Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation

Abstract

Objective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model. Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group 1), 3-hour ischemia, 3-hour reperfusion (IR) (group 2), and IR + 100 mg/kg lycopene (PO) (group 3), IR + 200 mg/kg of lycopene (group 4). The rats’ superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA), and glutathione (GSH) levels were calculated. Ovarian tissue damage was assessed using a histopathological scoring system. Results Serum parameter levels and histological scores showed that treatment with lycopene may be conservative approach to prevent IR injury after the ovarian detorsion procedure.The improvement with lycopene was higher at 200 mg than at 100 mg. The MPO and MDA values were significantly lower in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the MPO and MDA values were lower in group 4 as compared with group 3.The SOD and GSH values were significantly higher in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the SOD and GSH values were higher in group 4 as compared with group 3.Tissue damage scores were elevated in the IR group compared with the sham group, but the treatment with different lycopene doses after reperfusion improved the histopathological tissue damage scores. Conclusion The results showed that lycopene treatment reduced ovarian IR damage. Antioxidant activity was found to increase in a dose-dependent manner. Lycopene treatment may be conservative approach for ovarian torsion patients after the detorsion procedure to prevent IR damage.

Neuroprotective effect of roflumilast under cerebral ischaemia/reperfusion injury in juvenile rats through NLRP‐mediated inflammatory response inhibition

“…With the occurrence of ischemia, when physiopathological conditions arise as a result of the inability to provide sustenance to the brain tissue, oxidative stress develops as a result of the restoration of blood flow to ischemic areas through physiological and iatrogenic methods 11 . The increased amount of oxygen causes an excessive amount of free oxygen radicals in tissues adapted to hypoxia, leading to additional reperfusion injuries at the site of ischemic damage 12–14 . Therefore, new approaches that will eliminate or reduce ischemia reperfusion injury in all ischemic conditions are still being investigated 12–16 .…”

“…The increased amount of oxygen causes an excessive amount of free oxygen radicals in tissues adapted to hypoxia, leading to additional reperfusion injuries at the site of ischemic damage 12–14 . Therefore, new approaches that will eliminate or reduce ischemia reperfusion injury in all ischemic conditions are still being investigated 12–16 . Notably, phosphodiesterase (PDE) inhibitors have also been discussed in this context 16 …”

Leptin alleviated ovarian ischemia-reperfusion injury in rats via modulation of Sirt-1/Nrf2 and TLR4/NF-kB/caspase-3 signaling pathways

“…One portion was placed in a 10% formalin for histopathological evaluation, while the other one was preserved at -80 °C for chemical analysis. Then, 0.1 g of the tissue was homogenized in 900 µL of ice-cold phosphatebuffered saline at pH 7.4 (10% w/v) and centrifuged at 4,000 rpm for 15 min at 4 °C (Yilmaz et al 2020). The supernatants were stored at -80 °C for colorimetric assay of malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and nitric oxide (NO) levels (Biodiagnostic, Egypt).…”

“…Ovarian specimens were fixed in 10% formalin solution for 72 h. Paraffinembedded samples (5 mm) were cut and stained with hematoxylin and eosin (H&E). All the sections (multiple sections/rat) were examined under an Olympus (Japan) light microscope (Yilmaz et al 2020). According to the histopathological evaluation system, 5 microscopic fields were examined to assess the degree of congestion, hemorrhage, edema, inflammatory cells infiltration, and follicular degeneration.…”

Effects of Lycopene Attenuating Injuries in Ischemia and Reperfusion

“…Besides, lycopene has also been shown to improve ovarian I/R injury. One study [175] showed that at 2.5 h after ischemia (3 h), lycopene treatment (100 and 200 mg/kg, i.g.) could effectively reduce tissue congestion, hemorrhage, and polymorphonuclear leukocyte infiltration via increasing GSH and CAT and lowering MDA and MPO.…”