Protective role of exogenous recombinant peroxiredoxin 6 under ischemia-reperfusion injury of kidney

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

“…The findings of the present study coincide with the data on the nephroprotective role of another representative of the peroxiredoxin family—Prx6 in the protection of the kidneys in I/R injury. Interestingly, Prx1 and Prx6 are comparable in efficiency, despite the significantly lower peroxidase activity of Prx6, which can be explained by different substrate specificity of these enzymes, i.e., notwithstanding the lower peroxidase activity, Prx6 is able to neutralize peroxide substrates that cannon be eliminated by Prx1, for example, phospholipid peroxides [ 16 ]. The combined use of Prx1 and Prx6 may be a more promising approach in preventing the after-effects of I/R injuries and preserving ischemic organs during transplantation.…”

“…It must be noted that some Prx representatives have been investigated previously in models of artificially induced oxidative stress: total irradiation, renal ischemia-reperfusion injury, small intestine, etc. [ 13 , 14 , 15 , 16 , 17 ]. It is the Prx family proteins that have shown high efficiency in oxidative stress neutralization, which is associated with the high antioxidant activity of these enzymes and a wide range of neutralizable hydroperoxides, both organic and inorganic in nature.…”

Peroxiredoxin 6 Attenuates Alloxan-Induced Type 1 Diabetes Mellitus in Mice and Cytokine-Induced Cytotoxicity in RIN-m5F Beta Cells

“…Thus, we obtained evidence that the protective effects of PRDX6 in T1D are related to its antioxidant activity. Interestingly, using another model of oxidative stress in vivo, we showed that the preliminary PRDX6 treatment reduced the level of malonic aldehyde in the ischemia-reperfusion kidney [22].…”

“…Genetic constructions encoding (human) PRDX6 enzymes were obtained and expressed earlier in E. coli BL21(DE3) cells [ 22 ]. Recombinant proteins harbored His-tag, so the enzymes were purified by affinity chromatography on Ni-NTA-agarose (Thermo Fisher Scientific, USA), according to the manufacturer's recommendations.…”

“…Control mice received intravenously 0.1 mL of physiological saline. Previously, we found that after intravenous administration, PRDX6 retained the highest level in the blood for 10 minutes; then, its amount gradually decreased, but after 6 hours, about 30% of the administered PRDX6 remained in blood plasma [22]. So, it was proved that possible PRDX6 effects were caused by its presence in the animal tissues.…”