Protein‐based functional nanomaterial design for bioengineering applications

Desai, Malav S.; Lee, Seung‐Wuk

doi:10.1002/wnan.1303

Cited by 46 publications

(64 citation statements)

References 235 publications

(429 reference statements)

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“…[105] Collagen has been widely used as a biomaterial in applications such as wound dressings, [106] vascular grafts, [107] neural regeneration, [108] opthamology [109] and periodontal engineering. [110] As its purification can be complicated by heterogeneities between preparations and the possibility of disease transmission, [111] its large-scale production in a recombinant platform would be advantageous.…”

Section: Expression Systems For Recombinant Productsmentioning

confidence: 99%

Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.

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Section: Expression Systems For Recombinant Productsmentioning

confidence: 99%

Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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“…Mammalian viruses have been investigated in detail for gene delivery applications (3), with numerous VLP and VNP systems undergoing clinical development and several already on the market, including the HPV vaccine Gardasil (17). Novel approaches in the development pipeline include VNPs used for theranostics and tissue engineering (190). Within the nanomedicine sector, at least one start-up company has been established focusing on the development of plant virus–based chemotherapy (Nanovector Inc.).…”

Section: Opportunities and Implicationsmentioning

confidence: 99%

Virus-Based Nanoparticles as Versatile Nanomachines

et al. 2015

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Nanoscale engineering is revolutionizing the way we prevent, detect, and treat diseases. Viruses have played a special role in these developments because they can function as prefabricated nanoscaffolds that have unique properties and are easily modified. The interiors of virus particles can encapsulate and protect sensitive compounds, while the exteriors can be altered to display large and small molecules in precisely defined arrays. These properties of viruses, along with their innate biocompatibility, have led to their development as actively targeted drug delivery systems that expand on and improve current pharmaceutical options. Viruses are naturally immunogenic, and antigens displayed on their surface have been used to create vaccines against pathogens and to break self-tolerance to initiate an immune response to dysfunctional proteins. Densely and specifically aligned imaging agents on viruses have allowed for high-resolution and noninvasive visualization tools to detect and treat diseases earlier than previously possible. These and future applications of viruses have created an exciting new field within the disciplines of both nanotechnology and medicine.

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“…To address this, we have chemically crosslinked the resultant nanofibers. NMR presents advantages over other techniques such as equilibrium swelling due to its ease of use, rapid response time and the ability to analyze crosslinking density as well as distribution when specialized forms such as multiquantum NMR is used (Desai & Lee, 2015;Lai, Yu, & Tsai, 2016). Though the crosslinking process leads to structural stability, toxicity remains a concern and the degree of However, some of the crosslinkers are toxic in nature and need to be eliminated completely before the fibers can be used for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning of tyrosine‐based oligopeptides: Self‐assembly or forced assembly?

Hamedani

Macha

Evangelista

et al. 2019

J Biomedical Materials Res

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Short oligomeric peptides typically do not exhibit the entanglements required for the formation of nanofibers via electrospinning. In this study, the synthesis of nanofibers composed of tyrosine-based dipeptides via electrospinning, has been demonstrated.The morphology, mechanical stiffness, biocompatibility, and stability under physiological conditions of such biodegradable nanofibers were characterized. The electrospun peptide nanofibers have diameters less than 100 nm and high mechanical stiffness.Raman and infrared signatures of the peptide nanofibers indicate that the electrostatic forces and solvents used in the electrospinning process lead to secondary structures different from self-assembled nanostructures composed of similar peptides. Crosslinking of the dipeptide nanofibers using 1,6-diisohexanecyanate (HMDI) improved the physiological stability, and initial biocompatibility testing with human and rat neural cell lines indicate no cytotoxicity. Such electrospun peptides open up a realm of biomaterials design with specific biochemical compositions for potential biomedical applications such as tissue repair, drug delivery, and coatings for implants.

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Protein‐based functional nanomaterial design for bioengineering applications

Cited by 46 publications

References 235 publications

Adding Functions to Biomaterial Surfaces through Protein Incorporation

Adding Functions to Biomaterial Surfaces through Protein Incorporation

Virus-Based Nanoparticles as Versatile Nanomachines

Electrospinning of tyrosine‐based oligopeptides: Self‐assembly or forced assembly?

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