Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

Türkez, Hasan; Toğar, Başak; Stefano, Antonio Di; Taspınar, Numan; Sozio, Piera

doi:10.1007/s10616-013-9685-9

Cited by 29 publications

(11 citation statements)

References 60 publications

(54 reference statements)

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“…Oxidative damage to DNA is quantitated in studied tissues of experimental animals by measuring the increases of 8-O-HdG formations (Neophytou et al 2014). 8-OHdG is also used for risk assessment of cancers and degenerative diseases (Valavanidis et al 2009;Turkez et al 2015). Our study found that the increasing doses of CAR in rat pancreas decreased pancreatitis-induced 8-OHdG levels.…”

Section: Discussionmentioning

confidence: 66%

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

et al. 2015

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Acute pancreatitis (AP) is considered as major problem around the world and the incidence of AP is increasing. Carvacrol (CAR), a monoterpenic phenol, has good antioxidant activity. This in vivo study was designed to evaluate whether CAR provide protection against AP that developed by pancreas injury. The rats were randomised into groups to receive (I) no therapy; (II) 50 lg/kg cerulein at 1 h intervals by four intraperitonally (i.p.) injections; (III) 50, 100 and 200 mg/kg CAR by one i.p. injection; and (IV) cerulein plus CAR after 2 h of cerulein administration. 12 h later, serum samples were obtained to assess pancreatic function, the lipase and amylase values. The oxidative stress markers were evaluated by changes in the amount of lipid peroxides measured as malondialdehyde (MDA) and changes in main tissue antioxidant enzyme levels including SOD, CAT and GSH-PX. Histopathological examination was performed using scoring systems. Additionally, oxidative DNA damage was determined by measuring the increases of 8-hydroxy-deoxyguanosine (8-OH-dG) formations. We found that the increasing doses of CAR decreased AP-induced MDA and 8-OH-dG levels. Moreover, the pancreas antioxidant enzyme activities were higher than that of the rats in the AP group when compared to the AP plus CAR group. In the treatment groups, the lipase and amylase were reduced. Besides, histopathological findings in the pancreatic tissue were alleviated (p \ 0.05). We suggest that CAR could be a safe and potent new drug candidate for treating AP through its antioxidative mechanism of action for the treatment of a wide range of disorders related to pancreas.

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Section: Discussionmentioning

confidence: 66%

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

et al. 2015

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“…In this way, very low levels of side effects were observed against different cancer types (Shen et al 2007, Russo et al 2010. In these studies, plant extracts and components had an important role (Sadi et al 2016, Turkez et al 2015.…”

Section: Introductionmentioning

confidence: 87%

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Emsen

Özdemir

Engin

et al. 2019

An. Acad. Bras. Ciênc.

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Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed glial-neuronal non-cancerous cells from Sprague-Dawley rats were also utilized to measure the effects of treatments on non-cancerous cells. Based on median inhibitory concentration values, the data belonged to non-cancerous cells (2486.71 mg/l) showed distinct towering compared to U87MG (80.93 mg/L) cells. The viability of non-cancerous and U87MG cells exposed to extract is decreased in a dose dependent manner. It was also showed that low concentrations of extract notably increased total antioxidant capacity on non-cancerous cells. In addition, various phenolic compounds in extract were detected through high-performance liquid chromatography. The recent results encourage that extract will be able to have therapeutic potential against glioblastoma.

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“…100 μL of reaction mixture was added to each well and incubated for 30 min at room temperature. Finally, the absorbance of the cultures was measured at 490 nm using a microplate reader [ 68 ].…”

Section: Methodsmentioning

confidence: 99%

Piplartine Analogues and Cytotoxic Evaluation against Glioblastoma

et al. 2018

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Piplartine (1) is an alkamide extracted from plants of the genus Piper which shows several pharmacological properties, including antitumor activity. To improve this activity, a series of analogues based on 1 have been synthesized by esterification and amidation using the 3,4,5-trimethoxycinnamic acid-like starting material. During the study, the moieties 3-(3,4,5-trimethoxyphenyl)acrylate and 3-(3,4,5-trimethoxyphenyl)acrylamide were maintained on esters and amides respectively. Meanwhile, functional changes were exploited, and it was revealed that the presence of two aromatic rings in the side-chain was important to improve the cytotoxic activity against the U87MG cell line, such as the compound (E)-benzhydryl 3-(3,4,5-trimethoxyphenyl)acrylate (10), an ester that exhibited strong cytotoxicity and a similar level of potency to that of paclitaxel, a positive control. Compound 10 had a marked concentration-dependent inhibitory effect on the viability of the U87MG cell line with apoptotic and oxidative processes, showing good potential for altering main molecular pathways to prevent tumor development. Moreover, it has strong bioavailability with non-genotoxic and non-cytotoxic properties on human blood cells. In conclusion, the findings of the present study demonstrated that compound 10 is a promising agent that may find applications combatting diseases associated with oxidative stress and as a prototype for the development of novel drugs used in the treatment of glioblastoma.

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Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

Cited by 29 publications

References 60 publications

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Piplartine Analogues and Cytotoxic Evaluation against Glioblastoma

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