Protective Effect of Glyceollins in a Mouse Model of Dextran Sulfate Sodium-Induced Colitis

Abstract

Glyceollins, which are derived from daidzein in soybean in response to various stimuli or stresses, have been reported to activate antioxidant/detoxifying enzymes in a nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-dependent manner, in addition to exerting anti-inflammatory effects in murine macrophages. As the Nrf2 signaling pathway is known to antagonize nuclear factor (NF)-κB signaling, glyceollins likely have the potential to prevent or treat inflammatory bowel disease. Thus, this study was conducted to examine whether glyceollins could inhibit dextran sulfate sodium (DSS)-induced colitis in a mouse model. Ulcerative colitis (UC) was induced in male BALB/c mice by administering drinking water with 4% DSS for 5 days. Glyceollins (4 or 10 mg/kg of body weight) were orally administered 48 h before and after DSS treatment. We found that glyceollins alleviated histological colon damage and inflammation induced by DSS treatment. More specifically, glyceollins reduced plasma levels of inflammatory cytokines, such as tumor necrosis factor-α and interleukin-6, which were otherwise markedly increased by DSS treatment. Markers of tissue damage, including malondialdehyde and 8-hydroxy-2-guanosine, were significantly increased by DSS treatment; however, this effect was mitigated through concomitant treatment with glyceollins. Furthermore, nuclear accumulation of NF-κB p65 and the expression of inducible nitric oxide synthase were upregulated by glyceollins, consistent with the observed modulation of inflammatory markers. In conclusion, glyceollins have therapeutic potential for UC and merit further clinical study.

Protective Effects of Dioscorea batatas Flesh and Peel Extracts against Ethanol-Induced Gastric Ulcer in Mice

“…Collected stomach tissues were fixed in a 10% ( v / v ) formalin solution and then paraffin-embedded as previously described [ 22 ]. Paraffin blocks were sectioned at 5 μm thickness using a microtome (RM-2125 RT; Leica, Nussloch, Germany).…”

“…The plasma 8-OHdG concentration was determined using an enzyme-linked immunosorbent assay (ELISA) kit (Enzo Life Sciences, Inc., Farmingdale, NY, USA) as previously described [ 22 ]. Briefly, 50 μL of plasma samples, or a series of standard dilutions, were dispensed into each well of an immunoassay plate.…”

Novel (1E,3E,5E)-1,6-bis(Substituted phenyl)hexa-1,3,5-triene Analogs Inhibit Melanogenesis in B16F10 Cells and Zebrafish

“…Cultured cells were collected, rinsed, and subjected to NE-PER ® Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific) as previously described [ 32 , 33 ]. After protein quantification by Bradford assay, proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride (PVDF) membrane.…”

Periplaneta americana Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Rats by Keap1/Nrf-2 Activation, Intestinal Barrier Function, and Gut Microbiota Regulation

“…At present, an increasing number of researches ( Yang et al, 2016 ; Ahmed et al, 2017 ) have begun to focus on the role of Nrf2/Keap1 pathway in the inflammation-associated pathogenesis, as well as the suppression of pro-inflammatory signaling pathways. Besides, previous studies have demonstrated the decreased expression of Nrf2 in dextran sulfate sodium (DSS)-induced UC model and the important role of Nrf2 signaling pathway in UC treatment by some chemo-preventive agents ( Dae Park et al, 2017 ; Seo et al, 2017 ; Liu et al, 2018 ; Tan and Zheng, 2018 ).…”