissue engineering purposes.

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Protein patterning

Cited by 508 publications

References 0 publications

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture

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