Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

Abstract

The polysaccharide of Polygonatum sibiricum (PSP)is one of the main active ingredients of Polygonatum Polygonatum in Liliaceae. It has anti-tumor, anti-aging, immune regulation, and antioxidative effects. Recent studies have shown that the Klotho gene and fibroblast growth factor-23 (FGF-23) have a common receptor, which is closely related to aging and highly expressed in kidney and meninges. Our study aimed to investigate the anti-aging effect of PSP on D-galactose-induced rats and its mechanism. D-galactose (120 mg Kg -1 ) and PSP (100 mg Kg -1 ) was used to intervene in rats, respectively. Then The changes of indexes of the natural aging-like model rats before and after PSP intervention were observed. We found that PSP could significantly improve the learning and memory abilities of rats and reverse the pathological changes of kidney tissues in rats. At the same time, PSP up-regulated the expression of Klotho mRNA and Klotho protein in the renal cortex, down-regulated the expression of FOXO3a mRNA and p-FOXO3a protein in renal tissue, and inhibited the expression of FGF-23 protein in the femur. Our studies suggest that PSP may play a role by regulating the Klotho-FGF23 endocrine axis, alleviating oxidative stress, and balancing calcium and phosphorus metabolism.Aging should be an irreversible, comprehensive, and gradual loss of the function of each cell and organ tissue in the body. It is the body in the disease and function of the high-risk state of decline. The affecting factor of aging is not merely one, but rather a result of a combination of factors synergies. Modern medical studies have shown that the essence of aging refers to the irreversible process of gradual degeneration of structure and function after biological development and maturation under normal conditions, with the increase of age, the decline of self-function, the decline of internal environmental stability and stress ability, and the gradual degeneration of structure and function. With the development of modern genetic technology, the mechanism of aging has been studied in depth. It has become one of the research hotspots in recent years to search for active substances with an anti-aging effect in Nature by using the mechanism of aging.Klotho gene, which is an anti-aging gene, was first discovered by Kuro-o 1 in 1997, and then Xiao 2 , Kurosu 3 , Yamamoto 4 and others studied the gene and its expression. The results showed that the Klotho gene and fibroblast growth factor-23 (fibroblast growth factor-23) have common receptors, which are strictly related to aging and highly expressed in kidney and meninges.In January 2018, Chinese and American research teams published their research results in Nature. The crystal structure of the ternary complex of anti-aging protein alpha klotho-fibroblast growth factor receptor 1c (fibroblast growth factor receptor 1c) -fibroblast growth factor 23 (fibroblast growth factor 23) was analyzed, and the mechanism of its regulation of aging was elucidated 5 . The above studies and reports indicate that the Klot...

Antioxidant Effect of Polygonatum sibiricum Polysaccharides in D-Galactose-Induced Heart Aging Mice

“…To the best of our knowledge, PSP could significantly improve the learning and memory abilities and reverse the pathological changes of kidney tissues in D-gal-induced natural aging-like model mice, suggesting the antiaging effect of PSP on D-gal-induced kidney and meninges aging [12]. The present study further demonstrated that the D-gal-induced heart aging and injury can be attenuated by oral administration of PSP.…”

“…Polygonatum sibiricum polysaccharide (PSP), the major pharmacology active constituent isolated from Polygonatum sibiricum , is widely applied to the therapy of cardiovascular diseases or osteoporosis [ 10 , 11 ]. Recently, PSP was reported to have an antiaging effect in the kidney and meninges, and it can alleviate kidney injury and declines of learning ability induced by D-galactose (D-gal) [ 12 ]. However, the effect of PSP on heart aging has not been investigated to date.…”

Identification of the protective effect of Polygonatum sibiricum polysaccharide on d -galactose-induced brain ageing in mice by the systematic characterization of a circular RNA-associated ceRNA network

“…In our study, the chronic injection of D-gal prolonged the escape latency and swimming distance of mice in an established behavioural test, thus indicating that the D-gal model is a well-established method for investigating anti-ageing pharmacological therapy. PSP is known to exhibit anti-ageing properties (Zheng 2020). In the present study, we found that the administration of PSP could reverse the dysfunction in learning and memory abilities caused by D-gal interference.…”

“…Polygonatum sibiricum polysaccharide (PSP) is the main active component of Polygonatum sibiricum Redout e and exhibits a range of biological functions, particularly with regards to antiageing effects (Zhao and Li 2015;Liu et al 2018). A previous study demonstrated that PSP may play an important role in delaying the ageing process by regulating the Klotho/FGF23 endocrine axis, inhibiting oxidative stress, and by modulating the metabolism of calcium and phosphorus (Zheng 2020). Several recent reports have also reported that PSP acts directly on brain tissue during the anti-ageing process.…”

D-(+)-Galactose-induced aging: A novel experimental model of erectile dysfunction

“…In addition, this monosaccharide can react with amine groups in various proteins, forming advanced glycation products, and promoting oxidative stress [ 10 ]. Recent studies have demonstrated that chronic administration of D-(+)-galactose also leads to immune system dysregulation, sex hormone deficiencies, increasing inflammatory cytokine levels, cellular apoptosis, and diminished total antioxidant capacity [ 10 , 11 ]. Taken together, these effects mimic aging and impel the subject toward development of age-related diseases [ 12 ].…”