Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide

Hou, Yongzhong; Zhao, Guohao; Yang, Jie; Yuan, Ying; Zhu, Guo Guang; Hiltunen, R.

doi:10.1016/j.lfs.2004.05.005

Cited by 97 publications

(62 citation statements)

References 28 publications

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“…In addition, there are several investigations reported suggesting that the EO from RCX could protect against DNA damage and apoptosis induced by ultraviolet B in mammalian cells (Jeong et al, 2009). Ethyl ether extract of RCX blocks endothelial cell damage induced by hydrogen peroxide (Hou et al, 2004). The polysaccharides from RCX also have antioxidant effects and cytotoxicity in human liver carcinoma cell line (Yuan et al, 2008).…”

Section: Research Articlementioning

confidence: 99%

Essential oil from rhizomes ofLigusticum chuanxionginduces apoptosis in hypertrophic scar fibroblasts

Zhang

et al. 2010

Pharmaceutical Biology

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Section: Research Articlementioning

confidence: 99%

Essential oil from rhizomes ofLigusticum chuanxionginduces apoptosis in hypertrophic scar fibroblasts

Zhang

et al. 2010

Pharmaceutical Biology

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“…L. wallichii is commonly used to treat cardiovascular diseases, 4,10,21) and some organic acids, including ferulic acid, sedanonic acid, folic acid, vanillic acid, and caffeic acid, have been purified from L. wallichii. 22,23) Additionally, Liang et al 24) reported that ligustilide and butylidenephthalide from L. wallichii significantly inhibited the vasoconstriction induced by NE and calcium chloride.…”

Section: Discussionmentioning

confidence: 99%

“…It has been widely used in Asian countries to increase coronary blood flow and systemic circulation, and to relieve stasis. 1,2) Previous studies have indicated that L. wallichii improves blood fluidity 3) and inhibits endothelial cell damage 4) and vascular smooth muscle cell proliferation. …”

mentioning

confidence: 99%

Endothelium-Independent Vasorelaxation by Ligusticum wallichii in Isolated Rat Aorta: Comparison of a Butanolic Fraction and Tetramethylpyrazine, the Main Active Component of Ligusticum wallichii

Kim

Rhyu

2010

Biological & Pharmaceutical Bulletin

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Ligusticum wallichii FRANCHAT (or Ligusticum chuanxiong hort; L. wallichii), a member of the Umbelliferaceae family, is a Chinese medicinal herb that is a common constituent in polypharmaceutical herbal drugs. It has been widely used in Asian countries to increase coronary blood flow and systemic circulation, and to relieve stasis. 1,2) Previous studies have indicated that L. wallichii improves blood fluidity 3) and inhibits endothelial cell damage 4) and vascular smooth muscle cell proliferation. 5)We demonstrated that an extract of L. wallichii showed endothelium-dependent acute relaxation in isolated rat thoracic aorta.6) The effect of L. wallichii was due to endothelial nitric oxide (NO). However, the tetramethylpyrazine (TMP)-induced vasorelaxant effect was not related to endothelial NO. TMP was isolated as a biologically active component from L. wallichii. Since 1970, TMP has been used in China for the treatment of patients with angina pectoris and cerebral ischemic syndromes. [7][8][9] Earlier pharmacological studies demonstrated that aortic vasorelaxation induced by TMP involved the opening of ATP-sensitive K ϩ channels and/or small-conductance Ca 2ϩ -activated K ϩ channels. [10][11][12] Endothelium-derived relaxing factor (EDRF) and the membrane potential are major components in modulating vascular tone.13) After EDRF, the most important molecule is probably NO,14,15) and K ϩ channels play an important role in the regulation of membrane potential.16) Five distinct types of K ϩ channel have been documented in arterial smooth muscle: voltage-dependent K ϩ (K V ) channels, large-conductance Ca 2ϩ -activated K ϩ (LK Ca ) channels, small-conductance Ca 2ϩ -activated K ϩ (SK Ca ) channels, inward rectifier K ϩ (K IR ) channels, and ATP-sensitive K ϩ (K ATP ) channels. 16) Additionally, changes in K ϩ channel activity to produce a subsequent alteration in the activity of calcium channels may result in vasorelaxation.17) Thus, the present study was performed to separate the active fraction, to examine the active fractioninduced changes in membrane potential, the possible underlying mechanisms, and to perform comparisons with TMP using isolated endothelium-denuded rat aortic rings. MATERIALS AND METHODS Extraction and FractionationDried, sliced L. wallichii was obtained from a local market and ground with a commercial food mixer. The resulting powder was extracted under reflux with absolute ethanol for 1 h. The extract was evaporated under reduced pressure at low temperature (37-40°C) and lyophilized (LwEx). Extracts for all of the experiments were prepared from four batches of L. wallichii to assess variation in individual extracts. LwEx was fractionated successively with water and n-butanol, and each phase was concentrated and lyophilized into water-soluble (LwDw) and butanol-soluble (LwBt) fractions. The solid was stored at Ϫ20°C until use. LwEx and LwBt were dissolved in 30% ethanol and LwDw was dissolved in water to give final concentrations of 0.3 to 3.0 mg/ml in the bath. Voucher specimen nos. Lw-001 (LwE...

