Novel (1E,3E,5E)-1,6-bis(Substituted phenyl)hexa-1,3,5-triene Analogs Inhibit Melanogenesis in B16F10 Cells and Zebrafish

Oh, Jisun; Kim, Jung-Eun; Jang, Jin Ho; Lee, Sang‐Woo; Park, Chul Min; Kim, Woo‐Keun; Kim, Jong-Sang

doi:10.3390/ijms19041067

Cited by 11 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After allowing to recover for additional 24 hr, the cells were harvested by Trypsin‐EDTA solution (Welgene, Gyeongsan, South Korea). Fractionation of nuclear and cytoplasmic proteins from the collected cells was performed by employing the NE‐PER ® Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Rockford, IL, USA) as previously described (Oh et al., ). The protein quantities in both cytoplasmic and nuclear fractions were determined by Bradford assay (Bio‐Rad Protein Assay, Bio‐Rad, Hercules, CA, USA) through the manufacturer's instruction.…”

Section: Methodsmentioning

confidence: 99%

Grape Peel Extract and Resveratrol Inhibit Wrinkle Formation in Mice Model Through Activation of Nrf2/HO‐1 Signaling Pathway

Kim

Averilla

et al. 2019

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

Considering the anti‐photoaging effect of antioxidant compounds, we investigated the protective capacity of grape peel extract (GPE) and resveratrol on ultraviolet (UV)‐induced skin wrinkle formation. Total phenolic, total anthocyanin, and total flavonoid content in GPE prepared from peel of Campbell Early variety were 23.96 ± 0.09, 3.27 ± 0.40, and 1.24 ± 0.09 mg/g dry weight, respectively. Additionally, trans‐resveratrol and piceid content of the resulting GPE were 117.14 ± 19.97 and 85.23 ± 8.89 µg/g dry weight, respectively. Oral administration of either 2 g GPE or 2 mg resveratrol per kg body weight in mice attenuated UVB‐induced epidermal thickening (the thickness was reduced by about 63% and 55% with GPE and resveratrol consumption prior to exposure to UVB, respectively, compared to only UVB‐treated condition) and had marginally protective effect on wrinkle formation of skin exposed to UVB. As introduction of either GPE or resveratrol induced Nrf2‐dependent antioxidant enzymes including heme oxygenase‐1 (HO‐1) in liver and skin as well as inhibited metalloproteinases, it is highly probable that the extract or resveratrol mitigated UVB‐induced photoaging through activation of Nrf2/HO‐1 signaling pathway. Practical Application This study proved that resveratrol and the extract of grape peel, a common by‐product of grape juice processing, provide effective protection from UV‐induced skin wrinkle formation. Therefore, grape peel extract, which contains an appreciable amount of bioactive compound resveratrol, can be utilized as functional food ingredient for the manufacture of inner beauty products.

show abstract

Section: Methodsmentioning

confidence: 99%

Grape Peel Extract and Resveratrol Inhibit Wrinkle Formation in Mice Model Through Activation of Nrf2/HO‐1 Signaling Pathway

Kim

Averilla

et al. 2019

Journal of Food Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…The obtained three layers were then tested for antimelanogenesis activity in murine melanoma B16-F10 cells. The mouse B16 melanoma cell is a sensitive, reliable, and feasible platform for screening large number of small molecular melanogenesis regulators [23,24,25]. At the concentrations of 25–100 μg/mL, the ethyl acetate-soluble layer demonstrated the most potent inhibitory activity, while slight inhibition was observed for either the n -butanol or water-soluble layers (Figure 1A) as evidenced by melanin contents in lyzed B16-F10 melanoma cells (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

Melanogenesis Inhibitors from the Rhizoma of Ligusticum Sinense in B16-F10 Melanoma Cells In Vitro and Zebrafish In Vivo

