Protective effect of seminal plasma proteins on the degradation of ascorbic acid

Liu, Li; Dąbrowski, Konrad; Ciereszko, Andrzej

doi:10.1007/bf00929503

Cited by 23 publications

(7 citation statements)

References 25 publications

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“…The crossover point was between 1-2 mM ascorbate according to Rees and Slater (1997) . According to our previous work, inactivated antiproteinase activity of fish seminal plasma was not protected in vitro by exogenous ascorbic acid of 1-8 mM (Liu et al . 1995).…”

Section: Discussionmentioning

confidence: 94%

Dietary Ascorbyl Monophosphate Depresses Lipid Peroxidation in Rainbow Trout Spermatozoa

Liu

Ciereszko

Czesny

et al. 1997

Journal of Aquatic Animal Health

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This study was designed to analyze lipid peroxidation in spermatozoa of rainbow trout Oncorhynchus mykiss by an optimized thiobarbituric acid (TBA) method, and to evaluate the effect of graded levels of dietary antioxidant (ascorbic acid in the form of ascorbyl monophosphate, AP) on TBA values of spermatozoa . Sperm from rainbow trout fed diets supplemented with AP at 0, 110, or 870 mg/kg were sampled during the reproductive season . The group given the unsupplemented diet had the lowest ascorbic acid concentration in seminal plasma during most of the spermiation season compared with groups fed diets with AP While the ascorbic acid concentration in seminal plasma decreased toward the end of the reproductive season, spermatozoa malondialdehyde production, indicative of increasing peroxidation, tended to increase . At the end of the season, significant differences (P < 0 .05) were found in peroxidation levels in spermatozoa from fish fed different levels of ascorbic acid . The most abundant polyunsaturated fatty acid in sperm lipids, docosahexaenoic acid (C22 :6), was significantly lower in lipids from spermatozoa of the AP-devoid (control) group than in the 870-mg/kg supplement group . By the end of the reproductive season, spermatozoa from the control group had significantly higher peroxidation levels than did spermatozoa from fish given AP (110 and 870 mg/kg) . Thus, it is the first evidence in fish that dietary AP supplements can increase seminal plasma ascorbic acid concentrations, and it suggests that peroxidative damage to spermatozoa can be reduced during the reproductive season, which in turn may affect sperm fertilizing ability .Spermatozoa of sea urchins Strongylocentrotus intermedius, fish, and mammals, including hu-

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Section: Discussionmentioning

confidence: 94%

Dietary Ascorbyl Monophosphate Depresses Lipid Peroxidation in Rainbow Trout Spermatozoa

Liu

Ciereszko

Czesny

et al. 1997

Journal of Aquatic Animal Health

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“…Oxidative damage to some blood plasma anti-proteinase inhibitors led to a reduction, over 1000 times, of their inhibitory activity (Patterson 1991 ). We have found recently that seminal plasma anti-trypsin activity can be reduced by in vitro oxidation by hydrogen peroxide (Liu et al 1995). However, no protective effect of AA was found in vitro; ascorbic acid, at concentrations 1-8 mM, had pro-oxidant effect.…”

Section: Osmolality Of Seminal Plasmamentioning

confidence: 90%

Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen

Ciereszko

Liu

Dąbrowski

1996

Fish Physiol Biochem

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During the reproductive season, rainbow trout spermatozoa are stored in the sperm ducts for several months. There is no sperm production at this time since spermatogenesis is completed before spawning. To leam more about characteristics of semen during such a long storage, we analyzed changes in protein concentrations, anti-proteinase activity in seminal plasma and sperm aspartate aminotransferase activity during an extended reproductive period during which fish were fed diets supplemented with various ascorbic acid concentrations. Seminal plasma protein concentration and anti-proteinase activity declined toward the end of the reproductive season. These phenomena may be related to oncoming proteolytic events leading to degradation of the sperm. Protein concentrations and anti-proteinase activities were strongly correlated within groups of different ascorbic acid supplementations and several sampling dates (r=0.6-0.9 in most cases, p<0.05). Ascorbic acid deficiency resulted in a decrease in both parameter levels as compared to levels in groups with vitamin C supplement (p<0.08). Deficiency also resulted in lower stimulation of aspartate aminotransferase by an exogenous pyridoxal 5'-phosphate in comparison to fish fed vitamin C-supplemented diets (p<0.05). These results support earlier studies suggesting a protective role of ascorbic acid toward maintaining sperm quality.

