Protective effect of extract of Bletilla striata on isoflurane induced neuronal injury by altering PI3K/Akt pathway

Abstract

BackgroundPresent investigation evaluates the neuroprotective effect of Bletilla striata on isoflurane induced neuronal injury rat model.MethodologyNeuronal injury was induced by exposing the pups (P7) isoflurane (0.75%) in oxygen (30%) for the period of 6 hr and rats were treated with Bletilla striata at a dose of 35, 70 and 140 mg/kg, p.o. for the period of 21 days. At the end of protocol neurological score was estimated and serum concentration of inflammatory cytokines was estimated. Isolated brains tissue was prepared to perform immunohistochemical analysis, TUNEL assay and western blot assay.ResultsResult of the study reveals that treatment with BS significantly (p<0.01) reduces the neurological score compared to negative control group. Level of inflammatory cytokines in the serum and the expression of p-Akt, Bcl-xL and Bad protein were significantly attenuated in BS treated group. Moreover the cleaved caspase-3 and TUNEL positive cell was significantly (p<0.01) reduced in BS treated group compared to negative control group of rats.ConclusionPresent study concludes that ethanolic extract of Bletilla striata protects the neuronal injury by reducing apoptosis in isoflurane induced neuronal injury rats.

Eriodictyol corrects functional recovery and myelin loss in SCI rats

“…Recently, these herbs of traditional Chinese medicine have attracted researchers for treating SCI. Number of compound preparations [ 15 , 16 , 17 , 18 ] and herbs have shown a promising effect in treating SCI [ 19 , 20 , 21 , 22 ]. EDC is one of the active molecules used in traditional Chinese medicine.…”

Neuroprotective effects of miR-142-5p downregulation against isoflurane-induced neurological impairment

“…Sprague-Dawley (SD) rat pups (15-20 g) of postnatal day 7 (P7) were purchased from Changzhou Cavens Laboratory Animal Co. Ltd. All the rats were raised following the guidelines. The rats were randomly divided into four groups with 12 rats in each group: 1) control group: rats received regular air inhalation for 6 h; 2) isoflurane group: rats were exposed to isoflurane (1.5%) for 6 h [16]; 3) antagomir NC group: rats were given 2 nmol miR-142-5p antagomir NC by lateral cerebroventricular injection, and 30 min later, exposed to 1.5% isoflurane for 6 h; 4) miR-142-5p antagomir group: rats were given 2 nmol miR-142-5p antagomir by lateral cerebroventricular injection, and 30 min later exposed to 1.5% isoflurane for 6 h. After isoflurane treatment, six rats in each group were randomly selected and euthanized by decapitation, and the hippocampi tissues were collected for further experiments. And the remaining rats were used for following neurological scoring and Morris Water maze evaluation.…”

“…6 neonatal rats of each group were fed until day 14, and the behavior and motor changes of the experimental rats were evaluated using the 20-point neuron score, as previously reported [16]. The behavior evaluation included the response to the response to and circling of nociceptive stimuli, postural and walking reflexes, extremity tonus, performance in a smooth climbing platform, and consciousness.…”