The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.
Rapid, sensitive,
on-site identification of SARS-CoV-2 infections
is an important tool in the control and management of COVID-19. We
have developed a surface-enhanced Raman scattering (SERS) immunoassay
for highly sensitive detection of SARS-CoV-2. Single-chain Fv (scFv)
recombinant antibody fragments that bind the SARS-CoV-2 spike protein
were isolated by biopanning a human scFv library. ScFvs were conjugated
to magnetic nanoparticles and SERS nanotags, followed by immunocomplex
formation and detection of the SARS-CoV-2 spike protein with a limit
of detection of 257 fg/mL in 30 min in viral transport medium. The
assay also detected B.1.1.7 (“alpha”), B.1.351 (“beta”),
and B.1.617.2 (“delta”) spike proteins, while no cross-reactivity
was observed with the common human coronavirus HKU1 spike protein.
Inactivated whole SARS-CoV-2 virus was detected at 4.1 × 10
4
genomes/mL, which was 10–100-fold lower than virus
loads typical of infectious individuals. The assay exhibited higher
sensitivity for SARS-CoV-2 than commercial lateral flow assays, was
compatible with viral transport media and saliva, enabled rapid pivoting
to detect new virus variants, and facilitated highly sensitive, point-of-care
diagnosis of COVID-19 in clinical and public health settings.
Natural polymers, based on proteins or polysaccharides, have attracted increasing interest in recent years due to their broad potential uses in biomedicine. The chemical stability, structural versatility, biocompatibility and high availability of these materials lend them to diverse applications in areas such as tissue engineering, drug delivery and wound healing. Biomaterials purified from animal or plant sources have also been engineered to improve their structural properties or promote interactions with surrounding cells and tissues for improved in vivo performance, leading to novel applications as implantable devices, in controlled drug release and as surface coatings. This review describes biomaterials derived from and inspired by natural proteins and polysaccharides and highlights their promise across diverse biomedical fields. We outline current therapeutic applications of these nature-based materials and consider expected future developments in identifying and utilising innovative biomaterials in new biomedical applications.
Adhesive proteins of barnacle cement have potential as environmentally friendly adhesives owing to their ability to adhere to various substrates in aqueous environments. By understanding the taxonomic breath of barnacles with different lifestyles, we may uncover commonalities in adhesives produced by these specialized organisms. The 19 kDa cement protein (cp19k) of the stalked barnacle
Pollicipes pollicipes
was expressed in
Escherichia coli
BL21 to investigate its adhesive properties. Initial expression of hexahistidine-tagged protein (rPpolcp19k-his) yielded low levels of insoluble protein. Co-overproduction of
E. coli
molecular chaperones GroEL-GroES and trigger factor (TF) increased soluble protein yields, although TF co-purified with the target protein (TF-rPpolcp19k-his). Surface coat analysis revealed high levels of adsorption of the TF-rPpolcp19k-his complex and of purified
E. coli
TF on both hydrophobic and hydrophilic surfaces, while low levels of adsorption were observed for rPpolcp19k-his. Tag-free rPpolcp19k protein also exhibited low adsorption compared to fibrinogen and Cell-Tak controls on hydrophobic, neutral hydrophilic and charged self-assembled monolayers under surface plasmon resonance assay conditions designed to mimic the barnacle cement gland or seawater. Because rPpolcp19k protein displays low adhesive capability, this protein is suggested to confer the ability to self-assemble into a plaque within the barnacle cement complex.
This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’.
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