Protective effects of Chinese herbs on D-galactose-induced
oxidative damage

Jh, Liu; Sc, Ho; Th, Lai; Th, Liu; Py, Chi; Ry, Wu

doi:10.1358/mf.2003.25.6.769650

Cited by 21 publications

(21 citation statements)

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“…It has been prescribed to treat fever due to common cold, febrile disease, dysentery, carbuncles, and virulent swellings, in Chinese medicine. Many previous reports have shown that LF is an effective antioxidant [2,3] . Chlorogenic acid, one of the major components in LF, has been widely adopted to control the quality of LF, owing to its high content and antibiotic property.…”

Section: Introductionmentioning

confidence: 97%

“…Therapy that merely suppresses gastric acid secretion does not improve the function of the lower esophageal sphincter and oxidative stress is an added important factor in the pathogenesis of reflux esophagitis (RE) in rats [8] . LF has oxidant and antiinflammatory activities [1][2][3][4][8][9][10] ; therefore, in the present study, we determined if an extract of LF could be useful for treating RE in rats, by detecting mucosal damages in an RE rat model.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity

Ku¹,

Seo²,

Park³

et al. 2009

WJG

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AIM:To observe the effects of traditional antiinflammatory medicine Lonicerae Flos (LF) on rat reflux esophagitis (RE) induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, α-tocopherol. M E T H O D S: R a t s w e r e p r e t r e a t e d w i t h t h r e e different dosages of LF (500, 250 and 125 mg/kg) orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), myeloperoxidase and glutathione (GSH) levels, and collagen contents (marker of flexibility) were observed on the esophageal and fundic histopathology. The results were compared with an α-tocopherol (once orally, 1 h before operation, 30 mg/kg) treated group in which the effects on RE were already confirmed. RESULTS:Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects α-tocopherol. CONCLUSION:The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity

Ku¹,

Seo²,

Park³

et al. 2009

WJG

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“…Nan-Chai-Hu (Chai Hu of the South), the root of Bupleurum scorzonerifolium, is an important Chinese herb [24] . Isochaihulactone (also known as K8) is a lignan compound that was identified in acetone extracts of Nan-Chai-Hu and shows antitumor activity against A549 cells in vitro and in vivo [25,26] .…”

Section: Introductionmentioning

confidence: 99%

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Lin²,

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the effect of isochaihulactone (also known as K8), a lignan compound of Bupleurum scorzonerifolium, on H 2 O 2 -induced cytotoxicity in neuronally differentiated PC12 cells (nPC12). Methods: Viability of neuronal PC12 cells was measured using MTT assay. Protein expression was determined by Western blot. Apoptotic cells was determined using TUNEL assay. D-galactose aging mice were used as a model system to study the anti-oxidant effects of isochaihulactone in vivo. Results: Pretreatment with isochaihulactone (5-10 μmol/L) increased cell viability and decreased membrane damage, generation of reactive oxygen species and degradation of poly (ADP-ribose) polymerase in H 2 O 2 -treated nPC12 cells and also decreased the expression of cyclooxygenase-2, via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. The results suggest that isochaihulactone is a potential antioxidant agent. In a murine aging model, in which chronic systemic exposure to D-galactose (D-gal) causes the acceleration of senescence, administration of isochaihulactone (10 mg·kg -1 ·d -1 , sc) for 7 weeks concomitant with D-gal injection significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, H&E staining to quantify cell death within hippocampus showed that percentage of pyknotic nuclei in the D-gal-treated mice were much higher than in control. Conclusion: The results suggest that isochaihulactone exerts potent anti-aging effects against D-gal in mice possibly via antioxidative mechanisms.

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“…Chronic administration of D-galactose (DG) to BL/6J mice has been shown to induce changes which resemble accelerated ageing, such as formation of advanced glycation end products (2,3) , neurological impairment (4,5) , decreased serum antioxidative enzyme activities (4,5) and inflammation in the liver (3,6) . Several antioxidants (3,7,8) and Chinese herbs (9) have been shown to attenuate the ageing damage in C57BL/6J mice treated with DG.Fructo-oligosaccharide (FO), a well-defined prebiotic (10) , has been incorporated into drinks and desserts to improve bowel function in elderly persons (11) . Recent clinical studies have initially shown that the consumption of a prebiotic mix (12) and a FO supplement (13) beneficially reduces the blood indices of peroxidation status.…”

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confidence: 99%

Antioxidative and hepatoprotective effects of fructo-oligosaccharide ind-galactose-treated Balb/cJ mice

et al. 2010

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Chronic subcutaneous (s.c.) administration of D-galactose (DG) to BL/6J mice has been shown to induce oxidative stress and is considered a model to mimic accelerated ageing. Fructo-oligosaccharide (FO) is a well-defined prebiotic and its fermentation by lactic acid bacteria has been shown to exert antioxidative capacity. The present study was aimed to determine whether FO attenuated DG-induced oxidative stress and hepatopathy in Balb/cJ mice. Mice (12 weeks of age, n 40) were divided into control (s.c. saline), DG (s.c. 1·2 g/kg body weight), DG þ FO (5 %, w/w) and DG þ vitamin E (0·2 %, w/w) groups and were killed after 52 d of treatment. Results indicated that DG significantly decreased the hepatic superoxide dismutase and glutathione peroxidase activities. These alterations were ameliorated both by FO and vitamin E. DG increased the hepatic TAG content approximately by 7·2 % compared with the vehicle control, which was in agreement with the histological alteration. FO, similar to vitamin E, almost normalised the hepatic TAG content and ameliorated the histological characteristics of fatty liver. Similarly, the increased plasma alanine aminotransferase activity induced by DG was normalised by FO and vitamin E, respectively. Faecal bifidobacteria counts were greater in the DG þ FO and DG þ vitamin E groups compared with the DG group, respectively. In conclusion, the present study indicated that FO diminished the altered hepatic antioxidative enzyme activities and morphology caused by chronic DG administration in Balb/cJ mice, partially associated with its prebiotic role in the colon.Key words: D-Galactose: Fructo-oligosaccharide: Antioxidative capacity: Liver: Ageing One of the well-recognised ageing theories indicates that generation of oxidative stress leads to cell and tissue damage and ultimately results in ageing and cell death (1) . Chronic administration of D-galactose (DG) to BL/6J mice has been shown to induce changes which resemble accelerated ageing, such as formation of advanced glycation end products (2,3) , neurological impairment (4,5) , decreased serum antioxidative enzyme activities (4,5) and inflammation in the liver (3,6) . Several antioxidants (3,7,8) and Chinese herbs (9) have been shown to attenuate the ageing damage in C57BL/6J mice treated with DG.Fructo-oligosaccharide (FO), a well-defined prebiotic (10) , has been incorporated into drinks and desserts to improve bowel function in elderly persons (11) . Recent clinical studies have initially shown that the consumption of a prebiotic mix (12) and a FO supplement (13) beneficially reduces the blood indices of peroxidation status. However, the effect of FO in DG-induced hepatic oxidative damage has never been demonstrated.The main goal of the present study was to assess the anti-ageing and hepatoprotective effects of FO in DGadministered Balb/cJ mice, by the determination of antioxidative enzyme activities and the morphology of the liver and the biochemical indices of liver function. Materials and methods Animals and dietsMale B...

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Protective effects of Chinese herbs on D-galactose-induced oxidative damage

Cited by 21 publications

References 0 publications

Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity

Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity

Isochaihulactone protects PC12 cell against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-galactose aging mouse model

Antioxidative and hepatoprotective effects of fructo-oligosaccharide ind-galactose-treated Balb/cJ mice

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