Protective effects of ellagic acid and ozone on rat ovaries with an ischemia/reperfusion injury

Sayar, Ilyas; Biçer, Şenol; Gürsul, Cebrail; Gürbüzel, Mehmet; Peker, Kemal; Işık, Arda

doi:10.1111/jog.12858

Cited by 23 publications

(21 citation statements)

References 48 publications

(85 reference statements)

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“…38 On the contrary, Sayar at al showed that a significant rise in the SOD enzyme activity was detected in the IR group, whereas a significant reduction in the levels of this marker was observed when antioxidant material was applied to an IR injury. 39 Histopathologically, dilated congested blood vessels, inflammation, and severe hemorrhage in ovarian tissue with the oxidative trauma caused by IR were also observed in this study. Confirming our results, similar histopathological changes in rat ovaries related to IR injury were reported in previous experimental studies.…”

Section: Discussionsupporting

confidence: 74%

Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Yılmaz

Gündoğdu

et al. 2020

J Lab Physicians

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Objective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model. Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group 1), 3-hour ischemia, 3-hour reperfusion (IR) (group 2), and IR + 100 mg/kg lycopene (PO) (group 3), IR + 200 mg/kg of lycopene (group 4). The rats’ superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA), and glutathione (GSH) levels were calculated. Ovarian tissue damage was assessed using a histopathological scoring system. Results Serum parameter levels and histological scores showed that treatment with lycopene may be conservative approach to prevent IR injury after the ovarian detorsion procedure.The improvement with lycopene was higher at 200 mg than at 100 mg. The MPO and MDA values were significantly lower in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the MPO and MDA values were lower in group 4 as compared with group 3.The SOD and GSH values were significantly higher in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the SOD and GSH values were higher in group 4 as compared with group 3.Tissue damage scores were elevated in the IR group compared with the sham group, but the treatment with different lycopene doses after reperfusion improved the histopathological tissue damage scores. Conclusion The results showed that lycopene treatment reduced ovarian IR damage. Antioxidant activity was found to increase in a dose-dependent manner. Lycopene treatment may be conservative approach for ovarian torsion patients after the detorsion procedure to prevent IR damage.

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Section: Discussionsupporting

confidence: 74%

Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Yılmaz

Gündoğdu

et al. 2020

J Lab Physicians

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“…As reported, ozone therapy could decrease the level of some apoptotic genes such as caspase1, caspase3, and caspase9 in diabetic kidney tissue (Guclu et al, 2016). Ozone was also considered as an up‐regulator of Bcl‐2/Bax in ovarian tissue (Aslan et al, 2012; Sayar et al, 2016). Our findings of western blotting and qRT‐PCR indicated that caspase3, caspase8, and caspase9 were upregulated and the ratio of Bcl‐2/Bax was downregulated in SH‐SY5Y cells treated with I/R.…”

Section: Discussionmentioning

confidence: 99%

Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

Cai

Zheng

et al. 2020

Cell Biology International

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Cerebral ischemia/reperfusion (I/R) injuries are common and often cause severe complications. Ozone has been applied for protecting I/R injury in animal models of several organs including cerebra, but the detailed mechanism remains unclear. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase measurement were used to determine the influence of ozone on cell activity and damage of SH‐SY5Y cells. Some redox items such as catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH‐Px), and superoxide dismutase (SOD) were measured by enzyme‐linked immunosorbent assay. The mitochondrial membrane potential (ΔΨm) was determined by JC‐1 assay. Cytochrome‐c (cyt‐c) level in the cytoplasm and mitochondrion was measured by western blotting. Apoptosis was determined by flow cytometry, and some apoptosis‐related molecules were detected by quantitative real‐time polymerase chain reaction and western blotting. Ozone alleviated oxidative damage by increasing GSH‐Px, SOD, CAT, and decreasing MDA. Ozone decreased mitochondrial damage caused by I/R injury and inhibited the release of cyt‐c from mitochondrion to cytoplasm in SH‐SY5Y cells. The cell apoptosis caused by I/R was inhibited by ozone, and ozone could decrease apoptosis by increasing the ratio of Bcl‐2/Bax and inhibiting caspase signaling pathway in SH‐SY5Y cells. Ozone has the ability of maintaining redox homeostasis, decreasing mitochondrion damage, and inhibiting neurocytes apoptosis induced by I/R. Therefore, ozone may be a promising protective strategy against cerebral I/R injury.

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“…Histopatho-logical evaluation showed that pre-treatment with peritoneal ozone prevented intestinal mucosal injury caused by I/R ( 20 ). Sayar et al recently reported that medical ozone therapy exhibits a protective effect on rat ovaries in an I/R injury model by reducing oxidative stress ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats

et al. 2017

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Objective:Testicular torsion (TT) refers to rotation of the testis and twisting of the spermatic cord. TT results in ischemia-reperfusion (I/R) injury involving increased oxidative stress, inflammation and apoptosis, and can even lead to infertility. The aim of this study was to investigate the effect of ozone therapy on testicular damage due to I/R injury in an experimental torsion model.Materials and Methods:24 male Sprague-Dawley rats were divided into 3 groups; shamoperated, torsion/detorsion (T/D), and T/D+ozone. Ozone (1mg/kg) was injected intraperitoneally 120 minutes before detorsion and for the following 24h. Blood and tissue samples were collected at the end of 24h. Johnsen score, ischemia modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were determined.Results:Levels of IMA, TOS, OSI, and histopathological scores increased in the serum/tissue of the rats in the experimental T/D group. Serum IMA, TOS, and OSI levels and tissue histopathological scores were lower in the rats treated with ozone compared with the T/D group.Conclusion:Our study results suggest that ozone therapy may exhibit beneficial effects on both biochemical and histopathological findings. Clinical trials are now necessary to confirm this.

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Protective effects of ellagic acid and ozone on rat ovaries with an ischemia/reperfusion injury

Abstract: These biochemical and histopathological findings suggest that EA and O are effective against ovarian I/R injury.

Cited by 23 publications

References 48 publications

Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion‐mediated apoptosis pathway in SH‐SY5Y cells

Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats

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