Cytogenetical and histochemical studies on curcumin in male rats

Makawy, Aida I. El; Sharaf, Hafiza A

doi:10.2495/etox060171

Cited by 9 publications

(9 citation statements)

References 25 publications

(26 reference statements)

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“…The third group received an oral administration of curcumin at a dose of 50 mg/kg/bodyweight. This dose was selected on the basis of a previous study [ 19 ]. The fourth group received both curcumin (50 mg/kg/bodyweight) + propanil (20 mg/kg/body weight).…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Curcumin against the Liver Toxicity Caused by Propanil in Rats

Otuechere

Olateju

Animashaun

et al. 2014

International Scholarly Research Notices

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We investigated the protective effects of curcumin on propanil-induced alterations in biochemical indices in blood and liver of male Wistar rats. The study consisted of four treatment groups, with six animals each, designated as control, propanil (20mg/kg), curcumin(50 mg/kg), and curcumin (50 mg/kg) + propanil (20 mg/kg). Rats were administered their respective doses orally, every other day, for 28 days. Propanil administration elicited significant (P < 0.001) increases in plasma aspartate aminotransferase and alkaline phosphatase activities, by 24% and 56%, respectively, compared to the control. Treatment with propanil elevated bilirubin, creatinine, and total cholesterol levels in rats, but these were not significant relative to controls. Administration of propanil to rats significantly (P < 0.001) increased lipid peroxidation levels. However, catalase activity, vitamin C, and reduced glutathione levels were significantly reduced. Exposure to propanil did not produce any significant changes in packed cell volume, neutrophils, and leukocyte counts. The supplementation of curcumin attenuated the adverse effects of propanil intoxication by reducing lipid peroxidation levels and restored the levels of serum enzymes and reduced glutathione. The present study showed that propanil increased oxidative stress and altered some biochemical parameters in the rats but curcumin could afford some protection to attenuate propanil-induced toxicity in the liver.

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Section: Methodsmentioning

confidence: 99%

Protective Effect of Curcumin against the Liver Toxicity Caused by Propanil in Rats

Otuechere

Olateju

Animashaun

et al. 2014

International Scholarly Research Notices

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“…Curcumin spice caused a statistically significant dosedependent increase in the number of MNPCEs and in the frequencies of total chromosomal aberrations over the control in male rats which received a suspension of curcumin spice (not further specified) corresponding to 0.5, 5, 10, 25 and 50 mg/kg bw in 1 ml distilled water orally, daily, for four weeks (El-Makawy and Sharaf, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…injection with 25 mg/kg cyclophosphamide (positive control) or a suspension of curcumin spice (not further specified) corresponding to 0.5, 5, 10, 25 and 50 mg/kg bw in 1 ml distilled water orally, daily, for four weeks. Curcumin spice caused a statistically significant dose-dependent increase in the number of micronucleated polychromatic erythrocytes (MNPCEs) and in the frequencies of total chromosomal aberrations over the control (El-Makawy and Sharaf, 2006). The Panel noted that the curcumin tested was not adequately specified.…”

Section: Genotoxicitymentioning

confidence: 99%

Scientific Opinion on the re‐evaluation of curcumin (E 100) as a food additive

2010

EFS2

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The Panel on Food Additives and Nutrient Sources added to Food provides a scientific opinion re-evaluating the safety of curcumin (E 100). Curcumin has been previously evaluated by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) and the EU Scientific Committee on Food (SCF). In 2004 JECFA allocated an ADI of 0-3 mg/kg bw/day. The Panel was not provided with a newly submitted dossier and based its evaluation on previous evaluations, additional literature that became available since then and the data available following a public call for data. The Panel considered that the indications provided by the positive results for curcumin in several in vitro and in vivo tests for genotoxicity, especially those detecting chromosomal aberrations and DNA adducts should not be disregarded, and that the available in vivo genotoxicity studies were insufficient to eliminate the concerns regarding genotoxicity. The Panel noted that all statistically significant effects noted by NTP in a long-term carcinogenicity study in rats and mice refer to benign neoplastic lesions (adenomas) and that the incidences for malignant neoplastic lesions (carcinomas) did not reach statistical significance. The Panel also noted that the effects observed were not dose-dependent, were in line with historical control values and not consistent across sexes and/or species. The Panel agreed with JECFA that curcumin is not carcinogenic. The Panel also concluded that this eliminates the concerns over genotoxicity. The Panel concluded that the present database supports an ADI of 3 mg/kg bw/day based on the NOAEL of 250-320 mg/kg bw/day from the reproductive toxicity study for a decreased body weight gain in the F2 generation observed at the highest dose level, and an uncertainty factor of 100. The Panel concluded that at the maximum levels of use, intake estimates for 1-to 10-year old children at the mean and the high percentile (95 th ) are above the ADI in some European countries. The Panel noted that intake of curcumin from the normal diet amounts to less than 7% of the ADI of 3 mg/kg bw/day. © European Food Safety Authority, 2010 KEY WORDSCurcumin, E 100, CAS Registry Number Turmeric Yellow, Kurkum, INS no. 100(i), CI Natural Yellow 3, Diferoylmethane, EINECS number 207-280-5, Colour index number 75300. Curcumin (E 100) is a dicinnamoylmethane dye authorised as a food additive in the EU and was previously evaluated by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) in 1974, 1978, 1980, 1982, 1987, 1990, 1992, 1995, 2000, 2002 and 2004, and Curcumin consists of three principal colouring components. It consists essentially of curcumins i.e. the colouring principle (1E, 6E)-1, 7-bis-(4-hydroxy-3-methoxyphenyl)-hepta-1, 6-diene-3, 5-dione and its desmethoxy-and bis-desmethoxy-derivatives.Regarding the kinetics of curcumin, in animal studies it appeared that curcumin is rapidly metabolised and excreted (mainly via faeces). Studies in humans also reveal that it is unlikely that substantial concentrations of curcumin occur...

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“…In a male Wistar rat study (10 animals/dose) the animals were given a suspension of curcumin spice (not further described) orally, corresponding to 0.5, 5, 10, 25 and 50 mg/kg bw in 1 ml distilled water daily for 4 weeks (El-Makawy and Sharaf, 2006). Curcumin caused a statistically significant dose-dependent increase in the number of micronucleated polychromatic erythrocytes (MNPCEs) and in the frequencies of total CAs over the control (bone marrow cells were used in both studies).…”

Section: In Vivo Studiesmentioning

confidence: 99%