Protective Effects of Polydatin from Polygonum cuspidatum against Carbon Tetrachloride-Induced Liver Injury in Mice

Abstract

Polydatin is one of main compounds in Polygonum cuspidatum, a plant with both medicinal and nutritional value. The possible hepatoprotective effects of polydatin on acute liver injury mice induced by carbon tetrachloride (CCl4) and the mechanisms involved were investigated. Intraperitoneal injection of CCl4 (50 µl/kg) resulted in a significant increase in the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and hepatic malondialdehyde (MDA), also a marked enhancement in the expression of hepatic tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and nuclearfactor-kappa B (NF-κB). On the other hand, decreased glutathione (GSH) content and activities of glutathione transferase (GST), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were observed following CCl4 exposure. Nevertheless, all of these phenotypes were evidently reversed by preadministration of polydatin for 5 continuous days. The mRNA and protein expression levels of hepatic growth factor-beta1 (TGF-β1) were enhanced further by polydatin. These results suggest that polydatin protects mice against CCl4-induced liver injury through antioxidant stress and antiinflammatory effects. Polydatin may be an effective hepatoprotective agent and a promising candidate for the treatment of oxidative stress- and inflammation-related diseases.

Polydatin: A review of pharmacology and pharmacokinetics

“…PD (0.05-4 mmol/L) decreases NO and MDA contents, inhibits NOS activity, increases SOD, GSH-Px and GSH activities, and suppresses alanine aminotransferase (ALT) release in pyrogallic acid induced hepatocyte culture medium (Mo et al, 1999). The increases in serum aspartate aminotransferase (AST), ALT and hepatic MDA, TNF-a, interleukin (IL)-1b, cyclooxygenase (COX)-2, iNOS and NF-kB in CCl 4 -induced liver injury mice are significantly reversed, and the content of GSH, activities of GSH transferase, SOD, catalase (CAT), GSH peroxidase and mRNA and protein expression levels of hepatic transforming growth factor-b1 (TGF-b 1 ) are also modulated after PD preadministration for 5 continuous days (Zhang et al, 2012a). PD can alleviate hepatic steatosis and reduce plasma and liver concentrations of TG, total cholesterol (TC) and free fatty acid significantly in HFD-induced chronic liver damage rat.…”

Polydatin Protects against Lipopolysaccharide-Induced Fulminant Hepatic Failure in D-Galactosamine-Sensitized Mice

“…Sera and livers were collected for analysis. The dose of PD is based on the results of preliminary experiments in our previous study, and has recently been used in other mice model (14).…”

Hepatoprotective properties of extensively studied medicinal plant active constituents: Possible common mechanisms

“…Future studies will be necessary to expand the existing, limited therapeutic drugs for the vast majority of liver diseases. Reports of medicinal plants for the treatment of liver disease are numerous; however, due to space limitations, we will focus on only a few (Ansari et al, 2011;Asgarpanah & Kazemivash, 2012;Asuku et al, 2012;Avila et al, 2011;Ghosh et al, 2011;Gutierrez et al, 2013;Haddad et al, 2011;Ho et al, 2012;Lee et al, 2012;Mihailovic et al, 2013;Nagalekshmi et al, 2011;Nithianantham et al, 2011;Paul et al, 2013;Shivananjappa et al, 2013;Singab et al, 2010;Mukherjee et al, 2009;Rana et al, 2011;Subramanian et al, 2012;Torres-Gonalez et al, 2011;Upadhyay et al, 2013;Xavier et al, 2012;Zhang et al, 2012).…”