Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Tzoneva, Rumiana; Seifert, B.; Albrecht, Wolfgang; Richau, K.; Lendlein, Andreas; Groth, Thomas

doi:10.1163/156856208784613523

Cited by 20 publications

(15 citation statements)

References 43 publications

(43 reference statements)

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“…Tzoneva et al reported that human umbilical venous endothelial cells (HUVEC) were able to adhere and proliferate on PEI-membranes. 37 In a comparative study between PEI-membranes and -films, Braune et al showed that platelet adherence can strongly be reduced by the processing of PEI (by changing surface roughness). 6 Therefore, this study was performed to investigate the behavior of HUVEC seeded on smooth PEIfilms (R q = 2.37 ± 1.40 nm).…”

Section: Viability and Function Of Primary Human Endothelial Cells Onmentioning

confidence: 99%

Viability and function of primary human endothelial cells on smooth poly(ether imide) films

Schulz

Rüsten-Lange

Krüger

et al. 2012

Clinical Hemorheology and Microcirculation

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Poly(ether imide) (PEI) is being explored as potential biomaterial for cardiovascular applications.Different studies showed that human umbilical venous endothelial cells (HUVEC) are able to adhere and proliferate on PEI membranes (R q = 13.20 ± 1.58 nm). A recently published study revealed evidence for much lower platelet adhesion on very smooth PEI-films (R q = 2.37 ± 1.40 nm). Therefore, we explored whether primary human venous endothelial cells (HUVEC) are able to adhere and proliferate on such very smooth PEI-films compared to tissue-cultured polystyrene (TCP) as reference material.Cytotoxicity testing revealed that PEI had a slight cytotoxic effect on HUVEC accompanied by a marginal reduced integrity of the plasma membrane and a significant lower mitochondrial activity.However long-term seeding experiments up to eleven days exhibited that HUVEC were able to proliferate on the PEI-films till confluence (TCP 96,190 ± 18,289 cells/cm²; PEI 91,590 ± 19,583 cells/cm²). Further studies are planned to monitor the influence of shear force on the endothelial cell monolayer in a dynamic test system to determine its stability in view of shear resistant endothelialization of PEI for cardiovascular devices. -draft version paper - Viability and function of primary human endothelial cells on smooth poly(ether imide) filmsDipl.-Biochem. Christian Schulz Seite 2 von 21 16.08.2012 Validation of remaining solvent by headspace GCSteam sterilized PEI-film (1 g, ~150 cm²) was incubated in a sealed sample flask with 9 ml Millipore water under shaking at 37 °C for 96 hours. The supernatant atmosphere was analyzed by gas chromatography (GC) using headspace GC (HP 7694 Headspace-Sampler, HP 5890 Series II GC, DB-624, 75m column).-draft version paper - Viability and function of primary human endothelial cells on smooth poly(ether imide) filmsDipl.-Biochem. Christian Schulz Seite 4 von 21 16.08.2012 Surface characterization by contact angle and atomic force measurements (AFM)Surface contact angle measurements (DSA 100, Krüss, captive bubble, water -air) were carried out at three different areas of each sample (n = 3) analyzing the advancing (Ѳ adv. ) and receding (Ѳ rec. ) contact angle with subsequent calculating of the hysteresis.Surface topography of the PEI-films was characterized by using atomic force microscopy (AFM, NanoScope, MultiMode V, Bruker Nano GmbH, tapping mode). Prior the samples were pre-wetted in phosphate buffered saline without calcium and magnesium ions (PBS -/-) at pH 7.4. The scanning area for each scan was 50 µm square size. Surface mean roughness (R a ) and mean square roughness (R q ) were determined by analyzing three samples on three different areas. Endotoxin loadThe endotoxin load of the PEI-film (n = 4) was determined by using the Limulus amebocyte lysate (LAL) test (QCL 1000™, Lonza, Cologne, Germany). Cell culture L929-fibroblastsL929-fibroblasts (NCTC clone 929) from the connective tissue of mice were purchased from ATCC (LGC Standards GmbH, Wesel, Germany) and cultured under static cell culture co...

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Section: Viability and Function Of Primary Human Endothelial Cells Onmentioning

confidence: 99%

Viability and function of primary human endothelial cells on smooth poly(ether imide) films

Schulz

Rüsten-Lange

Krüger

et al. 2012

Clinical Hemorheology and Microcirculation

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“…PEI is non-toxic, sterilizable [1], processable to different formed-bodies [4,5], and enables a wide range of surface modifications by straightforward wet chemistry [2, 3] and was already evaluated as a biomaterial [6,24,32,35,36] suitable for applications in regenerative medicine [30,41].…”