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“…In seminal plasma and in spermatozoa of teleostei fish there are different types of antioxidants (Liu et al, 1995;Ciereszko et al, 2000;Lahnsteiner et al, 2010a), which are important for the in vivo maintenance of sperm viability, and that could have relevance for the practice of supplementation of the semen storage and cryopre-servation mediums to improve the quality of the spermatozoa. Ascorbic acid Metwally & Fouad, 2009) and Uric acid (Ciereszko et al, 1999) are considered important antioxidants in teleostei semen.…”

Section: Introductionmentioning

confidence: 99%

Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability

Ubilla¹,

Valdebenito²

2011

lajar

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ABSTRACT. The present investigation determined how different antioxidants incorporated into the sperm diluent for cold storage of semen affected sperm motility and spermatozoan fertility capabilities of the rainbow trout. For the evaluations, fresh semen (C) and semen that had been stored without diluents (T1) were used as control groups. The diluents were prepared using a base of UCT diluents (T2), adding grape polyphenol (0.1 g 100 mL -1 ) (T3), trolox C (0.1 g 100 mL -1 ) (T4), polyphenol (0.1 g 100 mL -1 ) plus trolox (0.1 g 100 mL -1 ) (T5), and vitamin C (0.018 g 100 mL -1 ) (T6). The incorporation of antioxidants into sperm diluents prolongs motility and fertility of rainbow trout semen. The results show that by day two, all of the treatments showed level 5 sperm motility. After seven days of storage, only T3 and T6 dropped to level 4 sperm motility. The duration of flagellate activity on this day was maximal for T3 with 36.87 ± 0.51 s and minimal for T6 with 29.78 ± 0.52 s. On day seven, fertility was maintained with no statistically significant differences between the control and T2 (92.80 ± 0.62%), T3 (83.66 ± 2.52%), T4 (90.46 ± 1.60%), T5 (83.57 ± 2.75%), and T6 (83.57 ± 2.30%). By days 10 and 17 of storage, the fertility of T1 was zero and that of T2 was significantly lower than the control group. On day 17, the highest percentage of fertilization was 97.38 ± 1.85% for T5 and the lowest value was 64.69 ± 3.76% for T2. The results allow concluding that the sperm viability of semen stored with different antioxidants is significantly prolonged. Keywords: Oncorhynchus mykiss, polyphenol, trolox C, vitamin C, fertilizing capability, sperm motility.Uso de antioxidantes en el diluyente espermático para trucha arcoiris Oncorhynchus mykiss (Walbaum, 1792): efecto en la motilidad y capacidad fecundante RESUMEN. En la presente investigación se determinó el efecto en la motilidad espermática y la fertilidad del espermatozoide de trucha arcoiris, de diferentes antioxidantes incorporados en el diluyente espermático para el almacenamiento en frío de semen. Para las evaluaciones se utilizó como control semen fresco (C) y semen almacenado sin diluir (T1), los diluyentes fueron preparados utilizando como base el diluyente UCT (T2) al que se le incorporó polifenol de uva (0,1 g 100 mL -1 ) (T3), trolox C (0,1 g 100 mL -1 ) (T4), polifenol más trolox (0,1 g 100 mL -1 + 0,1 g 100 mL -1 ), respectivamente (T5) y vitamina C (0,018 g 100 mL -1 ) (T6). La incorporación de antioxidantes en los diluyentes espermáticos, prolongan la motilidad y fertilidad del semen de trucha arcoiris. Los resultados muestran que al día dos todos los tratamientos presentaban un nivel 5 de motilidad. Después de 7 días de almacenamiento, sólo T3 y T6 bajaron a un nivel 4 de motilidad. La duración de la actividad flagelar en este día fue máxima en T3 con 36,87 ± 0,51 s y mínima en T6 con 29,78 ± 0,52 s. Al día 7 la fertilidad se mantuvo sin diferencias estadísticamente significativas con el control en T2 (92,80 ± 0,62%), T3 (83,66 ± 2,52%), T4 (90...

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Protective effect of seminal plasma proteins on the degradation of ascorbic acid

Cited by 23 publications

References 25 publications

Dietary Ascorbyl Monophosphate Depresses Lipid Peroxidation in Rainbow Trout Spermatozoa

Dietary Ascorbyl Monophosphate Depresses Lipid Peroxidation in Rainbow Trout Spermatozoa

Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen

Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability

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