Protective Effects of Epicatechin Against the Toxic Effects of Streptozotocin on Rat Pancreatic Islets: In Vivo and in Vitro

Kim, Myung Joon; Ryu, Gyeong Ryul; Chung, Jae-Eun; Sim, Sang Soo; Min, Do Sik; Rhie, Duck-Joo; Yoon, Shin Hee; Hahn, Sang June; Jo, Yang-Hyeok

doi:10.1097/00006676-200304000-00014

Cited by 118 publications

(68 citation statements)

References 35 publications

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“…Considering that, in a separate pharmacological study, each extract (leaf, stem, root and fruit) elicited a range of in vitro anti-diabetic activities (Martineau et al, 2006), it is interesting that all the identified classes of phenolic compounds, chlorogenic acid, quercetin derivatives, procyanidins and anthocyanins, have demonstrated anti-diabetic potential in vitro and in vivo (Hemmerle et al, 1997;Rodriguez de Sotillo and Hadley, 2002;Kim et al, 2003;Tsuda et al, 2003;Pinent et al, 2004;Ajay et al, 2005;Coskun et al, 2005;Dias et al, 2005;Jayaprakasam et al, 2006 …”

Section: Figurementioning

confidence: 99%

“…Phenolic compounds were emphasised because of their abundance in closely related Vaccinium species (Witzell et al, 2003) and because many different phenolics have demonstrated in vivo anti-diabetic activities (Kim et al, 2003;Coskun et al, 2005). Using a metabolomics-based approach for preliminary compound identification and reference standards for confirmation, chemical profiles for each organ were determined and quantitative comparisons conducted.…”

Section: Introductionmentioning

confidence: 99%

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A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Harris

Burt

Saleem

et al. 2007

Phytochemical Analysis

112

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A method was developed for the analysis of Vaccinium angustifolium Ait. (Lowbush blueberry), which is a widely used natural health product, particularly for the treatment of diabetic symptoms. While the anthocyanin content of the fruit has been well characterized, the chemistry of the vegetative parts used in supportive therapy for diabetes has been largely ignored. Using a metabolomics-based approach for compound identification with an emphasis on phenolic metabolites, a single HPLC-PAD-APCI/ MS method was developed for the separation and quantitation of the major metabolites found in the 95% ethanol extracts of leaf, stem, root and fruit. The leaf extract contained high concentrations of chlorogenic acid (~100 µg/mg extract) and a variety of quercetin glycosides that were also detected in the fruit and stem extracts. Flavan-3-ol monomers (+)-catechin and (−)-epicatechin were found in all plant parts but their procyanidin dimers were exclusively identified in the stem and root. The accuracy and precision of the presented method were corroborated by low intra-and inter-day variations in quantitative results in all plant part extracts. Further validation of the extraction and analytical protocols focused on identified compounds with reputed anti-diabetic activity, revealing recoveries greater than 80% and detection limits of 0.12-2.73 µg/mL.

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Harris

Burt

Saleem

et al. 2007

Phytochemical Analysis

112

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“…Generation of free radicals has been proposed to be a major mechanism involved in the streptozotocin (STZ) cytotoxicity. STZ is frequently used to induce diabetes mellitus in experimental animals through its toxic effects on pancreatic β-cells (Yamagishi et al, 2001;Stefek et al, 2002;Kalendar et al, 2002;Kim et al, 2003). The cytotoxic action of STZ is associated with the generation of reactive oxygen species causing oxidative damage (Szkudelski, 2001).…”

Section: Introductionmentioning

confidence: 99%

Silymarin, a flavonoid antioxidant, protects streptozotocin-induced lipid peroxidation and β-Cell damage in rat pancreas

