Protective effect of dexpanthenol against cisplatin‑induced hepatotoxicity

Abstract

The aim of the present study was to investigate the protective effect of dexpanthenol (Dexp) against cisplatin (Cis)-induced hepatotoxicity. Thirty-two Sprague Dawley rats were divided into four groups: Control group (n=8), Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp), Cis group (n=8, 7 mg/kg/ip/single dose Cis) and Cis+Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp +7 mg/kg/ip/single dose Cis). MDA, CAT, GSH, GSH-Px, TOS, TAS, OSI, Total Nitrit, IL-1β, IL-6 and TNF-α levels were analyzed in liver tissue samples. After paraffinization of liver tissue samples, histopathological (congestion, loss of glycogen, number of Kupffer cells) and immunohistochemical (caspase-3 expression) parameters were assessed on the paraffinized liver sections. GSH, TAS, TOS, OSI, Tot Nit, L-Arginine, ADMA and SDMA levels were measured in the serum samples. Statistically significant differences were found between the groups in terms of all liver tissue biochemical parameters, with the exception of IL-1β and TNF-α levels. GSH, CAT, GSH-Px, TAS and Tot Nit levels were significantly higher in the Cis+Dexp group compared to the Cis group, whereas MDA, TOS, OSI and IL-6 levels were higher in the Cis group. Similarly, serum GSH, TAS, Tot Nit levels were higher in the Cis+Dexp group whereas TOS, L-Arginine, ADMA and SDMA levels were higher in Cis group. There were statistically significant differences between Control and Cis groups in terms of congestion increase, increase of glycogen loss, increase of Kupffer cell number and increase of caspase-3 expression (P<0.001). There was a statistically significant difference between the Cis and the Cis+Dexp groups in terms of histopathologic parameters, with the exception of congestion (P<0.001). To conclude, histopathological, immunohistochemical, and biochemical results of this study demonstrated that Dexp has a protective effect against Cis-induced hepatotoxicity.

Antioxidant Traits and Protective Impact of Vorinostat against Cisplatin Induced Hepatoxicity in Rats

“…Some samples showed peri-ductal inflammatory cell infiltrate. There finding was in full agreement with those previously reported by Bilgic et al [39]. They found that the most prominent alteration in the liver of CDDP-treated animals' group was sinusoidal congestion.…”

“…The quantitative assessment of the Caspase-3 immunoexpression in the liver revealed that it was significantly increased in this group compared to the control one. This finding was supported by this of Bilgic et al [39] who reported that CDDP group showed a significant increase in the caspase-3 expression in the liver [39].…”

“…They found that the most prominent alteration in the liver of CDDP-treated animals' group was sinusoidal congestion. They added that hepatocytes with eosinophilic cytoplasm and pyknotic nuclei were detected in some conditions [39].…”

Ginger juice prevents cisplatin‐induced oxidative stress, endocrine imbalance and NO/iNOS/NF‐κB signalling via modulating testicular redox‐inflammatory mechanism in rats

“…It unleashes its anticancer action through formation of cisplatin‐DNA adduct by intrastrand cross‐links due to interaction with cancer cell DNA purine bases eventually leading to p53 activation, cell cycle arrest and induction of apoptosis (Calcabrini, Maffei, Turrini, & Fimognari, 2020; Dasari & Bernard Tchounwou, 2014). Despite its potent antineoplastic efficacy, the constraint to its clinical utility is the development of organ toxicity, including nephrotoxicity, neurotoxicity, hepatotoxicity, myelosuppression, cardiotoxicity, pancreatic and testicular damage (Bami et al., 2017; Bilgic et al., 2018; El‐Hawwary & Omar, 2019; Stošić et al., 2020). In the emerging papers, CIS‐induced damage to testes and hormonal imbalance has been suggested a chief side effect because it has been clinically associated with infertility (Altuner, Gulaboglu, Yapca, & Cetin, 2013; Mercantepe, Unal, Tümkaya, & Yazici, 2018; Sabanegh & Ragheb, 2009).…”

Dexpanthenol may be a novel treatment for male androgenetic alopecia: Analysis of nine cases

“…Therefore, DXP has an “antioxidative,” “anti‐inflammatory” effect and a cellular repair capability 2,4 . To date, DXP has been used in numerous diseases including hepatotoxicity, wound healing, nephropathy without serious side effects 5‐7 …”