Protective effects of polydatin against CCl₄-induced injury to primarily cultured rat hepatocytes

Abstract

AIM To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl4. METHODS Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5×10 5 cells/mL). Two mL or 0.2mL was added into 24-well or 96-well plates respectively. Twenty-four hours after cell preculture, PD at concentrations of 10 -7 mol/L-10 -4 mol/L was added into each plate. At the same time injury to hepatocytes was induced by adding 10mmol/L-CCl 4 . Then, 0.1mL or 1mL-culture solution was removed from the 96-well or 24-well plates at 6h, 12h, 2 4h and 48h after CCl 14 intoxication respectively for the determination of GPT, GSH and MDA. At 48h, the survivability of rat hepatocytes was assayed by the MTT colormetric method. RESULTS After CCl4 challenge, the release of GPT and the form ation of MDA in rat hepatocytes markedly increased and maintained at a high level in 48h, whereas PD with different concentrations could markedly inhibit this elevation with 10 -5 mol/L PD having the strongest effects and inhibiting rate was over 50%. PD could also improve the decreased content of GSH caused by CCl 4 in accordance with the doses used. CCl 4 evidently decreased the he patocyte survivability from 91.0%±7.9% to 35.4%±3.8%. On the other hand, PD at 10 -7 mol/L-10

Polydatin Ameliorates DSS-Induced Colitis in Mice through Inhibition of Nuclear Factor-kappaB Activation

“…Polydatin (3,4′,5-trihydroxystibene 3-beta-mono-D-glucoside) (l " Fig. 1 A) has been widely studied because of its vast pharmacological activities, including inhibiting the production of inflammatory mediators [12], inducing production of antioxidants [13], regulating immune function [14], preventing mutations [15], inducing the apoptosis of tumor cells [16], protecting the liver [17] and preventing cardiovascular disease [18]. It has been demonstrated that the biomedical properties of resveratrol (trans-3,5,4′-trihydroxystilbene) are similar to those of polydatin as mentioned above despite their disparate polarities (l " Fig.…”

Ameliorative effect of polydatin on oxidative stress-mediated testicular damage by chronic arsenic exposure in rats

“…PD reduces lipid profile in hyperlipidemic rabbits (Xing et al, 2009) and exerts the neuroprotective effect on cerebral injury induced by ischaemia/reperfusion (Cheng et al, 2006). Also, it protects the primarily cultured rat hepatocytes against CCl 4 -induced injury (Huang et al, 1999) and reduces lipid oxidation (Pan et al, 2007). Nonetheless, there has been no report on the reproductive effect of PD on arsenic-induced toxicity in rats, yet.…”

Protective Effects of Polydatin from Polygonum cuspidatum against Carbon Tetrachloride-Induced Liver Injury in Mice

“…Polydatin exhibits favorable fall hematic fat action, which decreases the serum levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), reduces the content of hepatic TG, and depresses the ratios of LDL-C/high-density lipoprotein cholesterol (HDL-C) and TC/HDL-C in hyperlipidemic hamster [16] and rabbit models [17]. CCl 4 -induced rat hepatocyte injury in vitro can also be evidently alleviated by polydatin via regulation of SOD and MDA levels [18]. Nevertheless, there have been no reports on the protective effects of polydatin against liver injury in vivo, as far as we are aware.…”