Electrospinning of tyrosine‐based oligopeptides: Self‐assembly or forced assembly?

Hamedani, Yasaman; Macha, Prathyushakrishna; Evangelista, Elvira Lou; Sammeta, Vamshikrishna Reddy; Chalivendra, Vijaya; Sivappa, Rasapalli; Vasudev, Milana

doi:10.1002/jbm.a.36861

Cited by 11 publications

(18 citation statements)

References 47 publications

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“…There are several benefits in using peptides for electrospinning [ 26 ] which we should emphasize. Firstly, peptides can be specifically selected or designed to meet the criteria of biocompatibility required for biomedical applications (ranging from regenerative medicine, such as scaffolds for blood vessels, bone tissue engineering, and drug delivery) [ 25 , 30 ]. Moreover, common polymer synthetic strategies yield a distribution of molecular weights which may differ for every production [ 31 ].…”

Section: Peptides In Electrospinningmentioning

confidence: 99%

“…Focusing on short peptides and peptide-based compounds, we should emphasize that advances in peptide electrospun nanofibers have brought significant information on the forces involved in peptide assembly. Compared to “natural self-assembly”, electrospinning is based on a “forced” assembly, because the trigger for the formation of the material is the application of an electrical field [ 25 , 41 ]. It was found that those peptides which naturally self-assemble into fibers or tubes are also prone to forming electrospun fibers.…”

Section: Peptides In Electrospinningmentioning

confidence: 99%

“…More recently, Hamedani et al described the capability of tyrosine-based dipeptides to yield electrospun nanofibers of good quality (i.e., bead-free fibers without the simultaneous presence of droplets) [ 25 ]. The aim of this work was the preparation of new biodegradable and non-toxic fibers as potential biomedical candidates for tissue repair, drug delivery, or coating for implants.…”

Section: Peptides In Electrospinningmentioning

confidence: 99%

“…The use of poly- [ 21 ] and oligo-peptides [ 22 , 23 , 24 , 25 , 26 ] in electrospinning has been reported, focusing on applications in superhydrophobic surface development and rigid scaffolds for tissue engineering. Very recently, it was demonstrated that short peptides containing both natural or non-natural amino acids can be electrospun into solid, continuous, homogenous, and bead-free quasi-endless micro- and nanofibers [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

et al. 2021

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Electrospinning is a well-known, straightforward, and versatile technique, widely used for the preparation of fibers by electrifying a polymer solution. However, a high molecular weight is not essential for obtaining uniform electrospun fibers; in fact, the primary criterion to succeed is the presence of sufficient intermolecular interactions, which function similar to chain entanglements. Some small molecules able to self-assemble have been electrospun from solution into fibers and, among them, peptides containing both natural and non-natural amino acids are of particular relevance. Nowadays, the use of peptides for this purpose is at an early stage, but it is gaining more and more interest, and we are now witnessing the transition from basic research towards applications. Considering the novelty in the relevant processing, the aim of this review is to analyze the state of the art from the early 2000s on. Moreover, advantages and drawbacks in using peptides as the main or sole component for generating electrospun nanofibers will be discussed. Characterization techniques that are specifically targeted to the produced peptide fibers are presented.

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Section: Peptides In Electrospinningmentioning

confidence: 99%

Section: Peptides In Electrospinningmentioning

confidence: 99%

Section: Peptides In Electrospinningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

et al. 2021

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“…A variety of drug delivery systems have been studied so far; namely: polymeric micelles, liposomes, nanoparticles, hydrogels, bioactive membranes, and electrospun nanofibers [11][12][13][14][15][16][17]. Strategies for drug encapsulation and release can range from simple loading in a polymeric carrier and providing release through a combination of diffusion, surface hydrolysis and degradation of the matrix or a more controlled approach using stimuli responsive (pH, redox, oxidation and enzyme concentration) or remote controlled (magnetic field, temperature, light intensity and ultrasound frequency) systems [18,19].…”

