Protein immobilization on nanoporous silicon functionalized by RF activated plasma polymerization of Acrylic Acid

Rivolo, Paola; Severino, Sara Maria; Ricciardi, Serena; Frascella, Francesca; Geobaldo, Francesco

doi:10.1016/j.jcis.2013.10.060

Cited by 25 publications

(15 citation statements)

References 38 publications

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“…In the spectra of the pSi‐POct‐PAAc films (Figure (iii)), all of the above‐mentioned peaks were visible. Additionally, a sharp peak at 1722 cm −1 corresponding to the C=O vibrations of the carboxyl group of PAAc was observed . In all the samples with polymer caps, a broad peak centered at 3378 cm −1 was also evident, corresponding to the stretching vibrational mode of hydrogen bonds from absorbed water .…”

Section: Resultsmentioning

confidence: 99%

“…Additionally,as harp peak at 1722 cm À1 corresponding to the C=Ov ibrations of the carboxyl group of PAAc was observed. [17] In all the samples with polymer caps, ab road peak centered at 3378 cm À1 was also evident, corresponding to the stretching vibrational mode of hydrogen bonds from absorbed water. [16] The adsorptiono f water mostl ikely occurred immediately after the samples were removedf rom the plasma reactor andc ould not be avoided; [18] but this did not affect the performance of the plasma polymer films.…”

Section: Sem Images Inmentioning

confidence: 89%

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On‐demand Antimicrobial Treatment with Antibiotic‐Loaded Porous Silicon Capped with a pH‐Responsive Dual Plasma Polymer Barrier

Vasani

Szili

Rajeev

et al. 2017

Chemistry An Asian Journal

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Chronic wounds are am ajor socio-economic problem. Bacterial infections in such wounds are am ajor contributor to lack of wound healing. An early indicator of wound infection is an increasei np Ho ft he wound fluid. Herein, we describe the development of ap H-responsive drug delivery device that can potentially be used for wound decontamination in situ and on-demand in response to an increaseint he pH of the wound environment. The devicei sb ased on ap orouss ilicon film that provides ar eservoir for encapsulation of an antibiotic within the pores. Loaded porous silicon is capped withd ual plasma polymer layers of poly(1,7-octadiene) and poly(acrylic acid), which provide ap H-responsive barrierf or on-demand releaseo ft he antibiotic. We demonstrate that release of the antibiotic is inhibited in aqueous buffer at pH 5, whereas the drug is released in as ustainable mannera tpH8.Importantly,the released drugwas bacteriostatic against the Pseudomonas aeruginosa woundp athogen. In the future,i ncorporation of the delivery device into wound dressings could potentially be utilized for non-invasive decontamination of wounds.

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Section: Resultsmentioning

confidence: 99%

Section: Sem Images Inmentioning

confidence: 89%

On‐demand Antimicrobial Treatment with Antibiotic‐Loaded Porous Silicon Capped with a pH‐Responsive Dual Plasma Polymer Barrier

Vasani

Szili

Rajeev

et al. 2017

Chemistry An Asian Journal

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“…Different precursors and plasma excitation methods, process parameters and reactor geometries influence the plasma polymerization process and lead to a diversity of plasma-chemical reactions and thus to different film properties [58]. The low pressure plasma processes are the most widely used: microwave (2.45 GHz) [59] or capacitively coupled radio frequency (13.56 MHz) [59][60][61][62][63][64][65] activated [60], in planar parallel electrode plates geometry [63][64][65], in steel chamber [59,[63][64][65][66] or glass tubes [62,67,68] reactors, applying a continuous [61,67] or pulsed plasma discharge [59,[63][64][65]69].…”

Section: Organic Coatingsmentioning

confidence: 99%

“…This goal could be achieved through a high degree of cross-linking, but the consequent chains high fragmentation at high discharge power determinates a strong decrease of -COOH groups density. Notwithstanding, it was demonstrated that a finely tuned pulsed RF plasma discharge allows obtaining a high surface density of carboxylic groups (10 15 -10 16 -COOH/cm 2 ), stable in aqueous solutions, able to covalently graft NH 2 terminated c-DNA probes, complementary to Listeria monocytogenes oligonucleotides, for microarray diagnostics [63] and proteins (Protein A) if deposited on porous silicon matrices [64]. Moreover, when applied to polypropylene hernia-repair meshes [65], PPAAc can be successfully loaded with silver nanoparticles that were shown to be moderately released in physiological media.…”