Cheng

Lee

Chu

et al. 2018

IJMS

View full text Add to dashboard Cite

The rhizoma of Ligusticum sinense, a Chinese medicinal plant, has long been used as a cosmetic for the whitening and hydrating of the skin in ancient China. In order to investigate the antimelanogenic components of the rhizoma of L. sinense, we performed an antimelanogenesis assay-guided purification using semi-preparative HPLC accompanied with spectroscopic analysis to determine the active components. Based on the bioassay-guided method, 24 compounds were isolated and identified from the ethyl acetate layer of methanolic extracts of L. sinense, and among these, 5-[3-(4-hydroxy-3-methoxyphenyl)allyl]ferulic acid (1) and cis-4-pentylcyclohex-3-ene-1,2-diol (2) were new compounds. All the pure isolates were subjected to antimelanogenesis assay using murine melanoma B16-F10 cells. Compound 1 and (3S,3aR)-neocnidilide (8) exhibited antimelanogenesis activities with IC50 values of 78.9 and 31.1 μM, respectively, without obvious cytotoxicity. Further investigation showed that compound 8 demonstrated significant anti-pigmentation activity on zebrafish embryos (10‒20 μM) compared to arbutin (20 μM), and without any cytotoxicity against normal human epidermal keratinocytes. These findings suggest that (3S,3aR)-neocnidilide (8) is a potent antimelanogenic and non-cytotoxic natural compound and may be developed potentially as a skin-whitening agent for cosmetic uses.

show abstract

“…We found that carvone decreased melanin content in a concentration-dependent manner ( Figure 1B,C). We recently conducted a phenotype-based approach to screening for compounds in B16F10 melanoma cells to find small-molecule inhibitors of melanin synthesis [16,17]. By this method, we found that carvone treatment markedly decreases melanin content.…”

Section: Carvone Decreases Melanin Content Of B16f10 Melanoma Cellsmentioning

confidence: 99%

Carvone Decreases Melanin Content by Inhibiting Melanoma Cell Proliferation via the Cyclic Adenosine Monophosphate (cAMP) Pathway

2020

View full text Add to dashboard Cite

Melanin, which determines the color of the skin and hair, is initially synthesized to protect the skin from ultraviolet light; however, excessive melanin pigmentation caused by abnormal cell proliferation can result in various melanocytic lesions. Cyclic adenosine monophosphate (cAMP) is known to regulate cell cycle progression and consequently to inhibit the division of abnormally proliferating cells. In this work, we aimed to test whether carvone, a scent compound from plants, inhibits proliferation and subsequently reduces melanin content of melanoma cells and to determine whether its beneficial effects are mediated by the cAMP pathway. We found that carvone decreases melanin content and inhibits melanoma cell proliferation in a concentration-dependent manner. Meanwhile, it inhibited the activation of cell cycle-associated proteins such as cyclin-dependent kinase 1 (CDK1). Of note, the beneficial effects of carvone were abrogated by cAMP inhibition. Our findings indicate potential benefits of carvone for the treatment of melanomas and presumably other hyperpigmentation-related dermatological disorders such as melasmas, lentigines, and excessive freckles.

show abstract

Novel (1E,3E,5E)-1,6-bis(Substituted phenyl)hexa-1,3,5-triene Analogs Inhibit Melanogenesis in B16F10 Cells and Zebrafish

Cited by 11 publications

References 35 publications

Grape Peel Extract and Resveratrol Inhibit Wrinkle Formation in Mice Model Through Activation of Nrf2/HO‐1 Signaling Pathway

Grape Peel Extract and Resveratrol Inhibit Wrinkle Formation in Mice Model Through Activation of Nrf2/HO‐1 Signaling Pathway

Melanogenesis Inhibitors from the Rhizoma of Ligusticum Sinense in B16-F10 Melanoma Cells In Vitro and Zebrafish In Vivo

Carvone Decreases Melanin Content by Inhibiting Melanoma Cell Proliferation via the Cyclic Adenosine Monophosphate (cAMP) Pathway

Contact Info

Product

Resources

About