Adalimumab mitigates ovarian ischemia–reperfusion injury in rats by regulating oxidative stress, apoptosis and resolution of inflammation

Abstract

Aim: Ovarian torsion is a rare but an important reason of acute lower abdominal pain in women and associated with serious morbidity and mortality, if not treated promptly. The aim of this study was to evaluate the effects of an antitumor necrosis factor-α antibody on ovarian torsion in a rat model of ischemiareperfusion (I/R) injury. Methods: Forty female Wistar Albino rats were used in the present study. The rats were randomly divided into four groups: group I (sham), group II (I/R), group III (I/R + isotonic saline) and group IV (I/R + adalimumab). The I/R model was induced by torsion of both ovaries. Immunohistochemical staining for interleukin-1β (IL-1β), nuclear factor-κB (NF-κB), and inducible nitric oxide synthase was performed. Tissue and serum oxidative stress markers in conjunction with apoptotic index (AI) with the terminal deoxynucleotidyl transferase dUTP nick end labeling method were also calculated. Results: Tissue total oxidant status, oxidative stress index and nitric oxide values were significantly decreased, and tissue total antioxidant status was found to be increased in group IV. Inflammation, vascular congestion and hemorrhagia were significantly lower in adalimumab-treated group. Serum oxidative stress markers and tissue malondialdehyde levels did not differ in study groups. The AI was significantly increased in groups 2 and 3. Adalimumab treatment significantly decreased the AI. Conclusion: Adalimumab therapy in rats attenuated I/R induced ovarian injury, possibly suppressing inflammation, inhibiting oxidative stress, and altering apoptotic pathways.

Evaluation of the protective effects of gossypin for ischemia/reperfusion injury in ovary tissue

“…In our study, it was observed that NF‐κB expression increased in the ovarian tissue following I/R compared to the healthy group. This result is consistent with previous ovarian I/R studies 13,45 . In addition, in our study, a dose‐dependent decrease in NF‐κB expression was observed in the groups receiving GOS.…”

“…This result is consistent with previous ovarian I/R studies. 13,45 In addition, in our study, a dose-dependent decrease in NF-κB expression was observed in the groups receiving GOS. This result is in agreement with Cinar et al who reported that the application of GOS decreased elevated NF-κB expression after sepsis-induced injury.…”

The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study

“…Cat No: 84029) antibodies. Light microscopic (Olympus CX43) evaluation of immunohistochemically stained sections was Scored as described previously in the literature as 0, no staining; 1, weak but detectable staining; 2, moderate staining; and 3, strong staining [17].…”

The effect of protection of platelet‐rich plasma against experimental ischemia/reperfusion injury in the rat ovary on in vitro fertilization outcomes

“…When published literature was evaluated, the protective effects of many agents against I‐ and I/R‐related damage to the ovarian tissue as a result of ovarian torsion have been demonstrated histopathologically or with serum biochemical markers 8,12,13 . The protective effect of PRP on ovarian torsion has been shown in previous studies in the context of histopathology and serum biochemical markers 14 .…”