Protective effect of thiourea, a hydroxyl-radical scavenger, on curcumin-induced chromosomal aberrations in an in vitro mammalian cell system

Araújo, Maria Christina Barbosa de; Antunes, Lusânia Maria Greggi; Takahashi, Catarina Satie

doi:10.1002/1520-6866(2001)21:2<175::aid-tcm6>3.0.co;2-v

Cited by 35 publications

(25 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In spite of curcumin is a natural antioxidant known to possess therapeutic properties and has been reported to scavenge free radicals and to inhibit clastogenesis in mammalian cells [7]. Curcumin has been reported to induce a significant increase in the frequency of chromosomal aberrations in Chinese hamster ovary (CHO) cells [8]. So it was necessary to evaluate the genotoxic effects and histochemical changes induced in male rat cells as a result to oral administration of different doses of curcumin for four weeks.…”

Section: Introductionsupporting

confidence: 74%

“…In kidney cells, also results showed that the curcumin induced significant decrease (p<0.01) in the mean value of DNA content than in control (table 4 and fig 6). As regard to PAS + ve material, curcumin caused significant decrease (p<0.01) in the level of PAS in both liver and kidney tissues of all groups as compared to control as shown in (tables 5, 6 and fig 7,8). while in renal tubule, all treated groups revealed moderate reaction in the basement membrane and brush border.…”

Section: Histological and Histochemical Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cytogenetical and histochemical studies on curcumin in male rats

Makawy¹,

Sharaf²

2006

WIT Transactions on Biomedicine and Health, Vol 10

View full text Add to dashboard Cite

Curcumin is a major component of the curcuma species, commonly used as a yellow coloring and flavoring agent in foods. Curcumin has been demonstrated to have potent antioxidant, anti-inflammatory activity and has shown anticancer properties in many rodent models. There is the perception that since compounds are natural, they are devoid of toxicity and safe to use. Some of the active compounds in supplements have inherent toxicity. In this study, five doses (0.5, 5, 10, 25 and 50 mg/kg/daily) of curcumin spice were orally administered to male rats daily for four weeks. The effect of curcumin was studied genetically by evaluation of chromosomal aberrations and micronucleus formation in bone marrow cells of male rats. Histopathological and histochemical investigations were studied in different tissues (liver, kidney) of males. The cytogenetical results showed that curcumin caused a statistically significant dose-dependent increase in the number of micronucleated polychromatic erythrocytes (MNPCEs) and in the frequencies of total chromosomal aberrations over the control. Also, results showed that there were significant differences between positive control and all curcumin-tested doses, except the dose of 50 mg/kg showed no significant difference. Histopathological results showed different degrees of alterations, as manifested by vacuolar degeneration in hepatocytes, tubular degeneration in renal tissues. We conclude that over use of curcumin spice may cause genotoxical and histopathological effects.

show abstract

Section: Introductionsupporting

confidence: 74%

Section: Histological and Histochemical Resultsmentioning

confidence: 99%

Cytogenetical and histochemical studies on curcumin in male rats

Makawy¹,

Sharaf²

2006

WIT Transactions on Biomedicine and Health, Vol 10

View full text Add to dashboard Cite

show abstract

“…6B). This characteristic quartet signal was almost completely suppressed by thiourea (data not shown), a specific • OH scavenger,19,20 suggesting that the quartet signal indicates a DMPO-OH spin adduct. On the other hand, H 2 O 2 -induced DMPO-OH signal was slightly reduced by GST, and markedly reduced by GST-DJ-1 (Fig.…”

Section: Resultsmentioning

confidence: 93%

Oxidative Stress Induction of DJ‐1 Protein in Reactive Astrocytes Scavenges Free Radicals and Reduces Cell Injury

Yanagida

Tsushima

Kitamura

et al. 2009

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Astrocytes, one of the predominant types of glial cells, function as both supportive and metabolic cells for the brain. Under cerebral ischemia/reperfusion-induced oxidative conditions, astrocytes accumulate and activate in the ischemic region. DJ-1 has recently been shown to be a sensor of oxidative stress in living cells. However, the function of astrocytic DJ-1 is still unknown. In the present study, to clarify the effect of astrocytic DJ-1 protein under massive oxidative insult, we used a focal ischemic rat model that had been subjected to middle cerebral artery occlusion (MCAO) and reperfusion. We then investigated changes in the distribution of DJ-1 in astrocytes, DJ-1 release from cultured astrocytes, and the effects of recombinant DJ-1 protein on hydrogen peroxide (H2O2)-induced death in normal and DJ-1-knockdown SH-SY5Y cells and on in vitro scavenging of hydroxyl radicals (•OH) by electron spin resonance spectrometry. At 24 h after 2-h MCAO and reperfusion, an infarct lesion was markedly observed using magnetic resonance imaging and 2,3,5-triphenyltetrazolium chloride staining. In addition, reactive astrocytes enhanced DJ-1 expression in the penumbral zone of the ischemic core and that DJ-1 protein was extracellularly released from astrocytes by H2O2 in in vitro primary cultures. Although DJ-1-knockdown SH-SY5Y cells were markedly vulnerable to oxidative stress, treatment with glutathione S-transferase-tagged recombinant human DJ-1 protein (GST-DJ-1) significantly inhibited H2O2-induced cell death. In addition, GST-DJ-1 protein directly scavenged •OH. These results suggest that oxidative stress induces the release of astrocytic DJ-1 protein, which may contribute to astrocyte-mediated neuroprotection.

show abstract

“…8 Selected 2-amino-thiazines and -thiazolines are important scaffolds in the development of aspartate beta-secretase enzyme (BACE-1) inhibitors, a therapeutic target for Alzheimer's disease, 9 and are common motifs in several bioactive compounds (Scheme 1b). Mechanistically, the ability of thioureas to act as reducing agent 10 and radical scavenger 11 suggests that this group may induce the release of CF 3 ˙ from the Togni reagent, 12 and serve as an S-source capable of adding on a C-centered radical. Here we report that thiourea-substituted alkenes undergo C–CF 3 followed by C–S bond formation with the Togni reagent and TFA.…”

Section: Introductionmentioning

confidence: 99%