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confidence: 99%

Interaction of thrombocytes with poly(ether imide): The influence of processing

Braune¹,

Lange²,

Richau³

et al. 2010

Clinical Hemorheology and Microcirculation

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The processing of polymers for blood contacting devices can have a major influence on surface properties. In this study, we fabricated poly(ether imide) (PEI) membranes and films to investigate the effects of the processing on physicochemical surface properties by atomic force microscopy (AFM), scanning electron microscopy, contact angle as well as zeta potential measurements. A static platelet adhesion test was performed to analyze the thrombogenicity of both devices. While contact angle measurements showed similar levels of hydrophobicity and zeta potential values were equivalent, mean surface roughness as well as surface energies in the dispersive part were found to be increased for the PEI membrane. The static platelet adhesion test showed a significantly decreased number of adherent platelets per surface area on the PEI film (178.98 ± 102.70/45000 m 2 ) compared to the PEI membrane (504 ± 314.27/45000 m 2 ) and, consequently, revealed evidence for higher thrombogenicity of the PEI membrane. This study shows that processing can have a significant effect on platelet adhesion to biomaterials, even though, molar weight was identical. Thrombogenicity of polymer-based cardiovascular devices, therefore, have to be evaluated at the final product level, following the entire processing procedure.A wide range of polymers like polyurethanes, silicones or PEG-based copolymers have received growing attention for biomedical applications due to their better hemocompatibility and processing features compared to metals [26], whereupon the ideal hemocompatible surface inhibits any platelet adhesion [20]. Several parameters seem to influence hemocompatibility. Chapman et al. concluded that hydrophilic, noncharged surfaces, without hydrogen-donors are more resistant to protein adsorption and are able to prevent platelet adherence [13]. In addition the processing itself can lead to changes e.g. in surface roughness which can influence platelet adhesion as well [16,17,31,33,42].In the present study the influence of different processing of poly(ether imide) (PEI, Ultem 1000 ® , General Electric, USA) on the interaction of blood platelets with poly(ether imide) (PEI) was analyzed. PEI is non-toxic, sterilizable [1], processable to different formed-bodies [4,5], and enables a wide range of surface modifications by straightforward wet chemistry [2, 3] and was already evaluated as a biomaterial [6,24,32,35,36] suitable for applications in regenerative medicine [30,41].Since platelet interactions are controlled by physical and chemical characteristics [26], including their possible distribution in submicroscopical domains and surface roughness [37], characteristic surface parameters were analyzed. To reveal the influences of processing on surface properties, membranes and films from PEI were fabricated and the effects on the physicochemical properties and thrombogenicity investigated. Material and methods Polymer preparationAll processed devices were fabricated from the same charge of the polymer. PEI (Ultem 1000, General Electric, USA)...

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“…A simple route to tailor PEI surface properties, for example by grafting of functional groups, allows for the adaptation of surface properties to the respective application demands (Braune et al ., ; Neffe et al ., ; Seifert et al ., ; Tzoneva et al ., ), while very good membrane‐forming properties have also been reported for PEI (Kneifel and Peinemann, ; Peinemann et al ., ). With regard to biocompatibility, PEI materials exert minimal cytotoxicity, good haemocompatibility (Imai et al ., ; Richardson et al ., ; Rüder et al ., ; Schulz et al ., ; Tzoneva et al ., ), are immunocompatible (Roch et al ., ) and allow for the attachment and growth of different cell types (Kawakami et al ., ; Kim et al ., ; Schneider et al ., ; Schulz et al ., ; Tzoneva et al ., ). On the basis of these results, PEI membranes have emerged as suitable materials for blood‐contacting applications, such as blood detoxification and oxygenation (Kawakami et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…Although PEI has shown great promise for biomedical applications through numerous in vitro studies, especially with vascular cells (Kim et al ., ; Lange et al ., ; Roch et al ., ; Schulz et al ., ; Tzoneva et al ., ) and initial in vivo biocompatibility tests of PEI bulk samples (Peluso et al ., ), the impact of basic material topography on the implant reaction has not yet been investigated. In an effort to expand the previously made observations, we investigated the biocompatibility of two basic forms of material morphology in a physiologically relevant in vivo model.…”

Section: Introductionmentioning

confidence: 99%

In vivobiocompatibility assessment of poly (ether imide) electrospun scaffolds

Haase

Krost

Sauter

et al. 2015

J Tissue Eng Regen Med

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Poly(ether imide) (PEI), which can be chemically functionalized with biologically active ligands, has emerged as a potential biomaterial for medical implants. Electrospun PEI scaffolds have shown advantageous properties, such as enhanced endothelial cell adherence, proliferation and low platelet adhesion in in vitro experiments. In this study, the in vivo behaviour of electrospun PEI scaffolds and PEI films was examined in a murine subcutaneous implantation model. Electrospun PEI scaffolds and films were surgically implanted subcutaneously in the dorsae of mice. The surrounding subcutaneous tissue response was examined via histopathological examination at 7 and 28 days after implantation. No serious adverse events were observed for both types of PEI implants. The presence of macrophages or foreign body giant cells in the vicinity of the implants and the formation of a fibrous capsule indicated a normal foreign body reaction towards PEI films and scaffolds. Capsule thickness and inflammatory infiltration cells significantly decreased for PEI scaffolds during days 7-28 while remaining unchanged for PEI films. The infiltration of cells into the implant was observed for PEI scaffolds 7 days after implantation and remained stable until 28 days of implantation. Additionally some, but not all, PEI scaffold implants induced the formation of functional blood vessels in the vicinity of the implants. Conclusively, this study demonstrates the in vivo biocompatibility of PEI implants, with favourable properties of electrospun PEI scaffolds regarding tissue integration and wound healing. Copyright © 2015 John Wiley & Sons, Ltd.

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Poly(ether imide) membranes: studies on the effect of surface modification and protein pre-adsorption on endothelial cell adhesion, growth and function

Cited by 20 publications

References 43 publications

Viability and function of primary human endothelial cells on smooth poly(ether imide) films

Viability and function of primary human endothelial cells on smooth poly(ether imide) films

Interaction of thrombocytes with poly(ether imide): The influence of processing

In vivobiocompatibility assessment of poly (ether imide) electrospun scaffolds

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