Neuroprotective effects of miR-142-5p downregulation against isoflurane-induced neurological impairment

Abstract

Background: Isoflurane can lead to neuron damage to the developing brain, resulting in learning and memory disability. The aim of this study was to investigate the role of miR-142-5p on isoflurane-induced neurological impairment. Methods: The Morris water maze (MWM) test was performed to evaluate spatial learning and memory of rats. The expression level of miR-142-5p was measured using qRT-PCR. MTT assay was used to calculate the viability of hippocampal neuronal cells. The cell apoptosis was analyzed using Flow cytometric assay. Results: Isoflurane treatment led to the increase of neurological function score and escape latency, and the reduction of time spent in the original quadrant in rats. The expression level of miR-142-5p was increased significantly in isoflurane-treated rats. MiR-142-5p downregulation protected against isoflurane-induced neurological impairment, which was reflected by the decrease of neurological function score and escape latency, and the increase of time spent in the original quadrant. In vitro, downregulation of miR-142-5p alleviated isoflurane-induced neuron cell viability inhibition, and relieved isoflurane-induced cell apoptosis. Conclusions: MiR-142-5p downregulation plays a neuroprotective role in protecting against isoflurane-induced neurological impairment through regulating neuron cell viability and apoptosis. It provides a theoretical basis for the investigation of the mechanism underlying the effect on isoflurane-induced neurological impairment.

Recent progress on the role of non-coding RNA in postoperative cognitive dysfunction

“…Meanwhile, the relationship between several microRNA types [miR-124 (Yang et al, 2019), miR-153 (Shao et al, 2019), miR-106a (Zhang et al, 2020a), and miR-142-5p (Xie et al, 2020)] and isoflurane-induced neuronal apoptosis has been demonstrated. Chen et al (2016) found that 31 lncRNA molecules were significantly dysregulated (19 upregulated and 12 downregulated) in the hippocampus of sevofluranetreated neonatal mice.…”

“…Recently, an increasing number of microRNA molecules [miR-21 ( Twaroski et al, 2014 ), miR-206 ( Li et al, 2017 ), miR-34a ( Li et al, 2018 ), miR-215 ( Tang et al, 2020 ), miR-363-3p ( Yao and Zhang, 2020 ), miR-455-3p ( Zhu et al, 2020 ), miR-9-5p ( Zhang et al, 2022 ), and miR-582-5p ( Zhang et al, 2020b )] have been revealed to be associated with propofol-induced neuronal apoptosis. Meanwhile, the relationship between several microRNA types [miR-124 ( Yang et al, 2019 ), miR-153 ( Shao et al, 2019 ), miR-106a ( Zhang et al, 2020a ), and miR-142-5p ( Xie et al, 2020 )] and isoflurane-induced neuronal apoptosis has been demonstrated.…”

MicroRNA-424-5p Alleviates Isoflurane Anesthesia-Induced Neurotoxicity in Human Embryonic Stem Cell-Derived Neurons by Targeting FASN

“…Recently, miRNAs have presented potential influence on the regulation of neuronal injury induced by ISO. miR-142-5p depletion alleviates ISO-inhibited neuron viability and mitigated ISO-stimulated neuron apoptosis [ 20 ]. Upregulation of miR-133b attenuates ISO exposure-induced apoptosis of hippocampal neurons [ 21 ].…”

Repeated isoflurane exposures of neonatal rats contribute to cognitive dysfunction in juvenile animals: the role of miR-497 in isoflurane-induced neurotoxicity

“…Besides, some recent studies also indicated that miRNAs may participate in neurotoxicity induced by isoflurane. For instance, miR-142-5p was found to be upregulated in neurological impairment caused by isoflurane in neonatal rats, whereas the inhibition of miR-142-5p exerted neuroprotective effects against isoflurane, reflected by a better behavior performance [31]. Moreover, isoflurane facilitated the expression of miR-140-5p in diabetic rat hippocampus, which leads to the post-transcriptional suppression of sorting protein nexin 12, and the depletion of sorting nexin 12 subsequently contributed to isoflurane neurotoxicity [32].…”