Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells

Hou, Rolis Chien-Wei; Huang, Hsueh‐Meei; Tzen, Jason T. C.; Jeng, Kee-Ching

doi:10.1002/jnr.10749

Cited by 112 publications

(96 citation statements)

References 44 publications

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“…Cells were also pretreated with isochaihulactone ( Figure 1) or 100 µmol/L α-tocopherol (a potent antioxidant) 3 h before the addition of H 2 O 2 [33] . Pretreatment with isochaihulactone (5 μmol/L or 10 μmol/L) significantly (P<0.05) inhibited this decrease (Figure 2A) 27.6% (10 μmol/L). Pretreatment with 100 μmol/L α-tocopherol also significantly attenuated this increase in LDH release.…”

Section: Isochaihulactone Protected Npc12 Cells Against H 2 O 2 -Indumentioning

confidence: 89%

“…Isochaihulactone (also known as K8) is a lignan compound that was identified in acetone extracts of Nan-Chai-Hu and shows antitumor activity against A549 cells in vitro and in vivo [25,26] . Lignan compounds (eg, sesamin, sesamolin) have been reported to act as neuroprotective agents against oxidative injury and excitotoxicity [27][28][29][30] . Lignans can also inhibit lipopolysaccharide-inducible COX-2 expression in macrophages [31] .…”

Section: Introductionmentioning

confidence: 99%

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Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Lin²,

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the effect of isochaihulactone (also known as K8), a lignan compound of Bupleurum scorzonerifolium, on H 2 O 2 -induced cytotoxicity in neuronally differentiated PC12 cells (nPC12). Methods: Viability of neuronal PC12 cells was measured using MTT assay. Protein expression was determined by Western blot. Apoptotic cells was determined using TUNEL assay. D-galactose aging mice were used as a model system to study the anti-oxidant effects of isochaihulactone in vivo. Results: Pretreatment with isochaihulactone (5-10 μmol/L) increased cell viability and decreased membrane damage, generation of reactive oxygen species and degradation of poly (ADP-ribose) polymerase in H 2 O 2 -treated nPC12 cells and also decreased the expression of cyclooxygenase-2, via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. The results suggest that isochaihulactone is a potential antioxidant agent. In a murine aging model, in which chronic systemic exposure to D-galactose (D-gal) causes the acceleration of senescence, administration of isochaihulactone (10 mg·kg -1 ·d -1 , sc) for 7 weeks concomitant with D-gal injection significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, H&E staining to quantify cell death within hippocampus showed that percentage of pyknotic nuclei in the D-gal-treated mice were much higher than in control. Conclusion: The results suggest that isochaihulactone exerts potent anti-aging effects against D-gal in mice possibly via antioxidative mechanisms.

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Section: Isochaihulactone Protected Npc12 Cells Against H 2 O 2 -Indumentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Lin²,

et al. 2010

Acta Pharmacol Sin

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“…In recent years, more and more researchers have investigated the function of natural compounds. Many studies show a number of products provide antihypoxia action, including sesamin, seasmolin, ginkgolid, and an extract of Curcuma longa L. (Zingiberaceae) (Chen et al, 2001;Hou et al, 2003;Koo et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Salidroside on Hypoxia/Reoxygenation Injury by Sodium Hydrosulfite in PC12 Cells

Chen

Lü

Wang

et al. 2007

Pharmaceutical Biology

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Hypoxia=reoxygenation causes cellular injury and death associated with many pathophysiologic conditions, including respiratory disorders, myocardial ischemia, and tumour progression diseases. Neuronal pheochromocytoma (PC12) cells are widely used as a model system for neurologic research and are subject to chemical hypoxia induced with sodium hydrosulfite (Na 2 S 2 O 4 ), a common oxygen-consuming agent. Salidroside, which is the main active component of the famous traditional Chinese herb Rhodiola rosea L. (Crassulaceae), has been proved to possess many bioactivities. In this article, we studied the protective effects of salidroside on hypoxia= reoxygenation injury in PC12 cells induced by Na 2 S 2 O 4 . Cultures of PC12 cells were exposed for 1 h to 10 mM Na 2 S 2 O 4 for hypoxia, followed by reoxygenation for 2 h. The results showed that salidroside was very stable in medium and was not harmful to PC12 cells at the experimental concentrations of 0 $ 200 mg=mL. The cytoprotection by salidroside was dose-dependent, and the cell viability was 41.8 AE 5.7%, 62.4 AE 4.1%, and 92.2 AE 3.7% at 0, 50, and 100 mg=mL of salidroside, respectively. The level of released LDH significantly decreased from 513.5 AE 5.5% (without salidroside) to 258.1 AE 6.3% (with 100 mg=mL salidroside). Flow cytometry was performed to measure apoptotic rate. The results of flow cytometry assay indicated that the apoptotic rate was 17.0 AE 1.2% after hypoxia=reoxygenation injury. When the cells were treated with salidroside 12.5, 50 and 100 mg=mL, the apoptotic rate was 9.5 AE 0.9%, 7.4 AE 0.5%, and 4.5 AE 0.4%, respectively. In addition, our results were confirmed by inspection of cell morphology of PC12 cells. Treatment with salidroside (12.5, 50, 100 mg=mL) significantly prevented the cells from morphologic changes. All the above results showed salidroside could effectively protect PC12 cell against hypoxia=reoxygenation injury.

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“…However recent studies suggested several molecular mechanisms for natural antioxidants-mediated neuroprotection against H 2 O 2 intoxication. In fact, these mechanisms were found to be related with activation of the PI3K/Akt and alpha 2-adrenergic signalling pathways, tyrosine kinase of Trk receptors, inhibition of n-methyl-daspartic acid (NMDA) receptors, and regulation of apoptosis (Hou et al 2003;Shin et al 2003;Jiang et al 2003;Liu et al 2010;Iizuka et al 2010;Zhang et al 2012;Vlasova et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

et al. 2014

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Sesquiterpenes have attracted much interest with respect to their protective effect against oxidative damage that may be the cause of many diseases including several neurodegenerative disorders and cancer. Our previous unpublished work suggested that cyclosativene (CSV), a tetracyclic sesquiterpene, has antioxidant and anticarcinogenic features. However, little is known about the effects of CSV on oxidative stress induced neurotoxicity. We used hydrogen peroxide (H 2 O 2 ) exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of CSV in H 2 O 2 -induced toxicity in newborn rat cerebral cortex cell cultures for the first time. For this aim, MTT and lactate dehydrogenase release assays were carried out to evaluate cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to evaluate oxidative changes. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels, the single cell gel electrophoresis (or Comet assay) was also performed for measuring the resistance of neuronal DNA to H 2 O 2 -induced challenge. Our results showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage (Comet assay) increased in the H 2 O 2 alone treated cultures. But pretreatment of CSV suppressed the cytotoxicity, genotoxicity and oxidative stress which were increased by H 2 O 2 . On the basis of these observations, it is suggested that CSV as a natural product with an antioxidant capacity in mitigating oxidative injuries in the field of neurodegenerative disorders.

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Protective effects of sesamin and sesamolin on hypoxic neuronal and PC12 cells

Cited by 112 publications

References 44 publications

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Protective Effects of Salidroside on Hypoxia/Reoxygenation Injury by Sodium Hydrosulfite in PC12 Cells

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

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