Evaluation of the effect of hydroalcoholic and flavonoid-enriched extracts of Dracocephalum kotschyi on indomethacin-induced gastric ulcer in rats

Minaiyan, Mohsen; Sadraei, Hassan; Yousefi, Iman; Sajjadi, Sayed-Ebrahim; Talebi, Ardeshir

doi:10.4103/1735-5362.310521

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…15 Because of its inhibitory effect on prostaglandin synthesis, it can harm stomach tissue by increasing gastric acid and pepsin activity and lipid peroxidation and oxidative stress by generating free radicals like (MDA, and MPO). 16 In this study the sites where diclofenac sodium-induced gastric mucosal lesions were the glandular portion of the rat's stomach. The macroscopic appearance of these mucosal lesions is in accordance with the finding, in which they expressed a very similar gastric mucosal damage with close features for this current study.…”

Section: Discussionmentioning

confidence: 91%

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Abdelfatah¹,

Al-Qadh²

2023

IJDDT

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Introduction: Non-steroidal anti-infl ammatory drugs (NSAIDs), are widely prescribed in clinical practice because of their ability to reduce pain, fever, and infl ammation. However, NSAID-induced gastric mucosal damage is the major side eff ect of these medications. This study aims to reduce gastric mucosal lesions caused by NSAIDs by using pirfenidone by investigating their eff ect on histological, gastric gross mucosal damage. Method: 30 healthy male albino rats were divided into 3 groups each of ten (N=10). A single oral dose of diclofenac sodium (150 mg/kg body weight) was used to induce ulceration for all groups except fi rst. The vehicle (tween 80+NS) was given to the fi rst group, while diclofenac sodium was given to the second group and to all pre-treated groups. The third group were pre-treated with pirfenidone (300 mg/kg). At the end of the experiment, histological examination, and antioxidant and antiinfl ammatory parameters by immunohistochemistry method were evaluated. Results: Diclofenac sodium at a dose (150 mg/kg) produces a signifi cant increment (p > 0.01) in gastric damage score, the expression of tumor necrosis factor-alpha (TNF-α), myeloperoxidase, malonaldehyde, and the ulcer formation percent, compared to the healthy rat’s group. Pirfenidone at a dose of (300 mg/kg) pretreatment in diclofenac induced-ulcer in rats produces a signifi cant reduction (p > 0.01) in gastric damage score, the expression of TNF-alpha, myeloperoxidase, the expression of TNF-alpha, myeloperoxidase, malonaldehyde, and in the ulcer formation percent, yet, less eff ectively than omeprazole and pirfenidone. Conclusion: Diclofenac can be reduced or prevented by the pretreatment of pirfenidone. Pirfenidone showed similar results to the standard treatment (Omeprazole), the protective eff ect of pirfenidone was mainly through their antioxidant and anti-infl ammatory activity by reducing oxidation markers like MPO and MDA, and also reducing infl ammatory cytokines like TNF-alpha

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Section: Discussionmentioning

confidence: 91%

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Abdelfatah¹,

Al-Qadh²

2023

IJDDT

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“…Zaringiah is an Iranian herbal plant that is commercially cultivated for herbal medicinal utilization. Pharmacological investigations have revealed that in comparison with the other recognized herbal medicines possessing antispasmodic activity, D. kotschyi extract has a higher potency, efficacy, and relative selectivity (8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Therefore, it was selected as suitable candidate or further pharmacological evaluations.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, the hydroalcoholic extract of D. kotschyi has been shown to inhibit ileum contractions both in vitro and in vivo situations (8)(9)(10)(11). In addition, it has anti-inflammatory effects on the stomach ulcer and induced colitis in animal models (15,16). Furthermore, D. kotschyi extract inhibits bronchial smooth muscle contractions and has shown both anti-inflammatory and anti-fibrotic properties in bleomycin induced bronchitis (12,14).…”

Section: Introductionmentioning

confidence: 99%

Bioassay standardization of drug dosage form prepared from hydroalcoholic extract of Dracocephalum kotschyi

