A chimeric recombinant protein with peroxidase and superoxide dismutase activities: Physico-chemical characterization and applicability to neutralize oxidative stress caused by ionizing radiation

Шарапов, М. Г.; Новоселов, В. И.; Samygina, V.R.; Konarev, Petr V.; Молочков, А. В.; Sekirin, A.; Балканов, А. С.; Gudkov, Sergey V.

doi:10.1016/j.bej.2020.107603

Cited by 7 publications

(6 citation statements)

References 57 publications

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“…Recombinant proteins contain a His-tag at the carboxyl terminus; therefore, the enzymes were purified under consistent conditions using affinity chromatography on Ni-NTA agarose (Thermo Fisher Scientific, Hilden, Germany), in accordance with the manufacturer′s recommendations. The method for protein extraction and purification is described previously [ 45 ]. The purity of the obtained enzymes was not less than 95%, as judged by electrophoresis in 10% SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

“…Erythrocytes were removed by centrifugation (1500× g , NT). The resulting plasma was 10-fold diluted with buffer (1xPBS, 10 mM imidazole, pH 7.4) for application to a Ni-NTA agarose column and the protein was purified according to [ 45 ]. After that, the residual peroxidase activity of Prx1 and Prx2 was assessed according to the previously described technique [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

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Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

et al. 2020

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The pathogenesis of ischemia-reperfusion (I/R) injuries is based on oxidative stress caused by a sharp increase in the concentration of free radicals, reactive oxygen species (ROS) and secondary products of free radical oxidation of biological macromolecules during reperfusion. Application of exogenous antioxidants lowers the level of ROS in the affected tissues, suppresses or adjusts the course of oxidative stress, thereby substantially reducing the severity of I/R injury. We believe that the use of antioxidant enzymes may be the most promising line of effort since they possess higher efficiency than low molecular weight antioxidants. Among antioxidant enzymes, of great interest are peroxiredoxins (Prx1–6) which reduce a wide range of organic and inorganic peroxide substrates. In an animal model of bilateral I/R injury of kidneys (using histological, biochemical, and molecular biological methods) it was shown that intravenous administration of recombinant typical 2-Cys peroxiredoxins (Prx1 and Prx2) effectively reduces the severity of I/R damage, contributing to the normalization of the structural and functional state of the kidneys and an almost 2-fold increase in the survival of experimental animals. The use of recombinant Prx1 or Prx2 can be an efficient approach for the prevention and treatment of renal I/R injury.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

et al. 2020

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“…For example, most injectable medications are small molecule or cytogenic pharmaceuticals, and they are among the most extensively utilized dose types to alleviate radiationinduced intestine injury. [9,87,88] However, high doses of the injectable medication may instead cause intestine injuries, which can be avoided by delivery based on inorganic drug carriers due to their advantages of stability, high loading capacity, and longer circulation stay in the body. For example, silica, a commonly used nano carrier with low toxicity and biodegradability, was adopted for the delivery of melanin by Schweitzer et al to synthesize the melanin-covered nanoparticles.…”

Section: Small Molecule Polymerization Of Radioprotective Drugsmentioning

confidence: 99%

“…Immediately afterward, the IR-induced ROS further destroys the biological molecules and cellular structure, leading to indirect destructive effects, eventually resulting in cell dysfunction, catastrophe, and even death. [7][8][9] Excessive ROS is preferable to kill tumor cells and repress cancer progression via direct apoptosis and activated immune responses, [10][11][12][13][14][15][16][17] but for normal tissues or organs, it is a hazard. [18] With arising from the primary damages mentioned above, a variety of radiation diseases sprouts gradually.…”

Section: Introductionmentioning

confidence: 99%

Nanodrugs with intrinsic radioprotective exertion: Turning the double‐edged sword into a single‐edged knife

Guo¹,

Zhao

Shang

et al. 2023

Exploration

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Ionizing radiation (IR) poses a growing threat to human health, and thus ideal radioprotectors with high efficacy and low toxicity still receive widespread attention in radiation medicine. Despite significant progress made in conventional radioprotectants, high toxicity, and low bioavailability still discourage their application. Fortunately, the rapidly evolving nanomaterial technology furnishes reliable tools to address these bottlenecks, opening up the cutting‐edge nano‐radioprotective medicine, among which the intrinsic nano‐radioprotectants characterized by high efficacy, low toxicity, and prolonged blood retention duration, represent the most extensively studied class in this area. Herein, we made the systematic review on this topic, and discussed more specific types of radioprotective nanomaterials and more general clusters of the extensive nano‐radioprotectants. In this review, we mainly focused on the development, design innovations, applications, challenges, and prospects of the intrinsic antiradiation nanomedicines, and presented a comprehensive overview, in‐depth analysis as well as an updated understanding of the latest advances in this topic. We hope that this review will promote the interdisciplinarity across radiation medicine and nanotechnology and stimulate further valuable studies in this promising field.

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“…Results were calculated according to a standard method. All details have been previously described [53].…”

Section: Gene Expressionmentioning

confidence: 99%

Selenium Nanoparticles Can Influence the Immune Response Due to Interactions with Antibodies and Modulation of the Physiological State of Granulocytes

et al. 2022

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Currently, selenium nanoparticles (SeNPs) are considered potential immunomodulatory agents and as targets for activity modulation are granulocytes, which have the most abundant population of immune blood cells. The present study aims to evaluate the cytotoxic effect and its effect on the functional responses of granulocytes. In addition to the intrinsic activity of SeNPs, we studied the activity of the combination of SeNPs and IgG antibodies. Using laser ablation and fragmentation, we obtained nanoparticles with an average size of 100 nm and a rather narrow size evolution. The resulting nanoparticles do not show acute toxicity to primary cultures of fibroblasts and hepatocytes, epithelial-like cell line L-929 and granulocyte-like culture of HL-60 at a concentration of 109 NPs/mL. SeNPs at a concentration of 1010 NPs/mL reduced the viability of HL-60 cells by no more than 10% and did not affect the viability of the primary culture of mouse granulocytes, and did not have a genotoxic effect on progenitor cells. The addition of SeNPs can affect the production of reactive oxygen species (ROS) by mouse bone marrow granulocytes, modulate the proportion of granulocytes with calcium spikes and enhance fMLF-induced granulocytes degranulation. SeNPs can modulate the effect of IgG on the physiological responses of granulocytes. We studied the expression level of genes associated with inflammation and cell stress. SeNPs increase the expression of catalase, NF-κB, Xrcc5 and some others; antibodies enhance the effect of SeNPs, but IgG without SeNPs decreases the expression level of these genes. This fact can be explained by the interaction between SeNPs and IgG. It has been established that antibodies interact with SeNPs. We showed that antibodies bind to the surface of selenium nanoparticles and are present in aqueous solutions in a bound form from DLS methods, ultraviolet–visible spectroscopy, vibrational–rotational spectrometry, fluorescence spectrometry, and refractometry. At the same time, in a significant part of the antibodies, a partial change in the tertiary and secondary structure is observed. The data obtained will allow a better understanding of the principles of the interaction of immune cells with antibodies and SeNPs and, in the future, may serve to create a new generation of immunomodulators.

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A chimeric recombinant protein with peroxidase and superoxide dismutase activities: Physico-chemical characterization and applicability to neutralize oxidative stress caused by ionizing radiation

Cited by 7 publications

References 57 publications

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

Nanodrugs with intrinsic radioprotective exertion: Turning the double‐edged sword into a single‐edged knife

Selenium Nanoparticles Can Influence the Immune Response Due to Interactions with Antibodies and Modulation of the Physiological State of Granulocytes

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