Section: Organic Coatingsmentioning

confidence: 99%

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

et al. 2016

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Surface modification of dental implants is a key process in the production of these medical devices, and especially titanium implants used in the dental practice are commonly subjected to surface modification processes before their clinical use. A wide range of treatments, such as sand blasting, acid etching, plasma etching, plasma spray deposition, sputtering deposition and cathodic arc deposition, have been studied over the years in order to improve the performance of dental implants. Improving or accelerating the osseointegration process is usually the main goal of these surface processes, but the improvement of biocompatibility and the prevention of bacterial adhesion are also of considerable importance. In this review, we report on the research of the recent years in the field of surface treatments and coatings deposition for the improvement of dental implants performance, with a main focus on the osseointegration acceleration, the reduction of bacterial adhesion and the improvement of biocompatibility.

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“…In previous works of the authors [4,26,30,31], films with thicknesses included between 80 and 120 nm (processes performed by T Dep between 10 and 20 min) and prepared by means of both continuous wave (CW) and modulated wave (MW) plasma discharge were widely studied. The coating obtained by CW discharge were shown to not retain enough carboxylic functionalities while, among MW coatings, only the film obtained with an RF starting power of 200 W, a duty cycle of 0.1 with a t on of 10 ms and a t off of 90 ms with Ar as carrier gas, were optimal in terms of stability and reproducibility.…”

Section: Plasma Polymerized Acrylic Acidmentioning

confidence: 99%

Ultra-Thin Plasma-Polymerized Functional Coatings for Biosensing: Polyacrylic Acid, Polystyrene and Their Co-Polymer

Rivolo¹,

Castellino²,

Frascella³

et al. 2016

Crystalline and Non-Crystalline Solids

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Recently, many efforts have been done to chemically functionalize sensors surface to achieve selectivity towards diagnostics targets, such as DNA, RNA fragments and protein tumoural biomarkers, through the surface immobilization of the related specific receptor. Especially, some kind of sensors such as microcantilevers (gravimetric sensors) and one-dimensional photonics crystals (optical sensors) able to couple Bloch surface waves are very sensitive. Thus, any kind of surface modifications devoted to functionalize them has to be finely controlled in terms of mass and optical characteristics, such as refractive index, to minimize the perturbation, on the transduced signal, that can affect the response sensitivity towards the detected target species. In this work, the study and optimization of ultra-thin plasma polymers and copolymers, compatible with these constrains and obtained from the vapours of acrylic acid containing a carboxylic (−COOH) group and styrene (an aromatic molecule with a vinyl as substituent at the ring), are reported. The obtained plasma polyacrylic acid (PPAA), plasma polystyrene (PPST) and their copolymer (PPAA-ST), characterized through optical contact angle analysis (OCA), Fourier transform infrared (FTIR) spectroscopy in attenuated total reflection (ATR-FTIR), X-ray photoelectrons spectroscopy (XPS), and atomic force microscopy (AFM), are shown to match specific and critical requirements, such as low thickness (∼40 nm) and refractive index (∼1.5), high surface density of reactive groups (10 15-10 16 COOH/cm 2), bioantifouling properties where required, reproducibility, and chemical resistance and stability.

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Protein immobilization on nanoporous silicon functionalized by RF activated plasma polymerization of Acrylic Acid

Cited by 25 publications

References 38 publications

On‐demand Antimicrobial Treatment with Antibiotic‐Loaded Porous Silicon Capped with a pH‐Responsive Dual Plasma Polymer Barrier

On‐demand Antimicrobial Treatment with Antibiotic‐Loaded Porous Silicon Capped with a pH‐Responsive Dual Plasma Polymer Barrier

Surface Treatments and Functional Coatings for Biocompatibility Improvement and Bacterial Adhesion Reduction in Dental Implantology

Ultra-Thin Plasma-Polymerized Functional Coatings for Biosensing: Polyacrylic Acid, Polystyrene and Their Co-Polymer

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