Sadraei

Rasouli-Amirabadi

Yegdaneh

et al. 2022

J Herbmed Pharmacol

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Introduction: Zaringiah (Dracocephalum kotschyi) is a famous Iranian herbal plant with anti-inflammatory and spasmolytic activities. There is no standard drug dosage form for the D. kotschyi extract on the market. The objective of this project was to design a suitable oral dosage form for the hydroalcoholic extract of D. kotschyi. Methods: Standard granules were prepared using the moist granulation technique. Physical properties of the granules were determined before filling the capsule with fixed doses of the drug (25 mg and 50 mg). Syrup was prepared in sucrose solution at 5 mg/mL concentration. Bioactivity and phytochemical assays were used for dosage form stability and uniformity evaluations before and after 3- and 6- months incubation. Pharmacological bioassay method was designed to determine the bioactivity of the products before and after incubation. Pharmacological effects of the prepared capsule and syrup were determined on rat isolated ileum and intestinal meal transit, respectively. Results: In this study, D. kotschyi extract was effectively formulated as capsule and syrup for oral consumption. Environmental and aging factors had no significant effect on the total flavonoid or phenolic contents or bioactivity of the manufactured products. Furthermore, the ingredients used in the formulation had no effect on the bioactivity of the active substances in the extract. Conclusion: The standard oral dosage forms prepared from D. kotschyi extract can be used for clinical trials. In addition, we introduced a reliable bioassay technique, which might be applied for the evaluation of herbal medicines with antispasmodic activities.

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“…MN method proceeded according to the previously described procedure ( 6 ). The mice fasted for 4 h before the experiment and were treated as follows (6 mice in each group): ( 1 ) Control group, mice received a single i.p injection of normal saline and cosolvent (tween 2%); ( 2 3 4 ) indomethacin treatment groups, mice received a single i.p dose of indomethacin at 10, 20, and 40 mg/kg ( 17 ); ( 5 6 7 ) indomethacin-curcumin treatment groups, mice received a single i.p dose of curcumin at 100 mg/kg ( 18 ) 30 min before a single i.p dose of indomethacin 10, 20, and 40 mg/kg.…”

Section: Methodsmentioning

confidence: 99%

“…Neutrophils can play a role in wound formation through the release of tissue-damaging proteases and reactive oxygen metabolites. This process is important in the pathogenesis of gastric ulcers process ( 7 ). Moreover, indomethacin can cause ROS production, and ROS formation plays an important role in the specific and idiosyncratic toxicity of indomethacin, such as bone marrow toxicity or liver inflammation ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo assessment of indomethacin-induced genotoxicity: protection by curcumin

Zamani,

Klour,

Shekarsarayi

et al. 2024

Research in Pharmaceutical Sciences

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Background and purpose: Indomethacin is one of the most widely used non-steroidal anti-inflammatory drugs. This study aimed to investigate the protective effects of curcumin against indomethacin-induced genotoxicity. Experimental approach: For in vitro studies, human peripheral blood lymphocytes were obtained from a healthy volunteer and treated for 24 h as follows: vehicle control, indomethacin at 100 and 200 μΜ, indomethacin (100 μM and 200 μM) plus curcumin (27 μM). For in vivo experiments, mice received a single i.p dose of curcumin (100 mg/kg) and after 30 min genotoxicity induction was carried out by a single i.p injection of indomethacin at 10, 20, and 40 mg/kg. After 24 h, bone marrow cells were obtained from mice femurs. Genotoxicity was evaluated using a micronucleus assay. Oxidative damage was also inspected both in vitro and in vivo. Findings/Results: In-vitro studies indicated that co-treatment with curcumin caused a significant decrease in the average micronuclei percentage and MDA level, and a significant increase in GSH concentration compared to the groups treated only with indomethacin. In-vivo findings revealed that pretreatment with curcumin induced a significant increase in the average ratio of polychromatic erythrocyte/normochromic erythrocyte, GSH concentration and caused a significant decrease in the average percentage of micronuclei and MDA level, in comparison with the group treated only with indomethacin. Conclusion and implications: Curcumin attenuated indomethacin-induced genotoxicity both in vitro and in vivo. These effects might be partially exerted by decreasing oxidative stress. Further studies are required to elucidate the exact genoprotective mechanism of curcumin against indomethacin.

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Evaluation of the effect of hydroalcoholic and flavonoid-enriched extracts of Dracocephalum kotschyi on indomethacin-induced gastric ulcer in rats

Cited by 7 publications

References 30 publications

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

The Potential Protective Eff ects of Pirfenidone on Diclofenac Sodium Induced Gastric Ulcer in a Rat Model

Bioassay standardization of drug dosage form prepared from hydroalcoholic extract of Dracocephalum kotschyi

In vitro and in vivo assessment of indomethacin-induced genotoxicity: protection by curcumin

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