Protective effects of dietary grape against atopic dermatitis-like skin lesions in NC/NgaTndCrlj mice

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease with significant health/economic burdens. Existing therapies are not fully effective, necessitating development of new approaches for AD management. Here, we report that dietary grape powder (GP) mitigates AD-like symptoms in 2,4-dinitrofluorobenzene (DNFB)-induced AD in NC/NgaTndCrlj mice. Using prevention and intervention protocols, we tested the efficacy of 3% and 5% GP-fortified diet in a 13-weeks study. We found that GP feeding markedly inhibited development and progression of AD-like skin lesions, and caused reduction in i) epidermal thickness, mast cell infiltration, ulceration, excoriation and acanthosis in dorsal skin, ii) spleen weight, extramedullary hematopoiesis and lymph nodes sizes, and iii) ear weight and IgE levels. We also found significant modulations in 15 AD-associated serum cytokines/chemokines. Next, using quantitative global proteomics, we identified 714 proteins. Of these, 68 (normal control) and 21 (5% GP-prevention) were significantly modulated (≥2-fold) vs AD control (DNFB-treated) group, with many GP-modulated proteins reverting to normal levels. Ingenuity pathway analysis of GP-modulated proteins followed by validation using ProteinSimple identified changes in acute phase response signaling (FGA, FGB, FGG, HP, HPX, LRG1). Overall, GP supplementation inhibited DNFB-induced AD in NC/NgaTndCrlj mice in both prevention and intervention trials, and should be explored further.

Antioxidant Activity of Quercetin-Containing Liposomes-in-Gel and Its Effect on Prevention and Treatment of Cutaneous Eczema

“…The mice were fasted for 12 h after the last treatment, and after execution on day 17, the dorsal skin, liver, and spleen were collected for subsequent experiments. The spleen index was calculated as the ratio of spleen to body weight [ 50 , 51 ].…”

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

“…Singh і співавт. при проведенні гістологічного дослідження селезінки мишей з АД виявили типову архітектоніку тканини червоної та білої пульп [15]. Більше того, збільшення розміру селезінки відбулося на фоні збереження нормальної її мікроархітектури, що, швидше за все, є фізіологічною реакцією на потребу в клітинах крові, які беруть участь в реалізації імунних реакцій [15].…”

“…Singh і співавт. при проведенні гістологічних досліджень селезінки мишей з АД виявили на фоні збереження типової її тканинної архітектоніки деяке збільшення розмірів за рахунок збільшення гемопоетичної зони червоної пульпи [15].…”