Protective effect of Withania somnifera root powder in relation to lipid peroxidation, antioxidant status, glycoproteins and bone collagen on adjuvant‐induced arthritis in rats

Abstract

The present investigation was carried out to evaluate the protective effect of Withania somnifera Linn. Dunal (family-Solanaceae), commonly known as Ashwagandha, on adjuvant-induced arthritic rats. Results were compared with those for Indomethacin, a nonsteroidal anti-inflammatory drug. Arthritis was induced by intradermal injection of complete Freund's adjuvant (0.1 mL) into the right hind paw of Wistar albino rats. Withania somnifera root powder (1000 mg/kg/day) and Indomethacin (3 mg/kg/day) were orally administered for 8 days (from 11th to 18th day) after adjuvant injection. The anti-arthritic effect of W. somnifera root powder was assessed by measuring changes in lipid peroxidation, antioxidant status, and glycoprotein levels in plasma and spleen of arthritic animals. In addition, cartilage degradation was also assessed by estimating bone collagen, and urinary constituents in arthritic animals. Results of the present investigation showed significant increase in the level of lipid peroxides, glycoproteins, and urinary constituents with the depletion of antioxidant status and bone collagen in arthritic animals. These biochemical alterations observed were ameliorated significantly by oral administration of W. somnifera root powder (1000 mg/kg body weight) in arthritic animals. The results of this study clearly indicate that W. somnifera root powder is capable of rectifying the above biochemical changes in adjuvant arthritis.

Effects of 1.25-Dihydroxycholecalciferol and Hydroalcoholic Extract of Withania Coagulans Fruit on Bone Mineralization and Mechanical and Histological Properties of Male Broiler Chickens

“…Whitania coagulans is known to contain estrogen-like withanolides (Atta-ur-Rahman et al, 2003;Prasad et al, 2010). As previously mentioned, plant secondary metabolites had beneficial effects on bone strength as a result of the prevention of connective tissue breakdown (Rasool & Varalakshmi, 2007). There were no differences in tibia stiffness among treatments as measured at 21 and 42 days of age.…”

Evaluation of a root extract gel from <i>Urtica dioica</i> (Urticaceae) as analgesic and anti-inflammatory therapy in rheumatoid arthritis in mice

“…For patients with RA, delivering drugs topically may not only increase patient compliance but also provide immediate and prolonged release of the drug [13]. The conventionally used NSAIDs in RA have also been developed into topical or transdermal forms for various benefits, including reduced adverse effects, reduced drug dosage, improved patient compliance, and prolonged drug release [6,8,20].…”

“…Herbal drugs have long been used in traditional medicine for various conditions of pain and inflammation including arthritis. Many previous studies have examined the use of herbal drugs in pain and inflammation management in RA [3,[9][10][11][12][13][14]. In a recent study, a polyherbal gel was prepared using seeds of Withania somnifera, rhizomes of Curcuma longa, and gel from Aloe vera leaves.…”

“…A variety of herbal drugs obtained from plants can suppress cell signaling intermediates mediating pain and inflammation in RA, such as various Chinese herbs (e.g., Aconiti Radix, Fagopyrum cymosum), Curcuma longa, and Withania somnifera [3,[9][10][11][12][13][14].…”

Phytomedicine in Joint Disorders

“…WS root powder had protective effect on bone collagen in experimental induced arthritis model in rats [111].…”