Identification of the protective effect of Polygonatum sibiricum polysaccharide on d -galactose-induced brain ageing in mice by the systematic characterization of a circular RNA-associated ceRNA network

Abstract

Context Polygonatum sibiricum polysaccharide (PSP), derived from Polygonatum sibiricum Delar. ex Redoute (Liliaceae), is known to be able to delay the ageing process. However, the specific mechanisms underlying these effects are not clear. Objective To investigate the mechanisms underlying the effects of PSP treatment on brain ageing by the application of transcriptomic analysis. Materials and methods Forty Kunming mice were randomly divided into four groups (control, d -galactose, low-dose PSP, high-dose PSP). Mice were administered d -galactose (50 mg/kg, hypodermic injection) and PSP (200 or 400 mg/kg, intragastric administration) daily for 60 days. Behavioural responses were evaluated with the Morris water maze and the profiles of circRNA, miRNA, and mRNA, in the brains of experimental mice were investigated during the ageing process with and without PSP treatment. Results PSP improved cognitive function during brain ageing, as evidenced by a reduced escape latency time ( p < 0.05) and an increase in the number of times mice crossed the platform ( p < 0.05). A total of 37, 13, and 679, circRNAs, miRNAs, and mRNAs, respectively, were significantly altered by PSP treatment (as evidenced by a fold change ≥2 and p < 0.05). These dysregulated RNAs were closely associated with synaptic activity. PSP regulated regulate nine mRNAs ( Slc6a5 , Bean1 , Ace , Samd4 , Olfr679 , Olfr372 , Dhrs9 , Tsc1 , Slc12a6 ), three miRNAs ( mmu-miR-5110 , mmu-miR-449a-5p , mmu-miR-1981-5p ), and two circRNAs ( 2:29227578|29248878 and 5:106632925|106666845 ) in the competing endogenous RNA (ceRNA) network. Discussion and Conclusions Our analyses showed that multiple circRNAs, miRNAs, and mRNAs responded to PSP treatment in mice experiencing brain ageing.

Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice

“…As one of the most important constituents of PS, PSP has various biological activities, including anti-tumor, antioxidant, anti-inflammatory, and immunomodulatory, and plays an important role in the treatment of various diseases [ 29 , 30 ]. There are many reports that PSP improves memory dysfunction in mice [ 31 , 32 ], although the effect of PSP on memory impairment after steaming has not been extensively studied and its mechanism is unclear. In our study, the physicochemical properties of PSPs were altered to varying degrees after steaming, and we used OFT and MWM to assess the ameliorative effects of the changes in the physicochemical properties of steamed PSPs on the impaired spontaneous motor function and learning memory function in D-gal-induced mice.…”

Hsa_circ_0076931 suppresses malignant biological properties, down-regulates miR-6760-3p through direct binding, and up-regulates CCBE1 in glioma

“…CircRNAs are thought to act as miRNA sponges that diminish the repressive effects of miRNAs on downstream molecules [30][31][32]. MiRNAs are single stranded RNAs composed of 20-25 nt that can change post-transcriptional gene expression [33,34].…”

A Review of Polygonatum Mill. Genus: Its Taxonomy, Chemical Constituents, and Pharmacological Effect Due to Processing Changes

“…Compared to PCP from the raw rhizome, the immunological activities of PCP after 2 and 4 h were greater on PCP. The longer the steaming duration (6–12 h), the more PCP was destroyed, which had a detrimental effect on the immune system [ 111 ]. In another study [ 112 ], IL-2, IL-6, TNF-α, and IFN-α secretions reversed to normal levels after treatment with the water-soluble PSP extracted from crude and wine-processed PSP in the immunosuppressive model for spleen-deficient mice.…”