Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

Шарапов, М. Г.; Гончаров, Р. Г.; Filkov, Gleb I.; Trofimenko, A. V.; Boyarintsev, V. V.; Новоселов, В. И.

doi:10.3390/antiox9080680

Cited by 8 publications

(3 citation statements)

References 96 publications

(117 reference statements)

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“…The expression of this gene was more than 2-fold higher in A549 than in H1975 cells, albeit this difference was not significant. PRDX2 (an isoform more sensitive to peroxidation than PRDX1 [ 50 ]) was present in H1975 cells at low levels, these levels being even lower in A549 cells under our experimental conditions. The A549 cell line also has a lower level of GPX1 than the H1975 cells, this difference being significant ( p < 0.001).…”

Section: Resultsmentioning

confidence: 87%

MnTnHex-2-PyP5+ Displays Anticancer Properties and Enhances Cisplatin Effects in Non-Small Cell Lung Cancer Cells

et al. 2022

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The manganese(III) porphyrin MnTnHex-2-PyP5+ (MnTnHex) is a potent superoxide dismutase mimic and modulator of redox-based transcriptional activity that has been studied in the context of different human disease models, including cancer. Nevertheless, for lung cancer, hardly any information is available. Thus, the present work aims to fill this gap and reports the effects of MnTnHex in non-small cell lung cancer (NSCLC) cells, more specifically, A549 and H1975 cells, in vitro. Both cell lines were initially characterized in terms of innate levels of catalase, glutathione peroxidase 1, and peroxiredoxins 1 and 2. To assess the effect of MnTnHex in NSCLC, alone or in combination with cisplatin, endpoints related to the cell viability, cell cycle distribution, cell motility, and characterization of the volatile carbonyl compounds (VCCs) generated in the extracellular medium (i.e., exometabolome) were addressed. The results show that MnTnHex as a single drug markedly reduced the viability of both NSCLC cell lines, with some IC50 values reaching sub-micromolar levels. This redox-active drug also altered the cell cycle distribution, induced cell death, and increased the cytotoxicity pattern of cisplatin. MnTnHex also reduced collective cell migration. Finally, the metabolomics study revealed an increase in the levels of a few VCCs associated with oxidative stress in MnTnHex-treated cells. Altogether these results suggest the therapeutic potential of MnTnHex to be further explored, either alone or in combination therapy with cisplatin, in NSCLC.

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Section: Resultsmentioning

confidence: 87%

MnTnHex-2-PyP5+ Displays Anticancer Properties and Enhances Cisplatin Effects in Non-Small Cell Lung Cancer Cells

et al. 2022

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“…В настоящее время существует ряд нерешенных проблем в работе направления трансплантации и пер фузии донорских легких -ишемическиреперфузи онное повреждение, отсутствие высокоэффективной антиоксидантной защиты, отсутствие дешевой и лег ко воспроизводимой экспериментальной модели для научных исследований, результаты которых можно экстраполировать на человека [1][2][3]. Животные явля ются предпочтительной экспериментальной моделью для исследования дыхательной системы, аналогич ной человеческой.…”

Section: Introductionunclassified

Development of an experimental technique for orthotopic left lung transplantation in a rabbit model

Bogdanov,

Pashkov,

Yakunin

et al. 2024

RJTAO

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Objective: to develop, master and evaluate the efficiency of an isolated lung transplantation (LT) technique on a rabbit animal model using Perfadex Plus® solution for cold static storage. Materials and methods. Scottish Giant rabbits (n = 20) were used in this study and divided into two groups: donors and recipients. Donor lungs were preserved with Perfadex Plus® solution and stored for 6 hours at 4 °C. Recipient animals underwent unilateral orthotopic left LT. The postoperative follow-up period was 24 hours. Laboratory and instrumental control with assessment of blood gas composition, lactate level, ventilation parameters, and central hemodynamic parameters, was performed during the follow-up. Chest X-ray in direct projection was performed twice, and at the end of follow-up, material was taken for histologic examination. Results. We obtained a high oxygenation index in the post-transplant period (>350 at p < 0.023), as well as physiological indicators of lactate (3 ± 0.3 mmol/L at p < 0.002) and peak inspiratory pressure (15 ± 1 cmH2O, p < 0.001). Radiological examination showed no radiological signs of severe primary graft dysfunction in all cases (mean RALE score 1), which was confirmed by histological studies. Conclusion. Left LT in rabbits is possible, the LT technique on a biological rabbit model using Perfadex Plus® solution is valid and efficient with the achievement of satisfactory gas exchange, ventilation and metabolism parameters.

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“…Therefore, OS can accelerate kidney disease progression. Different PRDX isoforms were reported to be involved in diabetic nephropathy (Lee and Lee 2018), ischemia/reperfusion damage (Sharapov et al 2020), obstructive kidney disease (Hwang et al 2019), ciliopathies (Zacchia et al 2020) and acute tubular necrosis (Wu et al 2017). However, the role of PRDXs in the pathophysiology of glomerular diseases is not well known.…”

Section: Introductionmentioning

confidence: 99%

Peroxiredoxins as Markers of Oxidative Stress in IgA Nephropathy, Membranous Nephropathy and Lupus Nephritis

Krata

Foroncewicz

Zagożdżon

et al. 2021

Arch. Immunol. Ther. Exp.

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IgA nephropathy (IgAN), membranous nephropathy (MN), and lupus nephritis (LN) represent important causes of chronic kidney disease. They belong to the immune-mediated glomerulonephritis (GNs), and have distinct pathogenesis, distinct clinical courses, and variable responses to treatment. Therefore, specific diagnostic procedures are necessary for more effective patient management. Recently, a role for oxidative stress has been proposed in various renal disorders. Thus, molecules related to oxidative stress, such as 2-Cys-peroxiredoxins (PRDXs), may represent plausible candidates for biomarkers in renal pathologies. The aim of this study was to assess whether there are differences between individual GNs and healthy controls in the context of PRDXs serum concentration. We enrolled 108 patients with biopsy-proven IgAN (47), MN (26), LN (35) and 30 healthy age- and sex-matched controls. The serum concentrations of PRDX 1–5 were measured with ELISA assays and correlated with demographic and clinical data. The PRDXs’ concentration varied depending on the GN type. We also observed an association of PRDXs with lower estimated glomerular filtration rates, complement, hemoglobin, and body mass index. Our study indicates that individual PRDX can play roles in pathophysiology of selected GNs and that their serum concentrations may become useful as a new supplementary diagnostic markers in IgAN, MN as well as LN. The results of this study open a new avenue for prospective research on PRDXs in renal diseases.

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Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

Cited by 8 publications

References 96 publications

MnTnHex-2-PyP5+ Displays Anticancer Properties and Enhances Cisplatin Effects in Non-Small Cell Lung Cancer Cells

MnTnHex-2-PyP5+ Displays Anticancer Properties and Enhances Cisplatin Effects in Non-Small Cell Lung Cancer Cells

Development of an experimental technique for orthotopic left lung transplantation in a rabbit model

Peroxiredoxins as Markers of Oxidative Stress in IgA Nephropathy, Membranous Nephropathy and Lupus Nephritis

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