Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Abstract

Aim: To investigate the effect of isochaihulactone (also known as K8), a lignan compound of Bupleurum scorzonerifolium, on H 2 O 2 -induced cytotoxicity in neuronally differentiated PC12 cells (nPC12). Methods: Viability of neuronal PC12 cells was measured using MTT assay. Protein expression was determined by Western blot. Apoptotic cells was determined using TUNEL assay. D-galactose aging mice were used as a model system to study the anti-oxidant effects of isochaihulactone in vivo. Results: Pretreatment with isochaihulactone (5-10 μmol/L) increased cell viability and decreased membrane damage, generation of reactive oxygen species and degradation of poly (ADP-ribose) polymerase in H 2 O 2 -treated nPC12 cells and also decreased the expression of cyclooxygenase-2, via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. The results suggest that isochaihulactone is a potential antioxidant agent. In a murine aging model, in which chronic systemic exposure to D-galactose (D-gal) causes the acceleration of senescence, administration of isochaihulactone (10 mg·kg -1 ·d -1 , sc) for 7 weeks concomitant with D-gal injection significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, H&E staining to quantify cell death within hippocampus showed that percentage of pyknotic nuclei in the D-gal-treated mice were much higher than in control. Conclusion: The results suggest that isochaihulactone exerts potent anti-aging effects against D-gal in mice possibly via antioxidative mechanisms.

Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice

“…Similar changes of normal aging animals have been reported by Yu et al ) 35 ). Liu et al have reported that reproductive aging has been implicated by OS ( 36 ).…”

“…Liu et al have reported that reproductive aging has been implicated by OS ( 36 ). Somatic aging in general, is involved in reproductive aging due to OS ( 35 ). OS causes damage to oocyte and embryos.…”

D-Galactose Induces a Mitochondrial Complex I Deficiency in Mouse Skeletal Muscle: Potential Benefits of Nutrient Combination in Ameliorating Muscle Impairment

“…-galactose (D-gal), a physiological nutrient derived from lactose in milk, 1 is metabolized in animals by D-galactokinase and galactose-1-phosphate uridyltransferase (GALT). Although some researchers have challenged the D-gal-induced aging model, 2,3 some other researchers indicate that D-gal-treated animals showed some hallmarks of aging, such as a shortened lifespan, 4 cognitive dysfunction, 5 presbycusis, 6 increased oxidative stress, 4,7 decreased antioxidant enzyme activity, 8 diminished immune responses, 9 increased advanced glycation endproducts, 10 accumulation of mitochondrial DNA mutations, 11 and mitochondrial dysfunction.…”

Human Serum Albumin-Inspired Glycopeptide-Based Multifunctional Inhibitor of Amyloid-β Toxicity

“…The effect of Glupep on Aβ42–Cu 2+ -induced ROS generation was studied using the DCFDA assay in PC12 cells. 59 DCFDA (2,7-dichlorofluorescein diacetate) is a nonfluorescent compound utilized to quantify ROS strains such as hydroxyl, peroxyl, and other ROS species. DCFDA reacts with the ROS molecules formed from the Aβ42–Cu 2+ -mediated redox cycle and gets oxidized to fluorescent 2,7-dichlorofluorescein (DCF, λ ex = 495 nm and λ em = 529 nm).…”