Protective Effect of Chemically Characterized Polyphenol-Rich Fraction from Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes on Carbon Tetrachloride-Induced Liver Injury in Mice

Abstract

Apteranthes europaea (Guss.) Murb. subsp. maroccana (Hook.f.) Plowes (A. europaea) is a medicinal plant widely used in traditional medicines to treat various diseases including hepatic pathogenesis. This study was conducted to evaluate the protective effect of chemically characterized polyphenol-rich fraction from A. europaea on carbon tetrachloride-induced liver injury in mice. The chemical characterization of A. europaea polyphenol-rich fraction was carried out using HPLC-DAD (high-performance liquid chromatography (HPLC) with a diode-array detector (DAD)). Carbon tetrachloride (CCl4) was used to induce liver injuries in mice as described in previous works. A polyphenol-rich fraction from A. europaea was used at a dose of 50 mg/Kg to study its hepatoprotective effect. Next, histopathological and biochemical alterations were investigated. The HPLC analysis revealed the presence of several phenolic compounds: gallic acid, methyl gallate, rutin, ferulic acid, and resorcinol. Regarding the mice treated with a polyphenol-rich fraction from A. europaea up to 50 mg/Kg and carbon tetrachloride, no significant biochemical nor histological alterations occurred in their liver; meanwhile, serious biochemical and histopathological changes were noted for liver recovered from the mice treated with carbon tetrachloride only. In conclusion, A. europaea extract is a promising source of hepatoprotective agents against toxic liver injury.

Traditional medicinal knowledge of plants used for cancer treatment by communities of mountainous areas of Fez-Meknes-Morocco

“…Previously published literature investigated the chemical compounds related to potential anticancer effects of some inventoried plant species in the current research such as hesperetin ( Choi, 2007 ), quercetin, myricetin ( Lu et al, 2006 ), ferulic acid ( Yang et al, 2015 ), and gallic acid ( Chen et al, 2009 ) contained in Apteranthes europaea (Guss.) Murb ( Amrati et al, 2020a ), ( Amrati et al, 2021 ), aristolochic acid I (AAsI) and aristolactam la (ALIa) in Aristolochia longa L. ( Hinou et al, 1990 ), thymoquinone, and alpha-hederin in Nigella sativa L.( Rooney and Ryan, 2005 ); ( Adamska et al, 2019 ), diallyl sulfide in Allium sativum L.( Wargovich, 1987 ), β-caryophyllene and α-humulene components in Marrubium vulgare L.( Fidyt et al, 2016 ); ( El Hadri et al, 2010 ) ; protodioscin in Trigonella foenum-graecum L. [31; 100] ( Ma et al, 2019 ) ; ( Alsemari et al, 2014 ) flavonoids and tannins in Artemisia herba-alba Asso ( Khlifi et al, 2013 ), quercetin in Allium cepa L. ( Votto et al, 2010 ), 6-gingerol, and 6-shogaol in Zingiber officinale Roscoe ( C.-H. Jeong et al, 2009 ) ; ( Wu et al, 2015 ) ; ( Cheng et al, 2011 ) ; Oleuropein in Olea europaea L. ( Ruzzolini et al, 2018 ), curcuminoids in Curcuma longa L. ( Anto et al, 1995 ) ; ( Hsiao et al, 2018 ) ; glabranine and naringenin in Linum usitatissimum L. ( Joseph et al, 2020 ), and Epicatechins in Camellia sinensis (L.) Kuntze ( Ravindranath et al, 2006 ); ( Bitu Pinto et al, 2015 ). The chemical structures of the mentioned compounds are presented in Fig.…”

Antioxidant, Anti-Inflammatory, and Analgesic Properties of Chemically Characterized Polyphenol-Rich Extract from Withania adpressa Coss. ex Batt

“…Other phytoconstituents in the genus Withania, particularly tannins, mucilages, alkaloids, coumarins, and free quinone, could be also responsible for the anti-inflammatory activity investigated in the present work. These compounds can act by preventing the synthesis of prostaglandins through cyclooxygenase [ 14 , 17 , 32 , 33 ].…”

“…The extraction of phenols was successfully conducted by using maceration as previously described [ 17 ]. To summarize, a total of 100 g of W. adpressa leaves was macerated with 300 mL of methanol.…”

“…The polyphenol-rich fraction was phytochemically characterized using the HPLC as described by Amrati [ 17 ], with minor modifications. The HPLC system was used for separation and identification of compounds.…”

Pharmacological Potential of Avicennia alba Leaf Extract: An Experimental Analysis Focusing on Antidiabetic, Anti-inflammatory, Analgesic, and Antidiarrheal Activity

“…Its crude extract has been shown to be effective against polymicrobial diseases [14]. A. alba leaves protect gastric mucosa from ethanol-induced damage [15], and the stem of the plant has been used to isolate and structurally elucidate new naphthoquinones and their analogues [1,6]. Plant and human pathogenic bacteria, particularly oral pathogens, were shown to be inhibited by A. alba [13,14].…”