Protective Effects of Sweet Orange Peel (Citrus Sinensis L.) The Induction of Micronuclei Induced by Cyclophosphamide in Human Peripheral Lymphocytes

Abstract

Recycling of fruit waste is one of the most important ways of utilizing it in a number of novel products , as well as in medicine, so came this research to verify the protective role of the extracts of sweet orange (Citrus sinensis L.) peels against genotoxicity induced by cyclophosphamide on human peripheral lymphocytes using the micronucleus and chromosomal aberration tests.Conducted overlap between Aqueous and alcohol extracts orange peel with the drug, through three types of transactions (before, after, with treatment). In order to test the effectiveness of the extracts, three concentrations of 5, 10 and 15 µg / ml where tested the prevention or minimization the effect of the drug (80 µg / ml) on human blood lymph . The extracts of the orange peel has antimutagenic potential induced by cyclophosphamide this may prevent the mutagenic effect of various genotoxic or carcinogenic agents, and thus utilization of fruit waste and products for therapeutic purpose. Contribution/ OriginalityThis study is one of very few studies which have investigated of antimutagenic effect of fruit waste such as orange peel against cytotoxic effect of cyclophosphamide , The paper's primary contribution is its finding that these fruit waste can be used to prevent the mutagenic effect of various genotoxic or carcinogenic agents . These findings contribute to the understanding of the health benefits of fruit waste. This study originates new formula to recycling of fruit waste and benefit from it in the therapeutic purpose.

Antimutagenic and antioxidant activity of the essential oils of Citrus sinensis and Citrus latifolia

“…The Citrus genus has previously been studied that reduce DNA damage by alkylating mutagens like methyl-methane-sulfonate (MMS), cyclophosphamide (CP) and ifosfamide. Orange juice prevented and repaired the damage induced by MMS and CP in comet assay and the grapefruit juice reduced the micronucleus formation and sister chromatid exchanges in vivo 20 – 22 . The antimutagenic properties of the EOs of C. sinensis and C. latifolia observed in the present work are in accordance with the aforementioned studies on the antimutagenic effect against alkylating agents.…”