Protein adsorption on polymeric biomaterials I. Adsorption isotherms

Young, Brian R.; Pitt, William G.; Cooper, Stuart L.

doi:10.1016/0021-9797(88)90321-9

Cited by 166 publications

(79 citation statements)

References 26 publications

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“…Gelatin is the partially hydrolyzed form of collagen, the crucial component of the extra cellular matrix. Its ability to increase cell attachment has been previously described (Young et al, 1988). We report the use of agar with gelatin in the construction of tissue engineering scaffolds.…”

Section: Introductionmentioning

confidence: 90%

Fabrication of agar‐gelatin hybrid scaffolds using a novel entrapment method for in vitro tissue engineering applications

Verma

Kar

et al. 2006

Biotech & Bioengineering

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Scaffolds of agar and gelatin were developed using a novel entrapment method where agar and gelatin molecules mutually entrapped one another forming stable cell adhesive matrices. Glutaraldehyde was used as a crosslinking agent for gelatin. Three types of hybrid matrices were prepared using agar and gelatin in different proportions in the weight ratio of 1:1, 2:1, and 3:1. Surface characterization of dry scaffolds was carried out by scanning electron microscope. Swelling studies were carried out in phosphate buffer saline (PBS) at physiological pH 7.4. The integral stability of the scaffolds was evaluated by estimating the released disintegrated gelatin from them in PBS at pH 7.4. The attachment kinetics of the cells was evaluated by culturing mouse fibroblast cell line NIH 3T3 on films. The cytocompatibility of these matrices was determined by studying growth kinetics of NIH 3T3 cells on them and morphology of cells was observed through optical photographs taken at various days of culture. It was found that the matrices containing agar and gelatin in 2:1 weight ratio exhibited best growth kinetics. The results obtained from these studies have suggested that the above-described method is a cheap and easy way to fabricate agar-gelatin hybrid scaffolds to grow cells which can be used in various in vitro tissue engineering applications like screening of drugs.

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Section: Introductionmentioning

confidence: 90%

Fabrication of agar‐gelatin hybrid scaffolds using a novel entrapment method for in vitro tissue engineering applications

Verma

Kar

et al. 2006

Biotech & Bioengineering

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“…[152,153] Conversely, MFNs made from hard materials, such as Si, glass, or plastics, can be used to pattern PDMS surfaces. The adsorption of protein from solution to PDMS is, like on many other hydrophobic surfaces, rapid, spontaneous, and largely irreversible, [154][155][156] which alleviates the need to treat PDMS surfaces for binding protein molecules. [157] Two hard surfaces in contact may not conform and have asperities that can cause a liquid to leak outside the region of a surface where confinement is sought.…”

Section: Sealing a Microfluidic Device With A Surfacementioning

confidence: 99%

Microfluidics for Processing Surfaces and Miniaturizing Biological Assays

2005

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This review is an account of our efforts to develop a versatile and flexible microfluidic technology for surface‐processing applications and miniaturizing biological assays. The review is presented in the context of current trends in microfluidic technology and addresses some of the major challenges for confining chemical and biochemical processes on surfaces: the sealing of a microchannel with a surface, the world‐to‐chip interface, the displacement of liquids in small conduits, the sequential delivery of multiple solutions, the accurate patterning of surfaces, the coincident detection of various analytes, and the detection of analytes in a small and dilute sample. Our solutions to these problems include the use of reversible sealing, capillary phenomena for powering and controlling liquid transport, and non‐contact microfluidics for spotting and drawing (on surfaces) with flow conditions. These solutions offer many advantages over conventional techniques for handling minute amounts of liquids and may find applications in lithography, biopatterning (e.g., the patterning of biomolecules), diagnostics, drug discovery, and also cellular assays.

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“…46 The measured surface densities are reported in Table I. Monolayer coverage may range between 0.21 g/cm 2 for side-on absorption to 1.57 g/cm 2 for end-on adsorption; 38 therefore, the adsorbed fibrinogen density for each material falls within the monolayer range.…”

Section: Disk Preparationmentioning

confidence: 99%

Quantitative comparison of shear-dependentStaphylococcus aureus adhesion to three polyurethane ionomer analogs with distinct surface properties

Dickinson

Nagel

Proctor

et al. 1997

J. Biomed. Mater. Res.

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Bacterial adhesion is a central step in infection on biomaterial surfaces; however, the relation between biomaterial surface properties and adhesion remains poorly understood. To quantitatively determine the relationship among polyurethane surface properties, protein coating, and adhesion, we have compared attachment and detachment kinetics of Staphylococcus aureus on three different novel polyurethanes with different protein coatings. Rate constants for attachment or detachment were measured as a function of shear rate in a well-defined laminar flow field. The tested polyurethanes included a relatively hydrophobic-base polyether urethane and hydrophilic anionomer and cationomer analogs of the base material. Materials were tested bare, or coated with human fibrinogen, plasma, or albumin. The results suggest that the presence of fibrinogen or plasma greatly enhance the attachment rate constants and decrease the detachment rate constants on all materials. The most extreme differences among the different materials were observed on the bare materials, with the base polyurethane being most resistant to both attachment and detachment. However, except for a reduced attachment rate constant on the plasma-coated sulfonated polyurethane, few differences in the rate constants were observed among protein-coated materials, suggesting the primary role of surface properties is masked by the presence of the adsorbed protein layer.

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Protein adsorption on polymeric biomaterials I. Adsorption isotherms

Cited by 166 publications

References 26 publications

Fabrication of agar‐gelatin hybrid scaffolds using a novel entrapment method for in vitro tissue engineering applications

Fabrication of agar‐gelatin hybrid scaffolds using a novel entrapment method for in vitro tissue engineering applications

Microfluidics for Processing Surfaces and Miniaturizing Biological Assays

Quantitative comparison of shear-dependentStaphylococcus aureus adhesion to three polyurethane ionomer analogs with distinct surface properties

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