Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination

Lorenzen, Niels; Lorenzen, Ellen; Einer-Jensen, Katja; Heppell, Joël; Wu, Tao; Davis, Heather L.

doi:10.1006/fsim.1997.0134

Cited by 183 publications

(113 citation statements)

References 14 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…E-mail: gael-kurath@usgs.gov many species against various pathogens. In salmon and trout, the glycoprotein genes of pathogenic rhabdoviruses, including IHNV and viral haemorragic septicemia virus (VHSV) (Anderson et al 199613, Boudinot et al 1998, Lorenzen et al 1998, have been shown to be protective when used in a DNA vaccine. Other gene products, however, have been given little attention because most vaccines aim at presenting surface proteins to the host immune system.…”

Section: Infectious Hematopoietic Necrosis Virus (Ihnv) Is the Most Imentioning

confidence: 99%

“…Furthermore, Lorenzen et al (1998) demonstrated in rainbow trout that a DNA vaccine containing only the nucleocapsid (N) gene of VHSV was able to induce some level of protective immunity against viral challenge. In contrast, a DNA vaccine encoding the N gene of IHNV was shown not to induce antibody response or protection in rainbow trout against viral challenge (Anderson et al 1996b).…”

Section: Infectious Hematopoietic Necrosis Virus (Ihnv) Is the Most Imentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines

Corbeil¹,

LaPatra²,

Anderson³

et al. 1999

Dis. Aquat. Org.

113

View full text Add to dashboard Cite

Section: Infectious Hematopoietic Necrosis Virus (Ihnv) Is the Most Imentioning

confidence: 99%

Section: Infectious Hematopoietic Necrosis Virus (Ihnv) Is the Most Imentioning

confidence: 99%

Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines

Corbeil¹,

LaPatra²,

Anderson³

et al. 1999

Dis. Aquat. Org.

113

View full text Add to dashboard Cite

“…The VHSV DNA vaccine used in this study, designated pVHSV, has been previously described [15,23]. The empty pcDNA3 plasmid was used as a control.…”

Section: Vhsv Dna Vaccinementioning

confidence: 99%

“…DNA vaccination has proved as an effective method for inducing a potent and long-lasting protection against different fish viruses, especially rhabdoviruses such as infectious hematopoietic necrosis virus (IHNV) or viral haemorrhagic septicaemia virus (VHSV) [1][2][3][4]. Significant protection levels have also been achieved with DNA vaccines constructed for other fish viruses [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Castro¹,

Martinez-Alonso²,

Fischer³

et al. 2014

Vaccine

View full text Add to dashboard Cite

a b s t r a c tIn fish, intramuscular (i.m) injection of plasmid DNA encoding viral proteins has proved a highly effective vaccination strategy against some viral pathogens. The efficacy of DNA vaccination in teleost fish is based on the high level of viral antigen expression in muscle cells inducing a strong and long-lasting protection. However, the mechanisms through which this protection is established and effectuated in fish are still not fully understood. Moreover, similarities to mammalian models cannot be established since DNA vaccination in mammals usually induces much weaker responses. In this work, we have focused on the characterization of the immune cells that infiltrate the muscle at the site of DNA injection in vaccinated fish and the chemokines and chemokine receptors that may be involved in their infiltration. We have demonstrated through diverse techniques that B lymphocytes, both IgM + and IgT + cells, represented a major infiltrating cell type in fish vaccinated with a viral haemorrhagic septicaemia virus (VHSV) glycoprotein-encoding DNA vaccine, whereas in control fish injected with an oil adjuvant mainly granulocyte/monocyte-type cells were attracted. Among twelve chemokine genes studied, only CXCL11 L1, CK5B and CK6 mRNA levels were up-regulated in DNA vaccinated fish compared to fish injected with the corresponding vector backbone. Furthermore, the transcription of CXCR3B, a possible receptor for CXCL11 L1 was also significantly up-regulated in vaccinated fish. Finally, experiments performed with recombinant trout CK5B and CK6 and chemokine expression plasmids revealed that these chemokines have chemotactic capacities which might explain the recruitment of B cells to the site of DNA injection. Altogether, our results reveal that there is an early chemokine-related B cell recruitment triggered by i.m. DNA vaccination against VHSV which might play an important role in the initial phase of the immune response.

show abstract

“…Thus, the first fish DNA vaccines against IHNV were reported in 1996 (Anderson et al, 1996a;Anderson et al, 1996b) and against viral haemorrhagic septicemia virus (VHSV) in 1998 (Lorenzen et al, 1998). In 2005, Vical-Aqua Health Ltd. of Canada (Novartis APEXwww.intechopen.com IHN) received authorization to commertialize an IHNV DNA vaccine (Salonius et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Encinas¹,

Gómez-Casado²,

Estepa³

et al. 2012

Health and Environment in Aquaculture

View full text Add to dashboard Cite

Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination

Cited by 183 publications

References 14 publications

Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines

Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Contact Info

Product

Resources

About