Protective Effects of Dioscorea batatas Flesh and Peel Extracts against Ethanol-Induced Gastric Ulcer in Mice

Abstract

Gastric ulcer is a major digestive disorder and provoked by multifactorial etiologies, including excessive alcohol consumption. In this study, we examined the gastroprotective effect of aqueous and ethanolic extracts of Dioscorea batatas Decne (DBD; commonly called Chinese yam) flesh or peel against acidified ethanol-induced acute gastric damage in mice. Our findings demonstrated that oral supplementation of aqueous or ethanolic extracts of DBD flesh or peel before ulcer induction was significantly effective in macroscopically and histologically alleviating ethanol-induced pathological lesions in gastric mucosa, decreasing the plasma levels of inflammatory mediators, such as nitric oxide and interleukin-6, attenuating the gastric expression of cyclooxygenase-2, and increasing the gastric content of prostaglandin E2. In particular, pretreatment with the flesh extract prepared in 60% ethanol prominently decreased the expression of biomarkers of oxidative stress, including the plasma levels of 8-hydroxy-2-guanosine and malondialdehyde, and restored heme oxygenase-1 expression and superoxide dismutase activity in the stomach. Overall, these findings suggest that the oral supplementation with DBD extract, especially flesh ethanol extract, prior to excessive alcohol consumption, may exert a protective effect against ethanol-induced gastric mucosal damage in vivo, presumably through the activation of the antioxidant system and suppression of the inflammatory response.

Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

“…It is well established that PGE 2 is beneficial for duodenal ulcer healing wherein it acts as an important agent in gastrointestinal mucosal defense [32]. Furthermore, PGE 2 is an efficacious vasodilator which promotes ulcer healing by enhancing the release of mucus and bicarbonate and inhibiting the gastric acid secretion [33].…”

Quantitative Analysis of Bioactive Phenanthrenes in Dioscorea batatas Decne Peel, a Discarded Biomass from Postharvest Processing

“…In addition, its medicinal value is significant because it contains numerous bioactive constituents such as polysaccharides, allantoin, and polyphenols including flavonoids [3]. Previous studies have demonstrated that the Chinese yam exerts strong anti-oxidative [4], cholesterol-lowering [5], growth hormone-releasing activity [6], and protective effects against ethanol-induced gastric ulcers [7]. Recent studies have revealed intimate relations between bioactivities and their responsible secondary metabolites including allantoin, saponins and phenanthrenes.…”

In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit

“…Lung tissue homogenate was subjected to measurement of IL-10 (ELISA MAX standard set, Biolegend, San Diego, CA, USA) and prostaglandin E 2 (PGE 2 ) (Cayman Chemical, Ann Arbor, MI, USA) as per the manufacturer’s instructions. Liver tissue homogenate was used to determine the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) (Cat # ADI-EKS-350; Enzo Life Sciences, Farmingdale, NY, USA) and malondialdehyde (MDA) (Cat # ALX-850-287; Enzo Life Sciences, Inc., Farmingdale, NY, USA) as previously described [ 34 ]. The obtained values were normalized to the total amount of proteins.…”

“…Cytoplasmic and nuclear fractions of cultured cells and mouse tissues were prepared using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Rockford, IL, USA). The proteins in each fraction were blotted and their relative expression levels were determined as previously described [ 34 , 35 ]. Briefly, the primary antibodies used in this study were immunoglobulins against COX-2 (Cell Signaling Technology, Danvers, MA, USA), NF - κB (Bioworld Technology, St. Luis.…”