Protective effect of Schizandrin B against damage of UVB irradiated skin cells depend on inhibition of inflammatory pathways

Abstract

Schizandrin B is extracted from Schisandra chinensis (Turcz.) Baill. This study evaluated the photoprotective effect of Schizandrin B on oxidative stress injury of the skin caused by UVBirradiation and the molecular mechanism of the photoprotective effect of Schizandrin B, and we firstly found that Schizandrin B could block Cox-2, IL-6 and IL-18 signal pathway to protect damage of skin cells given by UVB-irradiation. In the research, we found that Schizandrin B can attenuate the UVB-induced toxicity on keratinocytes and dermal fibroblasts in human body, and can outstandingly eliminated intracellular ROS produced by UVB-irradiation. These results demonstrate that Schizandrin B can regulate the function of decreasing intracellular SOD's activity and increasing the expression level of MDA in HaCaT cells result from the guidance of UVB, and it markedly reduced the production of inflammatory factors such as Cox-2, IL-6 or IL-18, decreased the expression level of MMP-1, and interdicted degradation process of collagens in UVB-radiated cells. Therefore, skin keratinocytes can be effectively protected from UVB-radiated damage by Schizandrin B, and UVB-irradiation caused inflammatory responses can be inhibited by attenuating process of ROS generating.

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

“…The authors showed that the COX-2, IL-6, and IL-18 gene expression were gradually reduced with increasing concentration of schisandrin B, after UVB irradiation. Gao et al evaluated the effects of this compound on expression levels of MMP-1 mRNA and on the synthesis of type 1 pro-collagen after UVB exposure in fibroblasts; the results revealed that schisandrin B reduced MMP-1 gene expression and restored the synthesis of pro-collagen in concentration dependent way [ 82 ].…”

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice

“…The expression of MMPs promotes degradation of the ECM, and MMPs also play a role in skin cancer induced by UV radiation [36]. Through cell surface receptors, IL-1β and TNF-α not only promote the transmission of NF-κB signals, but also activate the transcription of activator protein 1-regulated genes by activating the mitogen-activated protein kinase signaling pathway, inducing the expression of MMPs [37]. Upon UVB radiation of the skin, keratinocytes can indirectly cause skin photoaging by causing the secretion of IL-1β and IL-6.…”

Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-κB, MAPKs and PI3K/Akt pathways and activating Nrf2/HO-1 signaling

“…Similarly, this compound exhibited anti-inflammatory activity in LPS-stimulated RAW 264.7 cells; in particular, it increased glutathione S-transferase activity and decreased glutathione levels, thereby suppressing edema; this indicates that the anti-inflammatory action of schisandrin A is closely associated with its antioxidant effect ( 28 ). Furthermore, several previous studies have reported that lignan-like substances with a structure similar to that of schisandrin A possess anti-inflammatory activities and excellent Nrf2-induction or ROS-scavenging abilities ( 29 – 34 ); however, the underlying mechanisms associated with the anti-inflammatory and antioxidant activity of schisandrin A have remained to be fully elucidated. Therefore, the present study evaluated the protective effect of schisandrin A and the underlying mechanisms associated with inflammation and oxidative stress in RAW 264.7 macrophages exposed to LPS derived from Escherichia coli ( E. coli ).…”