BackgroundThis study aims to establish a diagnostic scoring scheme for Shanghuo (Heatiness) and to evaluate whether Shanghuo is associated with biochemical parameters of salivary lysozyme (LYZ), salivary secreted immunoglobulin (S-IgA), salivary amylase (AMS), and saliva flow rate (SFR).MethodsWe collected 121 Shanghuo patients at the Affiliated Hospitals of Guangzhou University of Traditional Chinese Medicine in Guangdong Province, 60 cases as a Shanghuo recovered group, and 60 healthy cases as a healthy control group. The diagnostic scoring scheme was established by probability theory and maximum likelihood discriminatory analysis on the basis of epidemiology with the design of self-controlled clinical trial. Subsequently, we used the same methods to collect 120 Shanghuo patients, 60 Shanghuo recovered cases, and 60 healthy cases in both Hunan Province and Henan Province. The levels of LYZ, S-IgA, AMS, and SFR were tested when the patients suffered from Shanghuo or recovered, respectively.ResultsThe diagnostic score table for Shanghuo syndrome was established first. In the retrospective tests, the sensitivity, specificity, accuracy, and positive likelihood ratio of the diagnostic score table were 98.9%, 93.5%, 97.5%, and 14.34%, respectively. In the prospective tests, the corresponding values were 94.9%, 85.7%, 91.7%, and 6.64%, respectively. Shanghuo was classified into three degrees based on the diagnostic scores, common Shanghuo: 63–120; serious Shanghuo: 121–150; very serious Shanghuo: >150. A negative correlation was found between Shanghuo and S-IgA (R = -0.428; P = 0.000). The level of S-IgA was also affected by seasonal and regional factors. No significant correlations were found between Shanghuo and the levels of LYZ, AMS, and SFR.ConclusionsIn this study, Shanghuo could be diagnosed by the combination of the diagnostic score table and S-lgA level.
AIM To investigate the protective effects of polydatin (PD) against injury to primarily cultured rat hepatocytes induced by CCl4. METHODS Rat hepatocytes were separated by methods of liver infusion in vivo and cultured medium (7.5×10 5 cells/mL). Two mL or 0.2mL was added into 24-well or 96-well plates respectively. Twenty-four hours after cell preculture, PD at concentrations of 10 -7 mol/L-10 -4 mol/L was added into each plate. At the same time injury to hepatocytes was induced by adding 10mmol/L-CCl 4 . Then, 0.1mL or 1mL-culture solution was removed from the 96-well or 24-well plates at 6h, 12h, 2 4h and 48h after CCl 14 intoxication respectively for the determination of GPT, GSH and MDA. At 48h, the survivability of rat hepatocytes was assayed by the MTT colormetric method. RESULTS After CCl4 challenge, the release of GPT and the form ation of MDA in rat hepatocytes markedly increased and maintained at a high level in 48h, whereas PD with different concentrations could markedly inhibit this elevation with 10 -5 mol/L PD having the strongest effects and inhibiting rate was over 50%. PD could also improve the decreased content of GSH caused by CCl 4 in accordance with the doses used. CCl 4 evidently decreased the he patocyte survivability from 91.0%±7.9% to 35.4%±3.8%. On the other hand, PD at 10 -7 mol/L-10
In the current design method of the eccentrically braced frame structure, the energy dissipation link and the frame beam are both designed as a whole. It is difficult to accurately assess the degree of damage through this method, and it is also hard to repair or replace the energy dissipation link after strong seismic events. Meanwhile, the overall design approach will increase the project’s overall cost. In order to solve the above mentioned shortcomings, the energy dissipation link is designed as an independent component, which is separated from the frame beam. In this paper, the energy dissipation link is bolted to the web of the frame beam. Both finite element simulation and test study of eight groups of energy dissipation links have been completed to study their mechanical behaviors, and the energy dissipation links have been studied in the aspects of length, cross section, and stiffener spacing. The mechanical behaviors include the energy dissipation behavior, bearing capacity, stiffness, and plastic rotation angle. The results indicate clearly that the hysteretic loop of links in the test and finite element analysis is relatively full. By comparing the experimental and finite element simulation data, it can be found that the general shape and trend of hysteretic loop, skeleton curve, and stiffness degradation curve are basically the same. The experiment data explicitly shows that the energy dissipation link of web-connected displays good ductility and stable energy dissipation ability. In addition, the replaceable links possess good rotational capacity when the minimum rotation angle of each specimen in the test is 0.16 rad. The results of the experiment show that the energy dissipation capacity of the link is mainly related to the section size and the stiffening rib spacing of the link. The energy dissipation ability and deformation ability of the link is poorer as the section size becomes larger; meanwhile, these abilities are reduced with the decrease of the stiffening spacing. The experiment result shows that the damage and excessive inelastic deformations are concentrated in the link to avoid any issues for the rest of the surrounding elements, and the links can be easily and inexpensively replaced after strong seismic events. The results are thought provoking, as they provide a theoretical basis for the further study of the eccentrically braced frame structure with replaceable links of web-connected. In future work, the author aims to carry out his studies through optimized design methodology based on the yielding criterion.
The Guangzhou diagnostic score table could be used as the recommended criteria for the diagnosis of FHS. The classification of FHS was basically in conformity with the clinical situation.
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