Protective effects of orally applied fullerenol nano particles in rats after a single dose of doxorubicin

Abstract

Polyhydroxylated, water soluble, fullerenol C60(OH)24 nano particles (FNP) in vitro and in vivo models, showed an expressive biological activity. The goal of this work was to investigate the potential protective effects of orally applied FNP on rats after a single dose of doxorubicin (DOX) (8 mg/kg (i.p.)) 6 h after the last application of FNP. After the last drug administration, the rats were sacrificed, and the blood and tissues were taken for the analysis. Biochemical and pathological results obtained in this study indicate that fullerenol (FNP), in H2O:DMSO (80:20, w/w) solution given orally in final doses of 10, 14.4, and 21.2 mg/kg three days successively, has the protective (hepatoprotective and nephroprotective) effect against doxorubicin-induced cytotoxicity via its antioxidant properties

Potential Medical Use of Fullerenols After Two Decades of Oncology Research

“…In both cases (with Tween and DMSO), FRL created a solution that was stable over a longer period and could be easily reconstituted by placing it on a vortex mixer for a few minutes. [14][15][16][17][18][19][20][21][22][23][24][25][26][27] Using this approach, FRL was found to be highly soluble (still below the value published by Mohan et al 35 ), creating a dark brown solution that could be used for in-vivo administration. In one of our papers, 19 the differences across powder, saline water solution, and solution with co-solvent in water base were reported at the microscopic level.…”

“…The most significant research investigations related to the potential applicability of FRL as organ-protectors in oncology treatments are listed in Table 2. [11][12][13][14][15]19,20,22,24,[75][76][77][78][79][80][81][82][83][84][85] Our extended research team has been publishing several papers focusing on the applicability of FRLs as antioxidants to protect organs from the side effects of chemotherapy [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] and radiotherapy. 11,77,82 Most of the tests were performed with intraperitoneal injection of FRL, [11][12][13][14][15][16][18][19]…”

“…Our first study on the influence of FRL C 60 (OH) 24 on DOX-induced cardiotoxicity in rats was published in 2006, followed by 16 additional papers related to the idea of protecting (as antioxidants) different organs in vivo after the administration of DOX. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] In this review, we summarized the developments made over the past 2 decades, regarding the use of FRLs for medical purposes, with a focus on their applicability in oncology treatments, both as a co-treatment drug in chemotherapy and radiation owing to the antioxidant properties and as a cytostatic drug for major cancer treatment. The available data were reviewed and presented objectively, with all benefits and limitations related to future medical use, providing guidance and recommendations for future investigations.…”

Bioimpact of Carbon Nanomaterials

“…Recently, Icevic et al [106] investigated the protective effect of fullerenol after oral administration in healthy rats in an animal model. The goal of that work was to investigate the potential protective effects of orally applied fullerenol in vivo after a single dose of doxorubicin (8 mg/kg (i.p.))…”

Fullerene as a transporter for doxorubicin investigated by analytical methods and in vivo imaging

“…The π–π stacking effect can be utilized for conjugation of aromatic molecules onto the polyaromatic surface of all carbon nanomaterials. Moreover, fullerenes and their derivatives were studied for their efficient quenching of various free radicals and reactive oxygen species, thus potentially serving as radical scavengers and/or anti‐oxidants in biological systems protecting from the oxidative stress and toxicity caused by cytostatic drugs . The combination of the protective properties of fullerenes and their ability of drug delivery make them very attractive for anticancer therapy .…”