Protective effect ofCnestis ferrugineaand its active constituent on scopolamine-induced memory impairment in mice: A behavioral and biochemical study

Abstract

Context: Cnestis ferruginea Vahl ex DC (Connaraceae) (CF) is used in traditional African medicine in the management of CNS disorders. The degeneration and dysfunction of cholinergic neurons is closely associated with the cognitive deficits of Alzheimer's disease (AD) and oxidative stress has been implicated in its pathogenesis. However, the influence of C. ferruginea on the cholinergic system and oxidative stress parameters has not been explored. Objective: The present study investigates the effect of methanol root extract of C. ferruginea and its active constituent amentoflavone (CF-2) on memory, oxidative stress and acetylcholinesterase (AChE) activity in scopolamine-induced amnesia. Materials and methods: Mice were orally treated with CF (25-200 mg/kg), CF-2 (6.25-25 mg/kg) for three days and memory impairment was induced by intraperitoneal injection of scopolamine (3 mg/kg). Memory function was evaluated by passive avoidance and Morris water maze tests. Biochemical parameters of oxidative stress and cholinergic function were estimated in brain after the completion of behavioral studies. Results: Scopolamine caused memory impairment along with increased AChE activity and oxidative stress in mice brain. Oral administration of CF and CF-2 significantly prevented scopolamine-induced memory impairment, inhibited AChE and enhanced antioxidant enzyme activity in the brain following scopolamine injection as compared to vehicle administration in scopolamine (i.p.)-treated mice that were comparable to the effect of tacrine. Discussion and conclusion: The study demonstrated that C. ferruginea and its constituent have significant protective effect against scopolamine-induced memory deficits in mice that can be attributed to their antioxidant and antiAChE activity.

Neuroprotective effects of 20(S)‐protopanaxatriol (PPT) on scopolamine‐induced cognitive deficits in mice

“…AD is a neurodegenerative disease with complex pathology, involving cholinergic deficit and intracellular oxidative stress. SCOP, a nonselective muscarinic Ach receptor antagonist, blocks cholinergic transmission in the central nervous system and produces cognitive dysfunction, including long‐term and short‐term memory impairment (Ishola et al, ). SCOP also influences the brain oxidative status, which is imbalanced in AD, making the SCOP‐induced amnesia model relevant in screening and investigating antiamnesic agents.…”

Protective effects ofStachys sieboldiiMIQ extract in SK-N-SH cells and its memory ameliorative effect in mice

“…Based on the above results, 500 mg/kg SS administration was selected as a moderate dose for the scopolamine induced memory impairment experiment in this study. The passive avoidance test is generally used to evaluate memory in three steps (learning acquisition, memory retention, and the retrieval process) (Ishola et al, ). The SS improved learning and memory abilities as well as attenuated scopolamine induced amnesia mice.…”

Scopolamine induced behavioral and biochemical modifications and protective effect of Celastrus paniculatous and Angelica glauca in rats

“…[59,60] Scopolamine has been found to increase oxidative stress and impaired the anti-oxidative defense system. [61,62] Many clinical studies have reported strong evidence that oxidative stress in involved in the pathogenesis of Alzheimer's disease. The oxygen free radicals are implicated in the process of age related decline in the cognitive performance may be responsible for the development of Alzheimer's disease.…”