Protective effects of fullerenol C60(OH)24 on doxorubicin-induced hepatotoxicity in rats: Pathohistological study

Jacević, Vesna; Djordjevic-Milic, Vukosava; Dragojević-Simić, Viktorija; Radić, Nataša; Govedarica, Biljana; Dobrić, Silva; Srdjenović, Branislava; Injac, Rade; Djordjević, Aleksandar; Vasović, Velibor

doi:10.1016/j.toxlet.2007.05.378

Cited by 10 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, these free radicals also mediate oxidation of other cellular molecules and have an important role in the pathogenesis of drug and radiation-induced liver abnormalities. Increased levels of oxidative stress enzymes (SOD, GSH-Px, GR, and CAT) were observed and confirmed in DOX-induced rats [39]. The disturbance in oxidant-antioxidant systems results in tissue injury, which is demonstrated with lipid peroxidation and protein oxidation in the tissue.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Spin-Labeled 1-Ethyl-1-nitrosourea against Oxidative Stress in Liver Induced by Antitumor Drugs and Radiation

Gadjeva

Grigorov

Nikolova

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

This study was carried out to investigate possible protection effect of 1-ethyl-3-[4-(2,2,6,6-tetramethylpiperidine-1-oxyl)]-1-nitrosourea (SLENU), synthesized in our laboratory, against oxidative liver injuries induced in mice treated by antitumor drugs: doxorubicin (DOX), bleomycin (BLM), or gamma irradiation (R). Specifically, alterations in some biomarkers of oxidative stress, such as lipid peroxidation products measured as malondialdehyde (MDA) levels and activities of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied in liver homogenates isolated from tumor bearing C57 black mice after i.p. treatment with solutions of DOX (60 mg/kg), BLM (60 mg/kg), or after total body gamma-irradiation with a single dose of 5 Gy. The same biomarkers were also measured after i.p. pretreatment of mice with SLENU (100 mg/kg). Statistical significant increased MDA levels and SOD and CAT enzymes activities were found in the liver homogenates of tumor bearing mice after alone treatment with DOX or gamma-irradiation compared to the control mice, while these parameters were insignificantly increased after BLM administration compared to the same controls.

show abstract

Section: Discussionmentioning

confidence: 99%

Protective Effect of Spin-Labeled 1-Ethyl-1-nitrosourea against Oxidative Stress in Liver Induced by Antitumor Drugs and Radiation

Gadjeva

Grigorov

Nikolova

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…These results indicate a hepatoprotective action of orally inoculated FNP which is in compliance with the published data of i.p. application of FNP [18,22,24,25].…”

Section: Discussionmentioning

confidence: 99%

“…Serum was used for analysis of enzymatic activity (ALT and AST) and for analysis of urea blood level. All analyses were done in accordance with previously used protocols [23,24]. Each kidney and liver was removed from the sacrificed animals and placed into an ice-cold solution.…”

Section: Biochemical Analysismentioning

confidence: 99%

Protective effects of orally applied fullerenol nano particles in rats after a single dose of doxorubicin

Ivana

Vukmirović

Branislava

et al. 2011

Hem Ind

Self Cite

View full text Add to dashboard Cite

Polyhydroxylated, water soluble, fullerenol C60(OH)24 nano particles (FNP) in vitro and in vivo models, showed an expressive biological activity. The goal of this work was to investigate the potential protective effects of orally applied FNP on rats after a single dose of doxorubicin (DOX) (8 mg/kg (i.p.)) 6 h after the last application of FNP. After the last drug administration, the rats were sacrificed, and the blood and tissues were taken for the analysis. Biochemical and pathological results obtained in this study indicate that fullerenol (FNP), in H2O:DMSO (80:20, w/w) solution given orally in final doses of 10, 14.4, and 21.2 mg/kg three days successively, has the protective (hepatoprotective and nephroprotective) effect against doxorubicin-induced cytotoxicity via its antioxidant properties

show abstract

“…The π–π stacking effect can be utilized for conjugation of aromatic molecules onto the polyaromatic surface of all carbon nanomaterials. Moreover, fullerenes and their derivatives were studied for their efficient quenching of various free radicals and reactive oxygen species, thus potentially serving as radical scavengers and/or anti‐oxidants in biological systems protecting from the oxidative stress and toxicity caused by cytostatic drugs . The combination of the protective properties of fullerenes and their ability of drug delivery make them very attractive for anticancer therapy .…”

Section: Introductionmentioning

confidence: 99%

Fullerene as a transporter for doxorubicin investigated by analytical methods and in vivo imaging

et al. 2014

View full text Add to dashboard Cite

Carbon nanomaterials, including fullerenes, exhibit not only unique structure and electronic properties but also a significant potential to serve as radical scavengers and/or anti-oxidants. Their conjugation with anticancer drugs such as doxorubicin (DOX) may help to balance severe negative side effects of these cytostatics and also improve the delivery of the drug taking advantage of the enhanced cellular uptake, selectivity to cancer cells, and pH regulated release. In this study, the fullerene (C60) surface was oxidized by concentrated nitric acid, which enabled simple DOX-fullerene conjugation based on π-π stacking and hydrophilic interactions with carboxylic groups. The strength of this noncovalent binding is pH dependent. At a low pH, the amino group of DOX is protonated, however at a higher pH, the amino group is deprotonated, resulting in stronger hydrophobic interactions with the fullerene walls. CE and HPLC were employed for characterization of resulting complexes. The cell toxicity of the conjugates was evaluated using Staphylococcus aureus and finally they were administered into the chicken embryo to assess the applicability for in vivo imaging.

show abstract

Protective effects of fullerenol C60(OH)24 on doxorubicin-induced hepatotoxicity in rats: Pathohistological study

Cited by 10 publications

References 0 publications

Protective Effect of Spin-Labeled 1-Ethyl-1-nitrosourea against Oxidative Stress in Liver Induced by Antitumor Drugs and Radiation

Protective Effect of Spin-Labeled 1-Ethyl-1-nitrosourea against Oxidative Stress in Liver Induced by Antitumor Drugs and Radiation

Protective effects of orally applied fullerenol nano particles in rats after a single dose of doxorubicin

Fullerene as a transporter for doxorubicin investigated by analytical methods and in vivo imaging

Contact Info

Product

Resources

About