Section: Introductionmentioning

confidence: 99%

Novel Honokiol-eluting PLGA-based scaffold effectively restricts the growth of renal cancer cells

et al. 2020

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Renal Cell Carcinoma (RCC) often becomes resistant to targeted therapies, and in addition, dose-dependent toxicities limit the effectiveness of therapeutic agents. Therefore, identifying novel drug delivery approaches to achieve optimal dosing of therapeutic agents can be beneficial in managing toxicities and to attain optimal therapeutic effects. Previously, we have demonstrated that Honokiol, a natural compound with potent anti-tumorigenic and anti-inflammatory effects, can induce cancer cell apoptosis and inhibit the growth of renal tumors in vivo. In cancer treatment, implant-based drug delivery systems can be used for gradual and sustained delivery of therapeutic agents like Honokiol to minimize systemic toxicity. Electrospun polymeric fibrous scaffolds are ideal candidates to be used as drug implants due to their favorable morphological properties such as high surface to volume ratio, flexibility and ease of fabrication. In this study, we fabricated Honokiol-loaded Poly(lactide-co-glycolide) (PLGA) electrospun scaffolds; and evaluated their structural characterization and biological activity. Proton nuclear magnetic resonance data proved the existence of Honokiol in the drug loaded polymeric scaffolds. The release kinetics showed that only 24% of the loaded Honokiol were released in 24hr, suggesting that sustained delivery of Honokiol is feasible. We calculated the cumulative concentration of the Honokiol released from the scaffold in 24hr; and the extent of renal cancer cell apoptosis induced with the released Honokiol is similar to an equivalent concentration of direct application of Honokiol. Also, Honokiol-loaded scaffolds placed directly in renal cell culture inhibited renal cancer cell proliferation and migration. Together, we demonstrate that Honokiol delivered through electrospun PLGA-based scaffolds is effective in inhibiting the growth of renal cancer cells; and our data necessitates further in vivo studies to explore the potential of sustained release of therapeutic agents-loaded electrospun scaffolds in the treatment of RCC and other cancer types.

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Nanoencapsulation of casein‐derived peptides within electrospun nanofibres

et al. 2021

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BACKGROUND: Bioactive peptides derived from milk proteins are recognized as functional foods, but their consumption is limited by undesirable or bitter flavour, poor stability, and low bioavailability. Electrospinning is a versatile process for encapsulation of various bioactive compounds in the form of nanosized fibres, which can circumvent these disadvantages. This study was aimed at the preparation of casein-derived peptides-loaded nanofibres through electrospinning and characterizing them for fortification of milk.RESULTS: Pullulan at 100, 120, and 140 g kg −1 concentrations was used for electrospinning of peptides. Scanning electron and atomic force micrographs revealed the formation of clean bead-free peptides-loaded pullulan nanofibres at 120 and 140 g kg −1 concentrations with mean diameter of 60.45-133.05 nm and encapsulation efficiency of 72.95-86.04%. Fourier transform infrared spectra and X-ray diffractograms revealed the absence of interactions between the functional groups of pullulan and peptides during electrospinning. The zeta potential of the peptides-loaded nanofibres ranged from −15.6 to −24.6 mV, and the hydrodynamic diameter varied from 118.7 to 256.2 nm. The peptides from electrospun nanofibres showed sustained release to the extent of 75.3% after 8 h in gastrointestinal pH conditions. The release kinetics of peptides from nanofibres was best fitted to a Peppas-Sahlin model (R 2 = 0.987), and through diffusion and erosion mechanisms. The antioxidant activity of pure peptides and those from nanofibres was comparable. The physico-chemical qualities of milk fortified with encapsulated peptides did not show noticeable difference either. CONCLUSIONS: From the morphological, ultrastructural, particle size, encapsulation efficiency, release kinetics, and antioxidant activity data, it was inferred that electrospinning could be an effective technique for nanoencapsulation of casein-derived bioactive peptides. These peptides-loaded nanofibres could be used for fortification of milk and milk products.

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Electrospinning of tyrosine‐based oligopeptides: Self‐assembly or forced assembly?

Cited by 11 publications

References 47 publications

Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

Peptide-Based Electrospun Fibers: Current Status and Emerging Developments

Novel Honokiol-eluting PLGA-based scaffold effectively restricts the growth of renal cancer cells

Nanoencapsulation of casein‐derived peptides within electrospun nanofibres

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