Sharma¹,

Anwer²,

Pillai³

et al. 2008

Oriental Pharmacy and Experimental Medicine

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SUMMARYThe present study is aimed at finding the influence of silymarin (a flavonoid) (25 mg/kg & 50 mg/kg) in streptozotocin (STZ)-induced diabetic rats. Type 2 diabetes was induced by single intraperitoneal injection of STZ (100 mg/kg) to 3 days old rat pups. Silymarin was administered for 15 days after the animals were confirmed diabetic (75 days after STZ injection). Blood glucose, glycosylated hemoglobin (HbA levels, which were elevated by STZ, were lowered to physiological levels by the administration of silymarin. The levels of LPO were significantly increased in STZ-induced diabetic rats. Silymarin reduced the LPO levels in both pancreas and liver. GSH contents which were reduced significantly in pancreas and liver of STZ-induced diabetic rats were brought back to near normal levels by silymarin treatment. Multifocal necrotic and degenerative changes of pancreas in STZ-diabetic rats were minimized to near normal morphology by administration of silymarin as evident by histopathological examination. Silymarin showed a dose dependent protective effect on STZ-induced β-cell damage. It could be attributed to the antioxidative and free radicals scavenging properties of the flavonoid. Thus, it may be considered as a natural antioxidant with potential therapeutic application in the treatment of type 2 diabetes.

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“…Outras São vários os mecanismos de ação de plantas hipoglicemiantes, como a estimulação da secreção de insulina das células beta das ilhotas pancreáticas ou a inibição da degradação de insulina [46]; adrenomimetismo, bloqueio do canal de potássio na célula beta pancreática [47]; redução da resistência à insulina [46]; regeneração e reparo das células beta pancreáticas [48]; aumento do número de células nas ilhotas pancreáticas [48]; estimulação da secreção de insulina [47]; estimulação da glicogêneses e glicólise hepática [50]; efeito protetor na destruição de células beta pancreáticas [51]; diminuição da atividade do cortisol [52]; inibição da alfa-amilase [53]; prevenção do estresse oxidativo que está relacionado com a disfunção da célula beta pancreática [54].…”

Section: Figura 2 -Distribuição Percentual De Pessoas Que Conhecem Ouunclassified

Estudo etnobotânico de plantas medicinais utilizadas como hipoglicemiantes por usuários do Programa de Fitoterapia da Universidade Federal do Maranhão, Brasil

Santos

2017

Sci. Plena

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O uso de plantas medicinais no tratamento de enfermidades tem ocorrido há muitos anos, sendo a única terapia de certas comunidades. O Diabetes Mellitus é uma desordem metabólica caracterizada pela hiperglicemia, associada a outros fatores de risco. Este trabalho teve como finalidade realizar um levantamento de plantas medicinais utilizadas como hipoglicemiantes por usuários do Programa de Fitoterapia da Universidade Federal do Maranhão – UFMA, São Luís, Maranhão, Brasil. Foram entrevistadas 100 pessoas, através de questionários semiestruturados com obtenção de dados socioeconômicos e variáveis farmacobotânicas com listagem livre de plantas, aplicados entre os meses de março e junho de 2016. A maioria dos entrevistados era do sexo feminino (62%), com idade entre 30 e 59 anos (62%) e ensino médio completo (48%). Foram mencionadas 28 espécies vegetais com a finalidade hipoglicêmica, sendo a berinjela (Solanun melongena L.) a mais citada (31%). As folhas foram as partes da planta mais utilizadas (45%) e infusão foi a forma de preparo mais indicada pelos entrevistados (43%). O estudo das plantas realizado poderá auxiliar estudos posteriores no desenvolvimento de novos medicamentos para o tratamento de diabetes.

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Protective Effects of Epicatechin Against the Toxic Effects of Streptozotocin on Rat Pancreatic Islets: In Vivo and in Vitro

Abstract: EC appears to be helpful in protecting pancreatic islets against exposure to STZ in both in vivo and in vitro systems.

Cited by 118 publications

References 35 publications

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

A single HPLC‐PAD‐APCI/MS method for the quantitative comparison of phenolic compounds found in leaf, stem, root and fruit extracts of Vaccinium angustifolium

Silymarin, a flavonoid antioxidant, protects streptozotocin-induced lipid peroxidation and β-Cell damage in rat pancreas

Estudo etnobotânico de plantas medicinais utilizadas como hipoglicemiantes por usuários do Programa de Fitoterapia da Universidade Federal do Maranhão, Brasil

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