Protective effect of two extracts of Cydonia oblonga miller (Quince) fruits on gastric ulcer induced by indomethacin in rats

Abstract

Background:In various studies, Cydonia oblonga Miller (quince) has been reported to have many properties such as antioxidant and anti-ulcerative effects. This study has aimed to investigate the protective effects of quince aqueous extract (QAE) and quince hydroalcoholic extract (QHE) on gastric ulcer caused by indomethacin and the relevant macroscopic, histopathology, and biochemical factors in rats.Methods:Ten groups of male Wistar rats, six in each, were used in this study. These groups included: normal (distilled water), control (distilled water + indomethacin), reference (ranitidine or sucralfate + indomethacin), and test groups (QAE or QHE + indomethacin) treated with three increasing doses (200, 500, and 800 mg/kg). Extracts and drugs were given orally to rats 1 h before injecting the indomethacin (25 mg/kg, intraperitoneally). Six hours later, the abdomen of rats was exposed, its pylorus was legated, gastric acid content was extracted, and its pH and the amount of pepsin secreted were measured by Anson method. Then, histopathology indices, ulcer area, ulcer index, and myeloperoxidase (MPO) activity were measured in gastric mucus.Results:Both extracts of quince were effective to reduce the acidity of stomach and pepsin activity. Compared to control group, the average of enzyme activity of MPO was significantly declined in all treated groups. Control group had the highest level of gastric ulcer indices including severity, area, and index while the evaluated parameters had decreased in all extract treated groups although it seems that QAE was somewhat more effective.Conclusions:Protective effect of QAE and QHE on gastric ulcer was done by undermining offensive factors including decreasing the secretion of gastric acid and pepsin activity and by strengthening the protective factors of gastric mucus including antioxidant capacity.

Evaluation of the effect of hydroalcoholic and flavonoid-enriched extracts of Dracocephalum kotschyi on indomethacin-induced gastric ulcer in rats

“…Standard pepsin was used to draw the standard curve for pepsin activity while, 10 min later, 5 mL of trichloroacetic acid was added to terminate the reaction. After filtration, optical absorbance of amino acids produced by the reaction of pepsin with hemoglobin was measured at the wavelength of 280 nm, and pepsin activity was determined in terms of mg after 15 min ( 30 ).…”

“…The stomach was then cut into two longitudinal sections for further examination. One section was placed into formalin solution (10%) and sent for pathological examination to assess inflammation severity, hemorrhagic spot, sub-mucosal edema, and superficial mucosal ulcers ( 30 ). The other section was used for the measurement of myeloperoxidase (MPO) activity and malondialdehyde (MDA) value.…”

“…MPO activity was assessed by the method previously set up in our laboratory ( 31 ). MDA activity was analyzed by lipid peroxidation (MDA) assay kit (Navand-Salamat Lab Kit, Iran) according to package insert protocol was carried out previously ( 29 30 ).…”

Formulation of Pomegranate Seed Oil: A Promising Approach of Improving Stability and Health‐Promoting Properties

“…[65] This could possibly be attributed to diverse compositions, which are responsible for the synergistic effects of natural products with PSO not an exception. Recently, some authors have suggested the possible mechanisms of the active compounds in PSO [61,62,[66][67][68] based on the physiological effect studied ( Figure 2). Lately, PSO was shown to promote peak bone mass via increased insulin-like growth factor type 1 (IGF-1) expression in liver as well as signaling in bone.…”

Estudio Comparativo Del Efecto Espasmolítico Del Membrillo (Cydonia Vulgaris) en El Tránsito Intestinal

“…Dentro de sus usos medicinales, encontramos que tradicionalmente diferentes partes de las plantas tales como raíces, frutas, hojas y semillas se utilizan para el tratamiento de varias enfermedades, entre ellas: diarrea, tos, disentería, dolor de garganta, estreñimiento y bronquitis 9,10 . Además, de forma industrial, su fruto es usado como protector mucoso en el Síndrome de intestino irritable y úlcera péptica 11,12 .…”