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“…According to several reports a few preparations (extract, infusion, decoction or oil) made with Osha roots' are commonly ingested for treating respiratory illnesses, fever, gastrointestinal disorders, sore throats and rheumatism; externally, root products are used to treat aches, pain, scorpion sting and skin infections (Appelt, 1985;Argueta et al, 1994;Bye, 1986;Galaviz et al, 1994;Olivas, 1999;Terrell & Fennell, 2009;Turi & Murch, 2010). Several phthalides, terpenoids, phenylpropanoids and coumarins have been isolated from the organic extract of L. porteri roots (Delgado et al, 1988;Hou et al, 2004;León & Delgado, 2012;Reza-Garduño, 1987;Ríos & Delgado, 1999;Zschoke et al, 1998). The essential oil was reported to contain sabinyl acetate, Z-ligustilide (1), Z-3-butylidenephthalide (2) and sabinol as the main components (Rivero-Cruz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive activity ofLigusticum porteripreparations and compounds

Juárez-Reyes

Ángeles-López

Rivero‐Cruz

et al. 2013

Pharmaceutical Biology

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Context: The roots and rhizomes of Ligusticum porteri Coulter & Rose (Apiaceae) are widely used in Mexican folk medicine for several purposes, including painful complaints. Objective: The main goal of this work was to demonstrate the analgesic action in mice of some preparations and major compounds from L. porteri. Materials and methods: The extracts, aqueous (AE) and organic (OE), the essential oil (EO) and major compounds (10-316 mg/kg) from L. porteri were evaluated as potential antinociceptive agents using the acetic acid-induced writhing and hot plate tests in ICR mice. Results: All preparations tested exhibited significant antinociceptive effect in the two animal pain models selected. AE and EO were more effective in the writhing test while OE had a better effect in the hot-plate model. On the other hand, Z-ligustilide (1) provoked an increment in the latency period to the thermal stimuli in the hot-plate test at a dose of 31.6 mg/kg, and a decrease in the number of abdominal writhes at 10 mg/kg. Z-3-butylidenephthalide (2) induced a dose-dependent antinociceptive action in the hot-plate assay; this compound was also effective for controlling the pain provoked by chemical irritation at the doses of 10 and 31.6 mg/kg. Finally, diligustilide (3) inhibited the number of writhing responses at all doses tested but was inactive in the hot-plate model. Conclusion:The present investigation provides in vivo evidence supporting the use of L. porteri to treat painful conditions in folk medicine.

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Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide

Cited by 97 publications

References 28 publications

Essential oil from rhizomes ofLigusticum chuanxionginduces apoptosis in hypertrophic scar fibroblasts

Essential oil from rhizomes ofLigusticum chuanxionginduces apoptosis in hypertrophic scar fibroblasts

Endothelium-Independent Vasorelaxation by Ligusticum wallichii in Isolated Rat Aorta: Comparison of a Butanolic Fraction and Tetramethylpyrazine, the Main Active Component of Ligusticum wallichii

Antinociceptive activity ofLigusticum porteripreparations and compounds

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