Protein and lipid requirements of three-spot cichlid Cichlasoma trimaculatum larvae

Toledo-Solís, Francisco Javier; Martínez‐García, Rafael; Galavíz, Mario A.; Hilerio-Ruiz, Andrea Guadalupe; Álvarez‐González, Carlos Alfonso; Rodrigáñez, M. Sáenz de

doi:10.1007/s10695-019-00692-9

Cited by 10 publications

(6 citation statements)

References 80 publications

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“…However, in biochemical aspects, we can also observe a similarity in the pattern of the genetic expression of these digestive enzymes such as trypsin and chymotrypsin with the enzymatic activities in the same species (López‐Ramírez et al, 2011) and C. trimaculatum (Hilerio‐Ruíz et al, 2021), where a comparative increase in the gene expression and their enzymatic activity is observed. For his part (Karp, 2009), he mentions that there must first be an expression of the proenzyme (zymogen) so that there can be an enzymatic activity, therefore, the results obtained in this work show that the digestion process is carried out, in the first instance, by the enzymes of pancreatic origin as in other previously carried out studies (Toledo‐Solis et al, 2020). In both cases, almost zero expression and enzymatic activity is observed in the first DAH being, from day 6, when the expression is detected (trypsinogen) and in parallel the elevation of the enzymatic activity (trypsin), all of them coinciding with the beginning of exogenous feeding ( Artemia nauplii).…”

Section: Discussionsupporting

confidence: 53%

Molecular expression of digestive enzymes during the ontogeny of the Mayan cichlid ( Cichlasoma urophthalmus )

Frías‐Quintana

Jiménez‐Martínez

Álvarez‐González

et al. 2021

Aquaculture Research

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The Mayan cichlid (Cichlasoma urophthalmus), is a freshwater fish of the Cichlidae family, native to the south east of Mexico, it has potential for extensive cultivation due to its excellent meat quality and local demand. However, the nutrigenomic aspects and molecular expression of genes involved in its digestion that could contribute to its growth during the larval stage are unknown. Some enzymes such as Pepsin (PEP), pancreatic such as trypsin (TRY), Chymotrypsin [CHY] and intestinal enzymes such as Leucine aminopeptidase [ANPEP], function during larval ontogenesis and activity patterns of the key factors involved in the digestive system. For this study, 30–50 larvae were collected, which were processed with mRNA extraction techniques and cDNA synthesis by RT‐PCR using qPCR oligos designed from Oreochromis niloticus, measuring molecular expression during the initial ontogeny of C. urophthalmus. The results obtained show the molecular expression of these enzymes occurs through epigenetic factors such as changes from endogenous to exogenous feeding, presenting the activation of genes for the formation of organs of the digestive tract that allow the secretion of specific enzymes for processing and digestion of food, that can activate these genes to increase the digestion efficiency and the optimal use of nutrients.

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Section: Discussionsupporting

confidence: 53%

Molecular expression of digestive enzymes during the ontogeny of the Mayan cichlid ( Cichlasoma urophthalmus )

Frías‐Quintana

Jiménez‐Martínez

Álvarez‐González

et al. 2021

Aquaculture Research

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“…(2018) highlighted the importance of understanding social behaviour in order to propose effective measures to manipulate biotic and abiotic factors to reduce aggression and mortality in larval stages. Another factor to be considered may be nutrition, and it is important to search for diets that meet the requirements of larvae in order to avoid this problem (Toledo‐Solís et al., 2020), since the tested densities did not affect survival.…”

Section: Discussionmentioning

confidence: 99%

Stocking density in Colossoma macropomum larviculture, a freshwater fish, in recirculating aquaculture system

2020

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This study aimed to investigate stocking density during larviculture of Colossoma macropomum in recirculating aquaculture system with slightly saline water. Densities of 10 (D10), 30 (D30) and 50 (D50) larvae/L were tested with five replicates each. Larvae were fed with Artemia nauplii during the first 15 days and with commercial diet from day 16 to day 30. Water was maintained at a salinity of 2.01 ± 0.41 g of salt/L, and performance and survival were evaluated throughout the experiment. At the end of the experiment, larvae of each tank were classified according to size as small—S (<18.0 mm and <0.08 g), medium—M (>18.0 mm and <22.2 mm, >0.08 g and <0.15 g) and large—L (>22.2 mm and >0.15 g), and the size distribution was calculated for each stocking densities evaluated. Larval weight, length and specific growth rate were not affected by the tested stocking densities throughout the experiment (p > .05). Heterogeneity was recorded at 30 days of larviculture for all tested densities with a predominance of class M animals. Survival during feeding with Artemia was not affected by the tested densities (p > .05). Survival during feeding with commercial diet was highest for D10 and lowest for D30 (p < .05). Global survival, considering the experiment as a whole, was similar among the tested stocking densities (p > .05). Larviculture of C. macropomum can be successfully carried out at stocking densities of up to 50 larvae/L during the first 30 days of rearing in slightly saline water in recirculating aquaculture system.

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“…On the whole, α‐amylase and proteolytic enzyme activities of fish fed with diets containing low levels of protein were found to be initially low, which increased up to a certain point with increased protein levels and then decreased sharply. Such reduction in proteolytic enzyme activities of fish fed high protein levels might arise from the inhibition or inactivation of the enzyme using autohydrolysis of excess nutrients (Lazo et al, 2011; Toledo‐Solís et al, 2020; Yúfera et al, 2018; Zambonino‐Infante & Cahu, 2001). However, this does not explain the trend in α‐amylase activity because as the protein ratio in the feed increased, the starch ratio decreased.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary protein level on growth, histology and digestive enzyme activities of ornamental fish Ancistrus cirrhosus

Şahin

Gürkan

2022

Aquaculture Research

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Regardless of the developmental stage of the cultured organism, proper nutritional care is essential. A significant number of aquacultural species rely on protein as their main energy source and protein feeds sold in the ornamental market are formulated with widely varying protein inclusion levels. As Ancistrus cirrhosus is one of the most popular ornamental fish species, this study aimed to determine the effects of different protein levels on growth, digestive enzyme activities and liver and intestinal histopathology. Therefore, 450 fry (98.5 ± 3.1 mg [mean ± SE]), 5-6 weeks old, were fed six different experimental diets containing 30%, 35%, 40%, 45%, 50% and 55% crude protein in triplicate groups for 60 days. The results demonstrated that no significant differences were observed in the final lengths, whole-body protein levels, body condition indices and lipase and alkaline phosphatase activities of fish between groups. The fish fed with a 40% protein diet differed positively from other experimental groups in terms of final weight, FCR, SGR, proteolytic enzyme activities and histopathological findings. Lower proteolytic activity was ascertained in fish fed feed containing lower levels of protein (30%, 35%) and increased histopathological defects in the intestine and liver of fish fed feed containing over 40% protein (45%, 50% and 55%). Based on our results, a diet with a 40% protein level is most beneficial for the rearing of A. cirrhosus fry. Further research is required to determine optimal ratios, which are predicted to be between 35 and 40.

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Protein and lipid requirements of three-spot cichlid Cichlasoma trimaculatum larvae

Cited by 10 publications

References 80 publications

Molecular expression of digestive enzymes during the ontogeny of the Mayan cichlid ( Cichlasoma urophthalmus )

Molecular expression of digestive enzymes during the ontogeny of the Mayan cichlid ( Cichlasoma urophthalmus )

Stocking density in Colossoma macropomum larviculture, a freshwater fish, in recirculating aquaculture system

Effects of dietary protein level on growth, histology and digestive enzyme activities of ornamental fish Ancistrus cirrhosus

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