Protective Effects of Anethum graveolens Seed’s Oil Nanoemulsion Against Cadmium-Induced Oxidative Stress in Mice

Shafaei, Negin; Barkhordar, Seyed Mohammad Adel; Rahmani, Fatemeh; Nabi, S.U.; Idliki, Reza Baratpour; Alimirzaei, Masoumeh; Karimi, Ehsan; Oskoueian, Ehsan

doi:10.1007/s12011-020-02093-z

Cited by 40 publications

(28 citation statements)

References 34 publications

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“…Anti-inflammatory and antioxidative NEs have been developed as protective and therapeutic formulations for wound healing, and treating neurological, and dermatological diseases [ [166] , [167] , [168] , [169] ]. Anti-inflammatory effects of P-selectin targeted NEs loaded with dexamethasone were studied in animal models.…”

Section: Insights Into Nanoemulsion Drug Delivery Mechanismsmentioning

confidence: 99%

Nanoemulsions: Formulation, characterization, biological fate, and potential role against COVID-19 and other viral outbreaks

Tayeb

Felimban

Almaghrabi

et al. 2021

Colloid and Interface Science Communications

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Section: Insights Into Nanoemulsion Drug Delivery Mechanismsmentioning

confidence: 99%

Nanoemulsions: Formulation, characterization, biological fate, and potential role against COVID-19 and other viral outbreaks

Tayeb

Felimban

Almaghrabi

et al. 2021

Colloid and Interface Science Communications

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“…The main liver enzymes in the serum including alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were determined using blood auto-analyzer (Hitachi 902, Japan). The lipid peroxidation in the liver tissue was determined as described earlier by Shafaei et al [33]. Brie y, the liver tissue was homogenized and 200 µl of lysate were mixed by distilled water (300 µl), BHT (35 µl), sodium dodecyl sulfate (165 µl) and thiobarbituric acid (2 ml) respectively.…”

Section: Liver Enzymes and Lipid Peroxidation Assaymentioning

confidence: 99%

“…Then, they were xed in buffered formalin (10% formalin in 0.1M sodium phosphate buffer, pH7). Finally, they were para nized, sliced, and stained according to the hematoxylin/eosin protocol [33]. The histopathology slides were observed under a light microscope using a magni cation of 20X.…”

Section: Histopathology and Morphometric Analysesmentioning

confidence: 99%

Nanoliposome-encapsulated Phenolic Rich Fraction From Alcea Rosea as a Dietary Phytobiotic in Mice Challenged by Escherichia Coli

Hassirian

Karimi

Oskoueian

2021

Preprint

Self Cite

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Background: This research was performed to evaluate the antibacterial and health-promoting potentials of the nanoliposome-encapsulated phenolic rich fraction (PRF) from Alcea rosea leaves as a dietary phytobiotic in mice challenged by enteropathogenic Escherichia coli (E. coil; O157: H7). Results: The overall results demonstrated that the nanoliposome-loaded PRF contained gallic acid, salicylic acid, pyrogallol, cinnamic acid, catechin, naringin, ferulic acid. The E. coil challenge in mice impaired the weight gain, food intake, liver enzymes, lipid peroxidation, morphometric characteristics of the ileum, up-regulated the inflammatory genes (COX2, iNOS), down-regulated the antioxidant-related genes (SOD and GPx) and increased the population of E. coil in the ileum. The dietary inclusion of nonencapsulated PRF and nanoliposome-encapsulated PRF at the concentration of 10 mg TPC/kg BW/day improved these parameters however the nanoliposome-encapsulated PRF appeared to be more effective as compared to nonencapsulated PRF in improving the health parameters in mice. Conclusion: Consequently, the nanoliposome-encapsulated PRF could play a critical role as a promising phytobiotic against E. coil infection in mice.

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“…The main liver enzymes in the serum including alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were determined using blood auto-analyzer (Hitachi 902, Japan). The lipid peroxidation in the liver tissue was determined as described earlier by Shafaei et al (Shafaei et al 2020). Brie y, the liver tissue was homogenized and 200 µl of lysate were mixed by distilled water (300 µl), BHT (35 µl), sodium dodecyl sulfate (165 µl) and thiobarbituric acid (2 ml) respectively.…”

Section: Liver Enzymes and Lipid Peroxidation Assaymentioning

confidence: 99%

“…Then, they were xed in buffered formalin (10% formalin in 0.1M sodium phosphate buffer, pH7). Finally, they were para nized, sliced, and stained according to the hematoxylin/eosin protocol (Shafaei et al 2020). The histopathology slides were observed under a light microscope using a magni cation of 20X.…”

Section: Histopathology and Morphometric Analysesmentioning

confidence: 99%

Nanoliposome-Encapsulated Phenolic Rich Fraction From Alcea Rosea as a Dietary Phytobiotic in Mice Challenged by Escherichia Coli

Hassirian¹,

Karimi²,

Oskoueian³

2021

Preprint

Self Cite

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Purpose This research was performed to evaluate the antibacterial and health-promoting potentials of the nanoliposome-encapsulated phenolic rich fraction (PRF) from Alcea rosea leaves as a dietary phytobiotic in mice challenged by enteropathogenic Escherichia coli (E. coil; O157: H7). Method: The PEF was encapsulated in nanoliposomes (PEF-NLs) and the phenolic profiling of PEF-NLs was confirmed by HPLC. Then 40 white male balb/c mice at four treatment groups were provided and antibacterial potential of PEF-NLs were assessed by measuring the mice blood parameters and liver lipid peroxidation in mice infected by E. coli. Finally, the expression of cyclooxygenase 2 (COX2), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were determined in miceʼs ileum tissues. Meanwhile, relative fold changes in the ileum population of E. coli was analyzed using Real time PCR. Results The overall results demonstrated that the nanoliposome-loaded PRF contained gallic acid, salicylic acid, pyrogallol, cinnamic acid, catechin, naringin, ferulic acid. The E. coil challenge in mice impaired the weight gain, food intake, liver enzymes, lipid peroxidation, morphometric characteristics of the ileum, up-regulated the inflammatory genes (COX2, iNOS), down-regulated the antioxidant-related genes (SOD and GPx) and increased the population of E. coil in the ileum. The dietary inclusion of nonencapsulated PRF and nanoliposome-encapsulated PRF at the concentration of 10 mg TPC/kg BW/day improved these parameters however the nanoliposome-encapsulated PRF appeared to be more effective as compared to nonencapsulated PRF in improving the health parameters in mice. Conclusion Consequently, the nanoliposome-encapsulated PRF could play a critical role as a promising phytobiotic against E. coil infection in mice.

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Protective Effects of Anethum graveolens Seed’s Oil Nanoemulsion Against Cadmium-Induced Oxidative Stress in Mice

Cited by 40 publications

References 34 publications

Nanoemulsions: Formulation, characterization, biological fate, and potential role against COVID-19 and other viral outbreaks

Nanoemulsions: Formulation, characterization, biological fate, and potential role against COVID-19 and other viral outbreaks

Nanoliposome-encapsulated Phenolic Rich Fraction From Alcea Rosea as a Dietary Phytobiotic in Mice Challenged by Escherichia Coli

Nanoliposome-Encapsulated Phenolic Rich Fraction From Alcea Rosea as a Dietary Phytobiotic in Mice Challenged by Escherichia Coli

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