Protective Effects of the Nuclear Factor Kappa B Inhibitor Pyrrolidine Dithiocarbamate on Experimental Testicular Torsion and Detorsion Injury

Abstract

Testicular torsion results with the damage of the testis and it is a surgical emergency. Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight antioxidant and potent inhibitor of nuclear factor kappa B (NF-κB) activation. In this study, we aimed to investigate the effects of PDTC to testicular torsion-detorsion (T/D) injury. Forty adult male Sprague-Dawley rats were separated into four groups. A sham operation was performed in group I. In group II, torsion is performed 2 hours by 720 degree extravaginally testis. In group III, 4 h reperfusion of the testis was performed after 2 h of testicular torsion. In group IV, after performing the same surgical procedures as in group III, PDTC (100 mg/kg, intravenous's) was administered before 30 min of detorsion. The testes tissue malondialdehyde (MDA), superoxide dismutase (SOD) catalase (CAT) level was evaluated. Histological evaluations were performed after hematoxylin and eosin staining. Testicular tissue MDA levels were the highest in the T/D groups compared with treatment group. Administration of PDTC prevented a further increase in MDA levels. Significant decrease occurred in CAT and SOD levels in treatment group compared with the control group. The rats in the treatment group had normal testicular architecture. The results suggest that PDTC can be a potential protective agent for preventing the biochemical and histological changes related to oxidative stress in testicular injury caused by testis torsion.

Adalimumab mitigates ovarian ischemia–reperfusion injury in rats by regulating oxidative stress, apoptosis and resolution of inflammation

“…Various physiopathological mechanisms have been put forward to explain the tissue damage that occurs during ovarian torsion and detorsion including ovarian lipid peroxidation, vascular leucocyte margination and apoptosis . The detorsion involves the production of toxic reactive oxygen species (ROS) with the return of blood flow following ischemia . Overproduction of ROS could lead to a significant elevation in lipid peroxidation, thereby generating MDA and destroying the antioxidant defense systems of the human body .…”

Effects of Nitrate Intake on Myocardial Ischemia-Reperfusion Injury in Diabetic Rats

“…Levels of malondialdehyde (MDA), an indirect marker of cellular injury that reflects the extent of systemic lipid peroxidation in the heart, were measured by the method by Oshawa et al 18 In brief, heart samples were collected at baseline and during the post-IR period and then homogenized in phosphate buffered saline (PBS) (1:5, w/v) using a mini homogenizer (MICCRA-D1, Germany). Tissue homogenates were centrifuged (1,000 g, 4 ºC, 10 min) and supernatants were collected for measurement of MDA levels; 2.5 mL of trichloroacetic acid (20%) was added to 0.5 mL of supernatant followed by addition of 1 mL of thiobarbituric acid (0.67%).…”

Effects of sepsis on the metabolism of sphingomyelin and cholesterol in mice with liver dysfunction

“…Contrastingly, the expression of Apo A1 was downregulated compared to the control group. However, when mice were intraperitoneally injected with PDTC, which may attenuate the inflammatory response by inhibiting NF-κB, all of the changes induced by LPS were reversed (19,20). These results indicated that sepsis directly affected the SM and cholesterol content, and the expression of these proteins in the livers of mice.…”

“…To clarify the metabolism of SM and cholesterol in the liver during sepsis in the present study, BALB/c mice were treated with LPS (to induce sepsis), LPS + pyrrolidine dithiocarbamate (PDTC) or PBS. PDTC inhibits the activation of NF-κB specifically by suppressing the release of the inhibitory subunit IκB combining with NF-κB (19,20). SM and cholesterol content, SMS activity and related protein levels were measured.…”