Abstract:This work aims to evaluate the potential effect of Stachys sieboldii MIQ (SS) against oxidative stress induced by hydrogen peroxide (H2O2) in SK‐N‐SH cells and memory enhancement in ICR mice using the passive avoidance test. The effect of SS in cell proliferation was measured by BrdU incorporation assay with dosages of SS from 0 to 2 mg/mL. SS partially antagonized the effect of 150 μM H2O2 on cell proliferation. In vivo study, SS (100, 250, and 500 mg/kg) was administered orally to mice for 4 weeks. SS led to… Show more
“…In agreement, we have previously reported protective effects of Stachys sieboldii MIQ (SS) against H 2 O 2 induced cytotoxicity in SK-N-SH cells (Human neuroblastoma cell line) and memory amelioration in mice [ 22 ]. Notably, an underlying molecular mechanism by which SS exert the neuroprotective effect has not yet been studied.…”
Section: Introductionsupporting
confidence: 81%
“…Alteration of cholinergic neurons in the hippocampus and frontal cortex are implicated in the cognitive disorders [ 3 ]. Acetylcholine synthesized by choline acetyl transferase in neurons and hydrolyzed by acetylcholine transferase after its release, is essential to memory process [ 22 ]. Therapies designed to reverse the cholinergic deficit in large measure based on their importance in cognition.…”
Section: Discussionmentioning
confidence: 99%
“…They are also a good source of oligosaccharides, proteins, and water-soluble vitamins (vitamin B complex), which are all major contributors to human nutrition [ 21 ]. Several reports have detailed the health benefits of Stachys sieboldii , such as its anti-inflammatory activity, ability to lower anoxia, immunosuppressive function, antinephritic activity, antioxidant, antimicrobial activity, they also improve memory and discomfort [ 20 , 21 , 22 , 23 ].…”
Cholinergic dysfunction, impaired brain-derived neurotrophic factor and cAMP response element binding protein (BDNF-CREB) signaling are one of the major pathological hallmarks of cognitive impairment. Therefore, improving cholinergic neurotransmission, and regulating the BDNF-CREB pathway by downregulating apoptosis genes is one strategy for inhibiting the etiology of dementia. This study evaluates the potential effects of Stachys sieboldii MIQ (SS) extract against cognitive dysfunction and its underlying mechanisms. SS supplementation for 33 days improved scopolamine-induced memory impairment symptoms in Morris water maze test and Y-maze test. SS reduced the acetylcholineesterase activity and significantly increase acetylcholine and cholineacetyltransferase activity in the brain. In the subsequent mechanism study, SS regulated the mRNA expression level of neuronal plasticity molecules such as (nerve growth factor) NGF, BDNF, CREB, and its downstream molecules such as Bcl-2 and Egr-1 by downregulating the neuronal apoptosis targets in both hippocampus and frontal cortex. Additionally, inward currents caused by SS in hippocampal CA1 neurons was partially blocked by the GABA receptor antagonist picrotoxin (50 μM), suggesting that SS acts on synaptic/extrasynaptic GABAA receptors. These findings indicate that SS may function in a way that is similar to nootropic drugs by inhibiting cholinergic abnormalities, and neuronal apoptosis targets and ultimately increasing the expression of BDNF-CREB.
“…In agreement, we have previously reported protective effects of Stachys sieboldii MIQ (SS) against H 2 O 2 induced cytotoxicity in SK-N-SH cells (Human neuroblastoma cell line) and memory amelioration in mice [ 22 ]. Notably, an underlying molecular mechanism by which SS exert the neuroprotective effect has not yet been studied.…”
Section: Introductionsupporting
confidence: 81%
“…Alteration of cholinergic neurons in the hippocampus and frontal cortex are implicated in the cognitive disorders [ 3 ]. Acetylcholine synthesized by choline acetyl transferase in neurons and hydrolyzed by acetylcholine transferase after its release, is essential to memory process [ 22 ]. Therapies designed to reverse the cholinergic deficit in large measure based on their importance in cognition.…”
Section: Discussionmentioning
confidence: 99%
“…They are also a good source of oligosaccharides, proteins, and water-soluble vitamins (vitamin B complex), which are all major contributors to human nutrition [ 21 ]. Several reports have detailed the health benefits of Stachys sieboldii , such as its anti-inflammatory activity, ability to lower anoxia, immunosuppressive function, antinephritic activity, antioxidant, antimicrobial activity, they also improve memory and discomfort [ 20 , 21 , 22 , 23 ].…”
Cholinergic dysfunction, impaired brain-derived neurotrophic factor and cAMP response element binding protein (BDNF-CREB) signaling are one of the major pathological hallmarks of cognitive impairment. Therefore, improving cholinergic neurotransmission, and regulating the BDNF-CREB pathway by downregulating apoptosis genes is one strategy for inhibiting the etiology of dementia. This study evaluates the potential effects of Stachys sieboldii MIQ (SS) extract against cognitive dysfunction and its underlying mechanisms. SS supplementation for 33 days improved scopolamine-induced memory impairment symptoms in Morris water maze test and Y-maze test. SS reduced the acetylcholineesterase activity and significantly increase acetylcholine and cholineacetyltransferase activity in the brain. In the subsequent mechanism study, SS regulated the mRNA expression level of neuronal plasticity molecules such as (nerve growth factor) NGF, BDNF, CREB, and its downstream molecules such as Bcl-2 and Egr-1 by downregulating the neuronal apoptosis targets in both hippocampus and frontal cortex. Additionally, inward currents caused by SS in hippocampal CA1 neurons was partially blocked by the GABA receptor antagonist picrotoxin (50 μM), suggesting that SS acts on synaptic/extrasynaptic GABAA receptors. These findings indicate that SS may function in a way that is similar to nootropic drugs by inhibiting cholinergic abnormalities, and neuronal apoptosis targets and ultimately increasing the expression of BDNF-CREB.
“…TG and TC from liver tissue were analyzed using a previously described method (Vijaya Abinaya et al, 2017). Tissue was homogenized using chloroform: methanol solution (2:1) was vortexed and centrifuged.…”
Section: Analysis Of Hepatic Lipid Profilementioning
confidence: 99%
“…There has been a rapid global increase in the use of herbs to treat various health problems over the past few decades (Ekor, 2013;Pichiah & Cha, 2015; Vijaya Abinaya, Kim, Lee, & Cha, 2017). Perilla frutescens, an edible plant with medicinal properties, belongs to the mint family Lamiaceae.…”
Purple perilla, rich in polyphenols such as rosmarinic acid, showed lipid lowering in adipocyte cells and prevented body weight gain in mice. Therefore we conclude that purple perilla may be a potential candidate for the development of functional foods or nutraceuticals in managing obesity in humans.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.